“…Port Salut Argentino is a semi-cooked cheese, produced from pasteurised milk, acidified by lactic bacteria, coagulated by rennet and/or other specific enzymes, salted by immersion in brine, and ripened for a short period (Co´digo Alimentario Argentino, 1981). We have recently characterised the proteolysis of Port Salut Argentino cheese using chromatographic profiles of different nitrogenous fractions that allowed the separation of several components including a s1 -casein, a s1 -I-casein, water-soluble fragments (including a s1 -CN (f1-23)), and free amino acids (Verdini, Zorrilla, & Rubiolo, 2004). Earlier studies (Verdini, Zorrilla, & Rubiolo, 2002) found that the freezing process increased the content of water-soluble nitrogen and total free amino acids during the ripening of Port Salut Argentino cheeses frozen prior to ripening.…”