Characterisation of the nasal microbiota in granulomatosis with polyangiitis

Rhee, Rennie L.; Sreih, Antoine; Najem, Catherine; Grayson, Peter C.; Zhao, Chunyu; Bittinger, Kyle; Collman, Ronald G.; Merkel, Peter A.

doi:10.1136/annrheumdis-2018-213645

Cited by 38 publications

(37 citation statements)

References 45 publications

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“…Another pathology of autoimmune origin associated with effects on the respiratory system is Wegener's granulomatosis (WG), which is a systemic vasculitis with anti-neutrophil cytoplasmic antibodies (ANCAs), frequently affecting the upper respiratory system and having few effects on the lower airways. Evaluation of the nasal microbiome found that patients with WG have a low relative abundance of Propionibacterium acnes and Staphylococcus epidermidis; in patients with active disease, there is less abundance of the Malasseziales fungus compared to patients in remission [75].…”

Section: Pulmonary Microbiome In Interstitial Lung Diseasesmentioning

confidence: 99%

Lung Microbiome Participation in Local Immune Response Regulation in Respiratory Diseases

et al. 2020

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The lung microbiome composition has critical implications in the regulation of innate and adaptive immune responses. Next-generation sequencing techniques have revolutionized the understanding of pulmonary physiology and pathology. Currently, it is clear that the lung is not a sterile place; therefore, the investigation of the participation of the pulmonary microbiome in the presentation, severity, and prognosis of multiple pathologies, such as asthma, chronic obstructive pulmonary disease, and interstitial lung diseases, contributes to a better understanding of the pathophysiology. Dysregulation of microbiota components in the microbiome–host interaction is associated with multiple lung pathologies, severity, and prognosis, making microbiome study a useful tool for the identification of potential therapeutic strategies. This review integrates the findings regarding the activation and regulation of the innate and adaptive immune response pathways according to the microbiome, including microbial patterns that could be characteristic of certain diseases. Further studies are required to verify whether the microbial profile and its metabolites can be used as biomarkers of disease progression or poor prognosis and to identify new therapeutic targets that restore lung dysbiosis safely and effectively.

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Section: Pulmonary Microbiome In Interstitial Lung Diseasesmentioning

confidence: 99%

Lung Microbiome Participation in Local Immune Response Regulation in Respiratory Diseases

et al. 2020

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“…eDNA becomes an efficient tool for monitoring eukaryotes when mitochondrial genes such as 12S rRNA are targeted 10,13 . For bacterial detection, 16S rRNA gene partial fragments have been targeted for the analysis of environmental and human-associated microbiomes 14–17 . Environmental Leptospira DNA has been successfully barcoded by using 16S rRNA, flaB , lipL32 or secY genes 18–22 .…”

Section: Introductionmentioning

confidence: 99%

Environmental DNA metabarcoding to detect pathogenic Leptospira and associated organisms in leptospirosis-endemic areas of Japan

Mizuyama

Sato

Minamoto

et al. 2019

Sci Rep

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Leptospires, which cause the zoonotic disease leptospirosis, persist in soil and aqueous environments. Several factors, including rainfall, the presence of reservoir animals, and various abiotic and biotic components interact to influence leptospiral survival, persistence, and pathogenicity in the environment. However, how these factors modulate the risk of infection is poorly understood. Here we developed an approach using environmental DNA (eDNA) metabarcoding for detecting the microbiome, vertebrates, and pathogenic Leptospira in aquatic samples. Specifically, we combined 4 sets of primers to generate PCR products for high-throughput sequencing of multiple amplicons through next-generation sequencing. Using our method to analyze the eDNA of leptospirosis-endemic areas in northern Okinawa, Japan, we found that the microbiota in each river shifted over time. Operating taxonomic units corresponding to pathogenic L . alstonii , L . kmetyi , and L . interrogans were detected in association with 12 nonpathogenic bacterial species. In addition, the frequencies of 11 of these species correlated with the amount of rainfall. Furthermore, 10 vertebrate species, including Sus scrofa , Pteropus dasymallus , and Cynops ensicauda , showed high correlation with leptospiral eDNA detection. Our eDNA metabarcoding method is a powerful tool for understanding the environmental phase of Leptospira and predicting human infection risk.

