Characterisation of proteins from date palm sap (Phoenix dactylifera L.) by a proteomic approach

Thabet, Imène Ben; Francis, Frédéric; Pauw, Edwin De; Besbes, Souhail; Attia, Hamadi; Deroanne, Claude; Blecker, Christophe

doi:10.1016/j.foodchem.2010.05.024

Cited by 18 publications

(15 citation statements)

References 30 publications

(32 reference statements)

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“…The previously described results and unique proteomic study on date palm sap (Ben Thabet et al, 2010b) confirm that this protein (30 kDa) was a plant protein of tapping, this content increased (p< 0.05) in the male sap. Similar to most plants, the free amino acid pool of both male and female saps (Table 3) was dominated by glutamine, asparagine (Fischer et al, 1998), and arginine (Salaün and Charpentier, 2001).…”

Section: Electrophoretic Profile Of Date Palm Sapsupporting

confidence: 78%

“…This result could presumably be ascribed to glucose -6-phosphate isomerase activity, a non rate-limiting enzyme in glycolysis, which catalyses the reversible isomerisation of glucose-6-phosphate to fructose-6-phosphate (Haller et al, 2011). Such invertase and glucose-6-phosphate isomerase activities could be attributed to the proteins involved in glycolysis and related to Saccharomyces cerevisiae which belongs to the natural microflora of date palm sap (Ben Thabet et al, 2010b).…”

Section: Sugar Profilementioning

confidence: 99%

“…In addition, proteins that this yeast involves against stress such as ATPase of Hsp90 chaperone complex, present a molecular weight nearly 77.6 kDa. The metabolism proteins produced by Saccharomyces cerevisiae, like Acetohydroxyacid reductoisomerase, were also identified with a molecular weight of about 45 kDa (Ben Thabet et al, 2010b). Overall, the collection time affects the quality of Deglet Nour date palm sap (Phoenix dactylifera L.).…”

Section: Electrophoretic Profile Of Date Palm Sapmentioning

confidence: 99%

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Physico-chemical properties and amino acid profiles of sap from Tunisian date palm

Makhlouf-Gafsi

Mokni-Ghribi

Bchir

et al. 2016

Sci. agric. (Piracicaba, Braz.)

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Date palm sap (Phoenix dactylifera L.), also known as "legmi", is a fresh juice extracted from date palm trees. The present study aimed to elucidate the effects of collection time (at the beginning of the tapping period and after seven days of collection) on the amino acid profile and physico-chemical properties of date palm sap from both male and female trees.Dry matter, protein, amino acid, and sugar profiles were determined using the Kjeldahl method, High-Performance Liquid Chromatography (HPLC), and High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD), respectively. Date palm sap from both male and female trees contained high levels of carbohydrates. HPLC analysis showed that this fraction was dominated by sucrose in the sap sample from female trees compared to that from male trees. Male date palm sap was noted to exhibit lower dry matter content than female date palm sap but higher protein, total polyphenol, ash, and amino acid contents. While the major essential amino acids in the sap from male trees consisted of valine and threonine, they were represented by lysine and phenylalanine in sap samples from female trees. Further, Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis showed the presence of a proteinic band of 30 kDa only for the sap from male trees. Taken together, the sap from both male and female date palm trees had a number of properties that are highly valued by the functional food industry.

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Section: Electrophoretic Profile Of Date Palm Sapsupporting

confidence: 78%

Section: Sugar Profilementioning

confidence: 99%

Section: Electrophoretic Profile Of Date Palm Sapmentioning

confidence: 99%

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Physico-chemical properties and amino acid profiles of sap from Tunisian date palm

Makhlouf-Gafsi

Mokni-Ghribi

Bchir

et al. 2016

Sci. agric. (Piracicaba, Braz.)

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“…Date fruits consist of 70% carbohydrates; mostly as sugars, and 15-30% water. Dates are also a source of different minerals including iron, potassium and calcium, with low levels of sodium and fat (Al-Rawi et al, 1967;Thabet et al, 2010;Dayani et al, 2012).…”

Section: Nutritional Value Of Datesmentioning

confidence: 99%

Microbial contaminants of date palm (Phoenix dactylifera L.) in Iraqi tissue<br>culture laboratories

Abass

2013

Emir. J. Food Agric

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The date palm is one of the most important economic species of the palm family, grown mainly for its fruits (dates). Nowadays there is increased demand for date palm fruits around the world. To meet this demand, several propagation methods have been utilized, among them micropropagation which has been used in Iraq and many other countries for large-scale multiplication of date palm. Micropropagation faces several constraints; one is microbial contamination which represents a major challenge to the initiation and maintenance of date palm micropropagation laboratories. In recent years, two major groups of contaminants have been identified and isolated from different date palm tissue culture laboratories in Iraq. The first group is fungi. Several fungal species have been isolated and identified as contaminants; most predominant are: Aspergillus niger, Alternaria alternata and Penicillium spp. The second group is bacteria; predominantly of the genera Bacillus, Staphylococcus and Proteus.

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“…Rubber composites are mainly used for making ropes, hoses, belts, and mats [8]. NR has already been studied as a matrix for bionanocomposites with chitin whiskers [9], cellulose whiskers extracted from Syngonanthus nitens (Capim Dourado) [10], and rachis of palm tree [11]. It is possible to obtain fillers from different renewable resources depending on their biological origin, e.g., cellulose and starch [12], but main research is dedicated to cellulose whiskers, as this polymer is the most abundant on earth.…”

Section: Introductionmentioning

confidence: 99%

The use of rye, oat and triticale straw as fillers of natural rubber composites

et al. 2018

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Up to date, there are no evident research works carried out to improve and verify the physical, mechanical, barrier and damping properties of bioelastomer composites with addition of straw. Moreover, the problem of improving the reuse of straw is now demanding attention in the major cereal-growing areas of the world. The aim of this study was to receive knowledge of characteristic and producing multifunctional elastomer biocomposite filled with straw. Rye, oat and triticale were found to be active fillers improving wide range of rubber composites functional properties like: static mechanical, damping, barrier properties and hardness. The use of straw as a filler also increased cross-linking density. Dynamic-mechanical analysis indicates the presence of strongly developed secondary structure of examined fillers in vulcanizates. Moreover, all of the vulcanizates proved to be resistant to thermo-oxidative degradation. The application of straw as a filler allowed obtaining composites with lower value of oxygen index, that reflecting higher tendency of smoke generation. Introducing the straw into polymer matrix reduced the manufacturing costs of composites through the use of a significant amount of inexpensive and renewable filler. Furthermore straw fillers modify natural rubber, obtaining high-performance materials with meaningly enhanced applications.

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Characterisation of proteins from date palm sap (Phoenix dactylifera L.) by a proteomic approach

Cited by 18 publications

References 30 publications

Physico-chemical properties and amino acid profiles of sap from Tunisian date palm

Physico-chemical properties and amino acid profiles of sap from Tunisian date palm

Microbial contaminants of date palm (Phoenix dactylifera L.) in Iraqi tissue<br>culture laboratories

The use of rye, oat and triticale straw as fillers of natural rubber composites

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