Characterisation of quaternary mixtures by the apparent content curves method: identification of tocopherols in vegetable oils

Abstract

A procedure for identification of the compounds in quaternary mixtures has been developed. The proposed procedure is based on the apparent content curves method. From these curves and using the Q parameter, work conditions are selected and quaternary mixtures treated as "pseudoternary" mixtures.A simple strategy to test matrix effects at working wavelengths has been developed, identification limits established and following the identification table quaternary mixtures were easily characterised. The procedure has been applied to the identification of phenols in mixtures by UV-visible spectrophotometry and tocopherols in edible oils by fluorimetry. Results obtained for edible oils are in agreement with the composition obtained by use of the reference method.

Utilization of Synchronous Fluorescence Spectroscopy to Detect Adulteration of Olive Oil

“…Różnice w widmach fluorescencyjnych poszczególnych olejów, uzyskane w danych warunkach pomiaru, wynikają ze zróżnicowania ilościowo-jakościowego składu związków wykazujących naturalną emisję, takich jak: tokoferole, chlorofile oraz związki fenolowe [5,9,16,17].…”

Comparison of Different Fluorimetric Signals for the Simultaneous Multivariate Determination of Tocopherols in Vegetable Oils

“…The quaternary mixture of tocopherols in edible oils has been characterized by fluorimetry by using the apparent content curves method (ACC). 32 The ACC method is applied to the synchronous spectra of tocopherols in hexane and in isopropanol. It was observed that the excitation spectra are different for the different tocopherols, while the emission spectra have the same shape.…”