Characterisation of sensitivity of Colletotrichum gloeosporioides and Colletotrichum capsici, causing pepper anthracnose, to picoxystrobin

Ren, Lixuan; Wang, S. F.; Shi, Xiaojing; Cao, Junli; Zhou, Jianbo; Zhao, Xinbei

doi:10.1007/s41348-020-00316-y

Cited by 14 publications

(9 citation statements)

References 34 publications

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“…However, as a group of fungicides with a single action site, it is much easier to develop QoI‐resistant mutants in the laboratory and field. Picoxystrobin, a QoI fungicide, has shown great activity against Colletotrichum capsici and C. gloeosporioides in vitro and good efficacy against banana leaf spot disease and chili anthracnose in the field 29,30,36 . However, no information on the antifungal activity of picoxystrobin against M. oryzae is available.…”

Section: Discussionmentioning

confidence: 99%

“…gloeosporioides in vitro and good efficacy against banana leaf spot disease and chili anthracnose in the field. 29,30,36 However, no information on the antifungal activity of picoxystrobin against M. oryzae is available.…”

Section: Discussionmentioning

confidence: 99%

“…Picoxystrobin is a QoI fungicide which is mainly used in a combination with some fungicides from other chemical groups to control diseases in the field and little information about the activity and control efficacy of picoxystrobin has been reported. 29,30 However, it has not been registered for the control of rice blast in China. Therefore, no information is available about its activity on M. oryzae or the resistance risk of picoxystrobin in China.…”

Section: Introductionmentioning

confidence: 99%

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The G143A/S substitution of mitochondrially encoded cytochrome b (Cytb) in Magnaporthe oryzae confers resistance to quinone outside inhibitors

Gao

et al. 2022

Pest Management Science

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BACKGROUND: Rice blast, caused by Magnaporthe oryzae, is a destructive disease threatening the production of staple foods worldwide. Quinone outside inhibitors (QoIs) are a group of chemicals exhibiting excellent activity against a majority of plant pathogens, with the disadvantage that pathogens can easily develop resistance to QoIs.RESULTS: Here, we investigated the activity of picoxystrobin against M. oryzae, which showed a great inhibitory effect on 100 strains of M. oryzae with half-maximal effective concentrations (EC 50 ) ranging from 0.0251 to 0.1337 ∼g ml −1 . The EC 50 values showed a continuous unimodal distribution that was identical to the normal distribution, suggesting the potency of our study to represent baseline sensitivity. In addition, nine resistant mutants were obtained by exposing M. oryzae to a high dosage of picoxystrobin in the laboratory; all of them showed cross-resistance to the other five QoI fungicides. Although some mutants showed a decreased resistance factor after ten successive cultures on fungicide-free medium, the resistance to picoxystrobin was still inheritable. Amino acid substitution of G143S was detected in eight of nine picoxystrobin-resistant mutants, and G143A was detected in only one of nine mutants. A fitness penalty was found in the mutants carrying G143S rather than G143A.CONCLUSION: Our findings suggested that M. oryzae had a mid to high risk of resistance to picoxystrobin. Considering this, we should be vigilant to the resistance risk and apply picoxystrobin sensibly in the field.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The G143A/S substitution of mitochondrially encoded cytochrome b (Cytb) in Magnaporthe oryzae confers resistance to quinone outside inhibitors

Gao

et al. 2022

Pest Management Science

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“…Others have shown that Cytb gene mutation was not also detected in few pathogens, such as reduced sensitivity to azoxystrobin L . theobromae isolates [ 34 ], low and moderate resistant Colletotrichum gloeosporioides isolates [ 35 ], moderate and high resistant Microdochium majus isolates [ 36 ]. Though L .…”

Section: Discussionmentioning

confidence: 99%

“…The QoI fungicides resistance generally associated with point mutations in Cytb gene at codons 129,137 and 143 in many phytopathogens [12,13,33]. Others have shown that Cytb gene mutation was not also detected in few pathogens, such as reduced sensitivity to azoxystrobin L. theobromae isolates [34], low and moderate resistant Colletotrichum gloeosporioides isolates [35], moderate and high resistant Microdochium majus isolates [36]. Though L. theobromae from mango has high level resistance to pyraclostrobin, but no point mutation was detected in pyraclostrobin-resistant isolates in this study.…”

Section: Plos Onementioning

confidence: 99%

Resistance mechanisms and fitness of pyraclostrobin-resistant isolates of Lasiodiplodia theobromae from mango orchards

