Characterisation of some<i>Phlebiopsis gigantea</i>isolates with respect to enzymatic activity and decay of Norway spruce wood

Żółciak, Anna; Sierota, Z.; Małecka, Monika

doi:10.1080/09583157.2012.691156

Cited by 15 publications

(20 citation statements)

References 26 publications

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“…After six months of cultivation, samples b were cleaned from aerial mycelium only, dried and weighed. The loss of dry mass of wood was counted and compared with initial dry weight [17].…”

Section: Wood Samplesmentioning

confidence: 99%

“…In Poland, some of the issues concerning challenges of successful treatment have already been solved. The results obtained by Żółciak et al [16,17] allowed selecting P. gigantea strains, including registered ones, with enhanced potential to produce specific enzymes to fast decay pine and spruce wood under laboratory conditions. Activities of cellulase and peroxidase seem to be an important factor in selecting P. gigantean strains as roots and stumps wood decomposer in commercial preparations.…”

mentioning

confidence: 99%

“…Żółciak et al [17] found that the production of cellulase by several P. gigantea strains was higher when compared to other isolates tested and generally greater in the wood mycelium than that in the aerial mycelium.…”

mentioning

confidence: 99%

“…Specificity of forest location, environmental conditions as well as resultant differentiation in genetic and physiological features of coniferous stands in Poland encourage undertaking studies on P. gigantea strains effectiveness and their practical usefulness at a local level, in view of different climatic, habitat and management conditions [17,24]. Since it is possible that the registered strains (active in tests performed in the country of their origin) can show the other kind of activity under different habitat conditions in another country.…”

mentioning

confidence: 99%

“…It was assumed that the results of molecular analyses carried out with the use of random amplified microsatellite (RAMS) method [27][28][29][30][31] as well as comparisons of strain enzymatic, and wood decay activities would distinguish similarities between the strains tested and point towards the most vigorous ones, in view of treatment efficacy in practice. The results of some enzyme assays and Norway spruce wood decay assessments described below were extracted from the database described by Żółciak et al [17].…”

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confidence: 99%

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What Is Important in Selecting Phlebiopsis gigantea Strain for Commercial Use?

Sierota¹,

Sikora²,

Żółciak³

2015

JAST-B

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Phlebiopsis gigantea strains registered in the European Union as biocontrol agents against root rot in forests (four from Great Britain and two from Finland) were compared using Ward's method with reference to: (1) similarity of DNA-random amplified microsatellite (RAMS) ladders, (2) cellulase and peroxidase production and (3) acceleration of dry mass wood loss in Norway spruce. The activity of the enzymes was tested in the initial phase of wood decay (30 d after inoculation) and indicated as the most active isolates: VF10 and FC15 for cellulase and FC16 and VF10 for peroxidase production. The assessment of loss of wood six months after inoculation indicated isolate FC15 as the most active. P. gigantea isolates similar in terms of enzyme activity indicated different patterns of DNA microsatellite loci. At the same time, DNA-RAMS revealed similarities in isolates with different abilities to produce enzymes. However, some similarities and differences between isolates according to wood decay were found. No plain relationships between molecular characteristics and enzyme activity of the strains tested were observed. The results differentiated activity of tested isolates and suggested benefits of selecting P. gigantea strains for commercial use basing mainly on the assessment of wood loss activity.

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“…After six months of cultivation, samples b were cleaned from aerial mycelium only, dried and weighed. The loss of dry mass of wood was counted and compared with initial dry weight [17].…”

Section: Wood Samplesmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

What Is Important in Selecting Phlebiopsis gigantea Strain for Commercial Use?

Sierota¹,

Sikora²,

Żółciak³

2015

JAST-B

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Host-associated Intraspecific Phenotypic Variation in the Saprobic Fungus Phlebiopsis gigantea

et al. 2023

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Whether intraspecific phenotypic variation in saprobic fungi may be driven by the host of origin has received little attention. We addressed this issue by testing hypotheses using the model system Phlebiopsis gigantea, a wood destroying fungus associated with Picea abies and Pinus sylvestris, among others, and widely employed in practical forestry as a biological control agent. By examining approximately 60 sympatric P. gigantea isolates from both P. abies and P. sylvestris, we showed that the former grew in vitro significantly (P < 0.05) slower than the latter (average 5.56 mm/day vs. 6.84) while producing 1.8-fold significantly higher number of mitospores. An overall significant trade-off between these two phenotypic traits was detected, in particular for isolates originating from P. abies. Comparative inoculation experiments of a subsample of isolates and the assessment of mycelial growth in logs of both hosts allowed to reject the hypothesis that isolates are equally fit in terms of growth rate in wood of both hosts regardless of the host of origin. Tree models revealed that the growth rate of isolates was associated not only with the wood species in which the isolates were inoculated (P < 0.001), P. sylvestris being more rapidly colonized than P. abies, but also with the host of origin of isolates (P < 0.001). Results showed that P. gigantea isolates originating from different hosts differ phenotypically in terms of some key phenotypic traits demonstrating that a host-driven intraspecific phenotypic variation may occur in saprobic fungi.

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Interspecific interactions within fungal communities associated with wood decay and forest trees

Awan

Asiegbu

2021

Forest Microbiology

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Characterisation of somePhlebiopsis giganteaisolates with respect to enzymatic activity and decay of Norway spruce wood

Cited by 15 publications

References 26 publications

What Is Important in Selecting Phlebiopsis gigantea Strain for Commercial Use?

What Is Important in Selecting Phlebiopsis gigantea Strain for Commercial Use?

Host-associated Intraspecific Phenotypic Variation in the Saprobic Fungus Phlebiopsis gigantea

Interspecific interactions within fungal communities associated with wood decay and forest trees

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