Characterisation of the mating-type locus in the genus Xanthoria (lichen-forming ascomycetes, Lecanoromycetes)

Scherrer, Sandra; Zippler, Undine; Honegger, Rosmarie

doi:10.1016/j.fgb.2005.09.002

Cited by 44 publications

(54 citation statements)

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“…One of or both APN1 and SLA2 have been reported to neighbor MAT loci in lineages including the hemiascomycete yeasts (2), related Aspergillus species (13), and distant euascomycetes (39). A gene arrangement identical to the N. fischeri MAT1 architecture is known to exist in Neurospora crassa, Yarrowia lipolytica, Fusarium and Xanthoria species, A. fumigatus, and A. oryzae (39,13).…”

Section: Analysis Of N Fischeri Mat1-and Mat2-flanking Regions Blasmentioning

confidence: 99%

“…A gene arrangement identical to the N. fischeri MAT1 architecture is known to exist in Neurospora crassa, Yarrowia lipolytica, Fusarium and Xanthoria species, A. fumigatus, and A. oryzae (39,13). This consistent gene arrangement suggests that APN1 and SLA2 were closely linked with the ancestral ascomycete MAT locus progenitor.…”

Section: Analysis Of N Fischeri Mat1-and Mat2-flanking Regions Blasmentioning

confidence: 99%

“…These are located within the genome at mating-type (MAT) loci and determine mating compatibility and incompatibility (3,7). In euascomycetes, there is most commonly a single MAT locus (termed MAT1) (39). In obligately outbreeding heterothallic species, which require a partner to mate, two nonallelic versions of the locus, termed idiomorphs, are found in isolates with complementary mating types (15,27).…”

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DNA Sequence Characterization and Molecular Evolution of MAT1 and MAT2 Mating-Type Loci of the Self-Compatible Ascomycete Mold Neosartorya fischeri

2007

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Degenerate PCR and chromosome-walking approaches were used to identify mating-type (MAT) genes and flanking regions from the homothallic (sexually self-fertile) euascomycete fungus Neosartorya fischeri, a close relative of the opportunistic human pathogen Aspergillus fumigatus. Both putative alpha-and high-mobilitygroup-domain MAT genes were found within the same genome, providing a functional explanation for selffertility. However, unlike those in many homothallic euascomycetes (Pezizomycotina), the genes were not found adjacent to each other and were termed MAT1 and MAT2 to recognize the presence of distinct loci. Complete copies of putative APN1 (DNA lyase) and SLA2 (cytoskeleton assembly control) genes were found bordering the MAT1 locus. Partial copies of APN1 and SLA2 were also found bordering the MAT2 locus, but these copies bore the genetic hallmarks of pseudogenes. Genome comparisons revealed synteny over at least 23,300 bp between the N. fischeri MAT1 region and the A. fumigatus MAT locus region, but no such long-range conservation in the N. fischeri MAT2 region was evident. The sequence upstream of MAT2 contained numerous candidate transposase genes. These results demonstrate a novel means involving the segmental translocation of a chromosomal region by which the ability to undergo self-fertilization may be acquired. The results are also discussed in relation to their significance in indicating that heterothallism may be ancestral within the Aspergillus section Fumigati.

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Section: Analysis Of N Fischeri Mat1-and Mat2-flanking Regions Blasmentioning

confidence: 99%

Section: Analysis Of N Fischeri Mat1-and Mat2-flanking Regions Blasmentioning

confidence: 99%

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confidence: 99%

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DNA Sequence Characterization and Molecular Evolution of MAT1 and MAT2 Mating-Type Loci of the Self-Compatible Ascomycete Mold Neosartorya fischeri

2007

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“…Fingerprinting techniques (RAPD-PCR) applied to single spore isolates derived from single asci (i.e., the progeny of meiosis) and to the sterile cultured vegetative mycelium of the ascocarp of X. parietina revealed identical fingerprints and, thus, it was concluded to be homothallic by Honegger et al (2004a). The genetic background was elucidated in molecular analyses of the mating type loci (MAT1-1-1 and MAT1-2-1) plus flanking regions of a range of Xanthoria species, where the homothallic X. parietina was shown to contain MAT 1-2-1 in all descendants of meiosis, MAT 1-1-1 having been lost (Scherrer et al 2005; nomenclature according to Turgeon & Yoder 2000); thus recombination seems unlikely to occur in X. parietina. Xanthoria elegans, another homothallic species, had MAT1-1-1 and MAT1-2-1 in all siblings (Scherrer et al 2005).…”

Section: Introductionmentioning

confidence: 99%

“…The genetic background was elucidated in molecular analyses of the mating type loci (MAT1-1-1 and MAT1-2-1) plus flanking regions of a range of Xanthoria species, where the homothallic X. parietina was shown to contain MAT 1-2-1 in all descendants of meiosis, MAT 1-1-1 having been lost (Scherrer et al 2005; nomenclature according to Turgeon & Yoder 2000); thus recombination seems unlikely to occur in X. parietina. Xanthoria elegans, another homothallic species, had MAT1-1-1 and MAT1-2-1 in all siblings (Scherrer et al 2005). Heterothallic species such as X. calcicola, X. polycarpa or X. capensis have either MAT1-1-1 or MAT1-2-1 in half of the siblings derived from one ascus and polymorphic fingerprints (2 × 4 genotypes among the 8 sporelings per ascus) resulting from successful recombination (Honegger et al 2004a;Scherrer et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Population genetics in the homothallic lichen-forming ascomyceteXanthoria parietina

Itten¹,

Honegger²

2010

The Lichenologist

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Abstract:The genetic diversity within and among populations of Xanthoria parietina was studied at the subspecific level with a fingerprinting technique (RAPD-PCR) applied to sterile cultured multispore isolates, each being derived from a single apothecium. Populations from coastal, rural and urban sites from NW, SW and central France and from NE Switzerland were investigated. Between 1 and 8 microsites of a few decimetres square, each comprising 13 to 27 thalli of X. parietina, were analysed per population. A total of 132 isolates from epiphytic and 3 isolates from epilithic specimens were investigated. Phenotypic variation was recorded among some of the thalli in the field and among sterile cultured isolates in the laboratory. A high diversity of genotypes was observed, even among thalli growing side by side in phenotypically homogenous populations. An average of 73·5 % polymorphism was found in all samples. As shown with Principal Coordinates Analysis (PCO), most of the genetic variation (90%) resided within, not among, populations. As X. parietina had previously been shown with molecular and fingerprinting techniques to be homothallic, the potential genetic background of this diversity is discussed. Intense genotype rather than gene (allele) flow seems to be an important element in X. parietina populations.

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Lichen Genomics

Grube

Berg

Andrésson³

et al. 2013

The Ecological Genomics of Fungi

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Characterisation of the mating-type locus in the genus Xanthoria (lichen-forming ascomycetes, Lecanoromycetes)

Cited by 44 publications

References 42 publications

DNA Sequence Characterization and Molecular Evolution of MAT1 and MAT2 Mating-Type Loci of the Self-Compatible Ascomycete Mold Neosartorya fischeri

DNA Sequence Characterization and Molecular Evolution of MAT1 and MAT2 Mating-Type Loci of the Self-Compatible Ascomycete Mold Neosartorya fischeri

Population genetics in the homothallic lichen-forming ascomyceteXanthoria parietina

Lichen Genomics

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