Characterisation of soluble and insoluble cell wall fractions from rye, wheat and hull-less barley endosperm flours

Abstract

Please cite this article as: Comino, P., Collins, H., Lahnstein, J., Beahan, C., Gidley, M.J, Characterisation of soluble and insoluble cell wall fractions from rye, wheat and hull-less barley endosperm flours, Food Hydrocolloids (2014), doi: 10.1016/j.foodhyd.2014.04.005. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Sequential fractionation of feruloylated hemicelluloses and oligosaccharides from wheat bran using subcritical water and xylanolytic enzymes

“…3,29,31,32 The total hydroxycinnamic acid content was 2.2-2.4 mg g −1 , with ferulic acid (FA) being the dominating compound. Similar or lower amounts of FA were reported for insoluble cell wall fractions of wheat endosperm 33 and extracted AXs from wheat bran. 8,34 Phytic acid (9.6 ± 0.5 mg g −1 ) and acetyl content (degree of acetylation, DA = 0.03) showed lower values than previously reported for wheat bran.…”

“…39 f Hydroxycinnamic acid content determined by GC-MS. 33 g Klason lignin after acid hydrolysis (H 2 SO 4 ). h Determined based on the difference between the sum of total carbohydrate, protein, phenolic and lignin and the initial dry weight.…”

Microstructure and mechanical properties of arabinoxylan and (1,3;1,4)-β-glucan gels produced by cryo-gelation

“…The phenolic content of the extracted arabinoxylan was determined following the method described elsewhere (Comino, Collins, Lahnstein, Beahan & Gidley, 2014). Samples were resuspended in dichloromethane (DCM) before injection onto a GC-MS fitted with a CP SIL 5 column (Agilent Technologies Australia Pty Ltd; Mulgrave, Victoria, Australia).…”

“…2.6 Immunolabelling/confocal laser scanning microscopy A 2 mm slice was cut from the freeze dried cryogels and half of it immunolabelled following the method by Comino et al (Comino, Collins, Lahnstein, Beahan & Gidley, 2014) with some modifications. Labelling was performed in Eppendorf tubes.…”

“…Firstly, we pre-form cross-linked networks of arabinoxylan as the current evidence suggests that this is relevant in vivo (Comino, Collins, Lahnstein, Beahan & Gidley, 2014;Saulnier, Sado, Branlard, Charmet & Guillon, 2007). Secondly we induce polymer concentration by cryo-gelation, which forces polymers together by their exclusion from ice crystals in a very approximate mimic of the concentration achieved by extracellular deposition of polysaccharides from adjacent turgid cells.…”