Yang

et al. 2021

PLoS ONE

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Background Stem-end rot, caused by Lasiodiplodia theobromae (Pat.) Griffon & Maubl is a serious postharvest disease in mango. In China, a high prevalence of the QoI fungicides resistance has been reported in the last decade. The study aimed to discuss factors determining rapid development of pyraclostrobin-resistance and its resistance mechanisms. Methods To determine the resistance stability and fitness of pyraclostrobin resistance in L. theobromae, three phenotypes of pyraclostrobin resistance were compared and analyzed for the EC50 values, mycelial growth, virulence and temperature sensitivity and osmotic stress sensitivity. The relative conductivity and enzyme activities of different phenotypes were compared under fungicide stress to explore possible biochemical mechanisms of pyraclostrobin resistance in L. theobromae. The Cytb gene sequences of different phenotypes were analysed. Results All isolates retained their original resistance phenotypes during the 10 subcultures on a fungicide-free PDA, factor of sensitivity change (FSC) was approximately equal to 1. The resistance-pyraclostrobin of the field isolates should be relatively stable. Two pyraclostrobin-resistant phenotypes shared similar mycelial growth, virulence and temperature sensitivity with pyraclostrobin-sensitive phenotype. After treated by pyraclostrobin, the relative conductivity of the sensitive phenotype was significantly increased. The time of Pyr-R and Pyr-HR reached the most conductivity was about 8–10 times than that of Pyr-S, the time for the maximum value appearance showed significant differences between sensitive and resistant phenotypes. The activities of Glutathione S-transferase (GST), catalase (CAT) and peroxidase (POD) of Pyr-HR were 1.78, 5.45 and 1.65 times respectively, significantly higher than that of Pyr-S after treated by 200 mg/l pyraclostrobin. Conclusion The results showed that the pyraclostrobin-resistant phenotypes displayed high fitness and high-risk. The nucleotide sequences were identical among all pyraclostrobin-resistant and -sensitive isolates. The pyraclostrobin resistance was not attributable to Cytb gene alterations, there may be some of other resistance mechanisms. Differential response of enzyme activity and cell membrane permeability were observed in resistant- and sensitive-isolates suggesting a mechanism of metabolic resistance.

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Biochemical and genetic characterization of Botrytis cinerea laboratory mutants resistant to propamidine

Zhang

Huang

Zhang

et al. 2022

Pest Management Science

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BACKGROUND Botrytis cinerea, the causal agent of gray mold, is one of the top 10 fungal pathogens in the world. Propamidine, an aromatic diamidine compound, exhibited both protective and therapeutic effects against B. cinerea. However, the resistance risk and mechanism of B. cinerea to propamidine are unclear. RESULTS Twelve high and stable resistant mutants were obtained from B. cinerea B05.10 by fungicide induction. Compared with the parental strain, the biological fitness of the mutants, including growth rate, spore germination, pathogenicity, and oxalic acid decreased significantly. There was no cross‐resistance among propamidine and other commonly used fungicides, while the efficacy of propamidine against the resistance mutants declined. In addition, the cell membrane permeability, substance metabolism, and defense enzyme activities of the resistant mutants were significantly increased compared with the wild strain. Whole‐genome sequencing of all resistant mutants found that there were 32 SNPs and nine InDels. Importantly, nine common single‐point mutant genes in the exon region were found in all 12 resistant mutants, and these genes were related to multiple pathways in vivo, indicating that many factors contributed to the formation of propamidine resistance. CONCLUSION These data suggested the resistance risk of B. cinerea to propamidine was low to moderate and the mechanism of propamidine was different from that of the existing fungicides. These results will increase understanding of the resistance mechanism of propamidine and provide a critical basis for the rational design of pesticide molecules based on targets. © 2022 Society of Chemical Industry.

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Characterisation of sensitivity of Colletotrichum gloeosporioides and Colletotrichum capsici, causing pepper anthracnose, to picoxystrobin

Cited by 14 publications

References 34 publications

The G143A/S substitution of mitochondrially encoded cytochrome b (Cytb) in Magnaporthe oryzae confers resistance to quinone outside inhibitors

The G143A/S substitution of mitochondrially encoded cytochrome b (Cytb) in Magnaporthe oryzae confers resistance to quinone outside inhibitors

Resistance mechanisms and fitness of pyraclostrobin-resistant isolates of Lasiodiplodia theobromae from mango orchards

Biochemical and genetic characterization of Botrytis cinerea laboratory mutants resistant to propamidine

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