Characterisation of rapid alkalinisation factors in <i>Physcomitrium patens</i> reveals functional conservation in tip growth

Ginanjar, Eggie Febrianto; Teh, Ooi-Kock; Fujita, Tomomichi

doi:10.1111/nph.17942

Cited by 16 publications

(37 citation statements)

References 93 publications

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“…PpRALFs were clustered with AtRALFs that inhibit elicitor-induced ROS production (Abarca et al, 2021). These results are consistent with data of previous phylogenetic analysis (Ginanjar et al, 2022), where PpRALFs were grouped with AtRALF23, AtRALF33, AtRALF23, AtRALF22. These findings suggest that overall amino acid composition of RALF peptides, to some extent, reflect their functional biases and PpRALFs are related to AtRALFs that negatively regulate immune response.…”

Section: Resultssupporting

confidence: 93%

“…According to our data, the PpRALF1 peptide has no role in stress response, but the knockouts of two PpRALFs (2 and 3) resulted in increasing resistance to bacterial and fungal pathogens - P. carotovorum and F. solani , suggesting the negative regulation of immune response by these peptides. These PpRALFs are also placed in a distinct group on the phylogenetic tree (Ginanjar et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

“…The function of RALF genes that have been identified in bryophyte genomes are still being studied. PpRALF1 and PpRALF2 - out of three PpRALF peptides identified in P. patens have been shown to promote protonema tip growth and elongation (Ginanjar et al, 2022). Although, the function of PpRALF3 peptide is still unknown.…”

Section: Introductionmentioning

confidence: 99%

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RALF peptides modulate immune response in the mossPhyscomitrium patens

Mamaeva

Lyapina

Knyazev

et al. 2022

Preprint

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RAPID ALKALINIZATION FACTOR (RALFs) are cysteine-rich peptides that regulate multiple physiological processes in plants. This peptide family has considerably expanded during land plant evolution, but the role of ancient RALFs in modulating stress response is unknown. Here, we used the mossPhyscomitrium patensas a model to gain insight into the role of RALF peptides in coordination of plant growth and stress response in non-vascular plants. The quantitative proteomic analysis revealed concerted downregulation of M6 metalloproteases and some membrane proteins, including those involved in stress response, inPpRALF1, 2and3knockout (KO) lines. We found that knockout ofPpRALF2andPpRALF3genes resulted in increased resistance to bacterial and fungal phytopathogens -Pectobacterium carotovorumandFusarium solani, suggesting the role of these peptides in negative regulation of immune response inP. patens. The comparative transcriptome analysis ofPpRALF3KO and wild type plants underFusarium solaniinfection showed the clear difference in regulation of genes belonging to phenylpropanoid pathway and associated with cell wall modification and biogenesis between these genotypes. The follow-up analysis revealed the role of PpRALF3 in growth regulation under abiotic and biotic stress regulation, which suggests the role of RALFs in responses to different adverse conditions. Thus, our study sheds light on the function of the previously uncharacterized PpRALF3 peptide and gives a clue to ancestral functions of RALF peptides in plant stress response.

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Section: Resultssupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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RALF peptides modulate immune response in the mossPhyscomitrium patens

Mamaeva

Lyapina

Knyazev

et al. 2022

Preprint

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“…Moreover, no RALF orthologs have been reported in algae, suggesting the origin of the CrRLK1L pathway in land plants [48]. A similar conclusion was recently reached by analyzing the RALF family in P. patens [63]. Moreover, the best candidate obtained by BLAST analysis using the MpFER malectin-like domain against the genome of the Charophycean alga Chara braunii clustered outside the CrRLK1L phylogenetic tree (Figs.…”

Section: Crrlk1l Is Conserved In Land Plants and Arose In This Lineagesupporting

confidence: 69%

The singleMarchantia polymorpha FERONIAhomolog reveals an ancestral role in regulating cellular expansion and integrity

Mecchia

Rövekamp

Giraldo-Fonseca

et al. 2020

Preprint

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SummaryPlant cells are surrounded by a cell wall, a rigid structure rich in polysaccharides and glycoproteins. The cell wall is not only important for cell and organ shape, but crucial for intercellular communication, plant-microbe interactions, and as a barrier to the environment. In the flowering plant Arabidopsis thaliana, the 17 members of the Catharanthus roseus RLK1-like (CrRLK1L) receptor kinase subfamily are involved in a multitude of physiological and developmental processes involving the cell wall, including reproduction, hormone signaling, cell expansion, innate immunity, and various stress responses. Due to genetic redundancy and the fact that individual CrRLK1Ls can have distinct and sometimes opposing functions, it is difficult to assess the primary or ancestral function of CrRLK1Ls. To reduce genetic complexity, we characterized the single CrRLK1L gene of Marchantia polymorpha, MpFERONIA (MpFER). Plants with reduced MpFER levels show defects in vegetative development, i.e., rhizoid formation and cell expansion, but also affect male fertility. In contrast, Mpfer null mutants and overexpression lines severely affect cell integrity and morphogenesis of the gametophyte. Thus, the CrRLK1L gene family originated from a single gene with an ancestral function in cell expansion and the maintenance of cellular integrity. During land plant evolution, this ancestral gene diversified and was recruited to fulfil a multitude of specialized physiological and developmental and roles in the formation of both gametophytic and sporophytic structures essential to the life cycle of flowering plants.

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“…However, pollen tubes can undergo tip growth without attractants in vitro , suggesting that the mechanism underlying tip growth is mostly intrinsic. In mosses, three RALF‐encoding genes have been characterized and shown to regulate tip growth (Ginanjar et al ., 2022). Interestingly, PpRALF1 and PpRALF2 are secreted but differently localized.…”

Section: Receptor‐like Kinasesmentioning

confidence: 99%

ROP GTPase‐dependent polarity establishment during tip growth in plants

2022

New Phytologist

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Summary Polar cell growth in plants requires a cell peripheral region that undergoes membrane extension and cell wall remodeling. Since the 1990s, RHO‐RELATED GTPASES FROM PLANTS (ROPs) have been identified as master regulators that determine the site of cell growth. ROPs function to regulate actin and microtubule cytoskeletons, calcium gradients, and exocytosis, thus directing the delivery of materials for membrane and cell wall extension. In recent years, our understanding of the regulatory mechanisms underlying polar localization and the activation of ROPs has greatly advanced. Evidence points to the crucial roles of membrane lipids, receptor‐like kinases, and cell wall components. In this review, we provide updates on the mechanisms underlying polarity control in tip‐growing cells, with a focus on ROP effectors and membrane‐associated signals. By integrating knowledge from pollen tubes, root hairs, and findings in bryophyte protonema cells and rhizoids, we hope to offer important insights into a common conceptual framework on polarity establishment governed by intercellular and extracellular signals.

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Characterisation of rapid alkalinisation factors in Physcomitrium patens reveals functional conservation in tip growth

Cited by 16 publications

References 93 publications

RALF peptides modulate immune response in the mossPhyscomitrium patens

RALF peptides modulate immune response in the mossPhyscomitrium patens

The singleMarchantia polymorpha FERONIAhomolog reveals an ancestral role in regulating cellular expansion and integrity

ROP GTPase‐dependent polarity establishment during tip growth in plants

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