Characterisation of reversed-phase liquid chromatographic columns by chromatographic testsRational column classification by a minimal number of column test parameters

Visky, D.; Heyden, Yvan Vander; Iványi, Tímea; Baten, P.; Beer, Jacques O. De; Kovács, Zsuzsanna; Noszál, Béla; Dehouck, Pieter; Roets, Eugène; Massart, D.L.; Hoogmartens, Jos

doi:10.1016/s0021-9673(03)01142-7

Cited by 68 publications

(25 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Multivariate techniques (principal component analysis, factor analysis, cluster analysis etc.) are widely applied for selection of proper stationary phase and/or preferred solvents [7][8][9][10][11]. Principal component analysis (PCA) is often used for classification of columns.…”

Section: Introductionmentioning

confidence: 99%

“…For example, Visky et al characterized 69 reversed-phase LC columns on the basis of 24 measured chromatographic parameters using eight different chromatographic methods and evaluated the data by PCA. The best parameter set utilized only four parameters of these: k 0 amylbenzene , r benzylamine, phenol , k 0 2,2-dipyridyl and r triphenylene, o-terphenyl [8]. The results of the column tests were correlated with the quality of separation of aromatic acids characterized by chromatographic response function (CRF) as the optimization criterion [9].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of HPLC columns for two‐dimensional LC × LC separations of phenolic acids and flavonoids

Jandera

Vyňuchalová

Hájek

et al. 2008

Journal of Chemometrics

View full text Add to dashboard Cite

The aim of the work is to contribute to rational selection of columns and mobile phases for 'orthogonal' twodimensional LC T LC operation of specific sample types, providing maximum increase in the peak capacity with respect to single-dimension HPLC. As a criterion of suitability, the dissimilarity of the separation selectivity of the phase separation systems was selected. For this purpose, correlations between the retention and molecular structure descriptors were used to characterize and compare the columns with respect to various types of selective interactions using the interaction indices as the general polarity scale characteristics and the linear free energy relationships (LFER) model employing selective molecular structural descriptors. Chemometric methods-cluster analysis using similarity dendrograms and multiple regression analysis-were used to classify several types of bonded C18, amide and polyethyleneglycol columns on the basis of differences in separation selectivities for selected phenolic and flavone standards. Systematic development of a comprehensive LC T LC method for two-dimensional separation of phenolic acids and flavone natural antioxidants was based on the selection of the combination of first-and second-dimension columns with low correlation of retention of representative standards providing a highly orthogonal two-dimensional separation system. The mobile phases providing best separation selectivity for phenolic compounds on each column tested were optimized using window-diagram optimization strategy. The final optimized conditions were employed for comprehensive LC T LC separations of natural antioxidants using a polyethylene glycol microcolumn in the first dimension and a short monolithic C18 column in the second dimension.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of HPLC columns for two‐dimensional LC × LC separations of phenolic acids and flavonoids

Jandera

Vyňuchalová

Hájek

et al. 2008

Journal of Chemometrics

View full text Add to dashboard Cite

show abstract

“…3,10 To estimate the silanol effects in practical ODS columns, numerous tests have been proposed to evaluate the silanol activity on the surface of silica-based ODS (octadecylsilyl) packing materials. 5,7,[11][12][13][14][15][16][17][18][19][20][21][22] Most of the probe compounds used for evaluating the silanol activity were small molecules containing nitrogen atoms, such as p-ethylaniline, 17,18 caffeine, 19 benzylamine, 19 aniline, 20 and so on. 23 Nitrogen atoms in these probe compounds interact with silanol groups through the ionexchange or hydrogen-bonding ability of the silanol groups.…”

Section: Introductionmentioning

confidence: 99%

Tris(2-methyl-8-quinolinolato)iron(III) as a Novel Spectrophotometric Probe for Silanol Detection

2006

View full text Add to dashboard Cite

Tris(2-methyl-8-quinolinolato)iron(III) was proposed as a sensitive spectrophotometric silanol-detecting probe based on the coordination ability of silanol groups on the surface of octadecylsylanized silica gel (ODS silica gel) for reversedphase high-performance liquid chromatography. A peak of the iron(III) complex on a chromatogram abruptly collapsed as the silanol content in an ODS column increased, indicating that the iron(III) complex could sense trace amounts of silanol groups. The change of the peak parameters, such as the peak height and the peak area was highly related to the output of some nitrogen-containing compounds used as silanol-detecting probes as a function of the silanol content in an ODS column. The response of the peak height of the iron(III) complex to the silanol content was much more sensitive than the response of the nitrogen-containing probes, and was comparable to that of tris(2-methyl-8-quinolinolato)gallium(III), which had been proposed as a fluorometric silanol-detecting probe based on the coordination ability of the silanol groups.

show abstract

“…The influences of the residual silanol groups, so-called silanol effects, have drawn much attention, and numerous probe compounds have been proposed to evaluate the silanol activity on the surface of silicabased ODS (octadecylsilyl) packing materials. 5,7,[10][11][12][13][14][15][16] Most of the probe compounds are small molecules containing nitrogen atoms, because much interest has been focused on the interaction through the ion-exchange or hydrogen-bonding function to which nitrogen-containing compounds are considered to respond. 10,[17][18][19][20][21][22][23] Claessens et al 16,24,25 examined the relations between various silanol-detecting molecules containing nitrogen atoms, and found a correlation among the results of the tests.…”

mentioning

confidence: 99%

Tris(2-methyl-8-quinolinolato)gallium(III) as a Fluorescent Probe for Sensitive Silanol-Testing

2005

View full text Add to dashboard Cite

Characterisation of reversed-phase liquid chromatographic columns by chromatographic testsRational column classification by a minimal number of column test parameters

Cited by 68 publications

References 29 publications

Characterization of HPLC columns for two‐dimensional LC × LC separations of phenolic acids and flavonoids

Characterization of HPLC columns for two‐dimensional LC × LC separations of phenolic acids and flavonoids

Tris(2-methyl-8-quinolinolato)iron(III) as a Novel Spectrophotometric Probe for Silanol Detection

Tris(2-methyl-8-quinolinolato)gallium(III) as a Fluorescent Probe for Sensitive Silanol-Testing

Contact Info

Product

Resources

About