Characterisation of the nuclear proteome of a dehydration-sensitive cultivar of chickpea and comparative proteomic analysis with a tolerant cultivar

Abstract

Water deficit or dehydration hampers plant growth and development, and shrinks harvest size of major crop species worldwide. Therefore, a better understanding of dehydration response is the key to decipher the regulatory mechanism of better adaptation. In recent years, nuclear proteomics has become an attractive area of research, particularly to study the role of nucleus in stress response. In this study, a proteome of dehydration-sensitive chickpea cultivar (ICCV-2) was generated from nuclei-enriched fractions. The LC-MS/MS analysis led to the identification of 75 differentially expressed proteins presumably associated with different metabolic and regulatory pathways. Nuclear localisation of three candidate proteins was validated by transient expression assay. The ICCV-2 proteome was then compared with that of JG-62, a tolerant cultivar. The differential proteomics and in silico analysis revealed cultivar-specific differential expression of many proteins involved in various cellular functions. The differential tolerance could be attributed to altered expression of many structural proteins and the proteins involved in stress adaptation, notably the ROS catabolising enzymes. Further, a comprehensive comparison on the abiotic stress-responsive nuclear proteome was performed using the datasets published thus far. These findings might expedite the functional determination of the dehydration-responsive proteins and their prioritisation as potential molecular targets for better adaptation.

Influence of abiotic stresses on plant proteome and metabolome changes

“…Another enzyme, glyoxalase, involved in the detoxification of R-oxoaldehydes, was also up-regulated in the tolerant variety. The lack of induction of major ROS catabolic enzymes may lead to hypersensitivity of plants during dehydration-induced oxidative stress (Subba et al 2013). The up-regulation of glyoxylate was also observed in rice root tissue in response to cold treatment (Lee et al 2009).…”

Comparative analysis of phytochemicals and nutrient availability in two contrasting cultivars of sweet potato (Ipomoea batatas L.)

“…However the outcome is affected by genotypic divergence, organ and tissues, developmental stages besides different environmental cues. The proteomics studies revealed the influence and efficacy of such approach to distinguish the landraces, populations, varieties and even species (Jaiswal et al, 2013;Subba et al, 2013).…”

Genome-Scale Transcriptomic Insights into Molecular Aspects of Abiotic Stress Responses in Chickpea

“…Based on an oligonucleotide microarray containing 6,164 unigenes, Wang et al (2012) reported a large number of unigenes differentially expressed in the root and leaf under drought stress. The proteomics approach has also identified abiotic stress-responsive proteins in chickpea (Pandey et al 2006;Subba et al 2013). Recently, based on phenotyping and genotyping of two intra-specific recombinant inbred line populations, at least nine quantitative trait locus (QTL) clusters for drought tolerance traits have been identified (Varshney et al 2014).…”