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“…As for AAV, a study using quantitative culture experiments reported that Staphylococcus aureus in BALF was particularly associated with patients with GPA compared with patients with idiopathic pulmonary fibrosis (IPF) 3 . One study using nasal swabs reported decreased Propionibacterium acnes and Staphylococcus epidermidis in patients with GPA when compared with healthy controls 59 . Another study using nasal swabs reported increased relative abundance of Planococcaceae family and decreased Moraxellaceae, Tissierellaceae, Staphylococcaceae, and Propionibacteriaceae families in GPA when compared with healthy controls 48 .…”

Section: Discussionmentioning

confidence: 99%

“…We enumerate 4 probable causes of theses inconsistencies of results between ours and previous researches. First, because previous studies of these bacteria used the nasal swabs 48,59,60 , nasal carriage 2 , blood samples 6 , or lymph nodes 8,63 , these results may be attributable to the differences of specimens. Second, we compared AAV with sarcoidosis although one study compared GPA with IPF 3 and others compared AAV or sarcoidosis with healthy controls.…”

Section: Discussionmentioning

confidence: 99%

Comparison of lung microbiota between antineutrophil cytoplasmic antibody-associated vasculitis and sarcoidosis

Fukui

Morimoto

Ichinose

et al. 2020

Sci Rep

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Microbial involvement in the pathogenesis have been suggested in both antineutrophil cytoplasmic antibody-associated vasculitis (AAV) and sarcoidosis, both of which have lung involvement. However, exhaustive research to assess the bacteria in the lung in AAV and in sarcoidosis have not been performed. We sought to elucidate the distinct dysbiotic lung microbiota between AAV and sarcoidosis. We used 16S rRNA gene high-throughput sequencing to obtain the bacterial community composition of bronchoalveolar lavage fluid (BALF) in patients with AAV (n = 16) compared to patients with sarcoidosis (n = 21). The patients had not undergone therapy with immunosuppressive medication when their BALF was acquired. No difference was observed in α-diversity between patients with AAV and patients with sarcoidosis when using all the detected taxa. We defined the taxa of the oral cavity by using the data of oral microbiota of healthy individuals from the Human Microbiome Project (HMP). The analysis using only oral taxa made the difference in α-diversity between AAV and sarcoidosis clearer compared with those using all the detected taxa. Besides, the analysis using detected taxa except for oral taxa also made the difference in α-diversity between AAV and sarcoidosis clearer compared with those using all the detected taxa. A linear negative relationship between the α-diversity and Birmingham vasculitis activity score (BVAS) was detected in the AAV group. The observed p-value for the effect of the disease groups on the ß-diversity was small while the effect of other factors including sex and smoking status did not have small p-values. By excluding oral taxa from all the detected taxa, we found a cluster mainly consisted of sarcoidosis patients which was characterized with microbial community monopolized by Erythrobacteraceae family. Our results suggested the importance of considering the influence of oral microbiota in evaluating lung microbiota. Granulomatosis with polyangiitis (GPA) and microscopic polyangiitis (MPA) are forms of antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV), a systemic disease affecting multiple organs including the lungs and kidneys. The production of ANCA, playing a pathogenetic role in AAV, is thought to be initiated by exogenous or endogenous antigens including microbes, drugs, or dysregulated autoantigen expression 1. Patients

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Characterisation of the nasal microbiota in granulomatosis with polyangiitis

Cited by 38 publications

References 45 publications

Lung Microbiome Participation in Local Immune Response Regulation in Respiratory Diseases

Lung Microbiome Participation in Local Immune Response Regulation in Respiratory Diseases

Environmental DNA metabarcoding to detect pathogenic Leptospira and associated organisms in leptospirosis-endemic areas of Japan

Comparison of lung microbiota between antineutrophil cytoplasmic antibody-associated vasculitis and sarcoidosis

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