Characterisation of potato isolates of <i>Dickeya chrysanthemi</i> in Spain by a microtitre system for biovar determination

Palacio-Bielsa, Ana; Cambra, M. A.; López, Marı́a M.

doi:10.1111/j.1744-7348.2006.00045.x

Cited by 44 publications

(50 citation statements)

References 26 publications

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“…These symptoms are in accordance with those observed in the field in Israel with natural infections, originating from seed tubers, confirming the first record of Dickeya sp. in Israel (Lumb et al 1986), and with symptoms described for disease expression in the summer in Spain (Palacio-Bielsa et al 2006). The pathogenicity results were in agreement with previous reports, demonstrating extensive stem-rot symptom expression associated with high relative humidity (RH) (100%) under the assay conditions (Palacio-Bielsa et al 2006), whereas lower RH (80%) induced more leaf desiccation and less rotting (Lumb et al 1986).…”

Section: Discussionsupporting

confidence: 81%

“…In Spain, Dickeya sp. expression was also associated with seed potato cvs Edzina, Kondor and Agria that had been produced in the Netherlands (Palacio-Bielsa et al 2006). Moreover, the previous outbreak of Dickeya sp.…”

Section: Discussionmentioning

confidence: 99%

“…is recognised as an important pathogen of potato (Pérombelon 2002) in many areas, including Northern Europe. It was recently reported in Spain (Palacio-Bielsa et al 2006) and in Finland (Joutsjoki et al 2005). The important Dickeya spp.…”

Section: Introductionmentioning

confidence: 89%

“…The strains were also classified using the microtiter plate assay described by Palacio-Bielsa et al (2006) with few modifications as described below. This included growth at 39°C, 41°C and 25°C (control) on nutrient broth (NB, OXOID; Dye 1969), anaerobic hydrolysis of arginine (Moeller 1955), and polysaccharide inulin utilisation in phenol red peptone water (inulin extracts were used from chicory and dahlia; in a 0.3% final concentration).…”

Section: Biochemical Identification and Classification Of Strainsmentioning

confidence: 99%

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Assessment of recent outbreaks of Dickeya sp. (syn. Erwinia chrysanthemi) slow wilt in potato crops in Israel

et al. 2008

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Suspected Dickeya sp. strains were obtained from potato plants and tubers collected from commercial plots. The disease was observed on crops of various cultivars grown from seed tubers imported from the Netherlands during the spring seasons of 2004-2006, with disease incidence of 2-30% (10% in average). In addition to typical wilting symptoms on the foliage, in cases of severe infection, progeny tubers were rotten in the soil. Six strains were characterised by biochemical, serological and PCR-amplification. All tests verified the strains as Dickeya sp. The rep-PCR and the biochemical assays showed that the strains isolated from blackleg diseased plants in Israel were very similar, if not identical to strains isolated from Dutch seed potatoes, suggesting that the infection in Israel originated from the Dutch seed. The strains were distantly related to D. dianthicola strains, typically found in potatoes in Western Europe, and were similar to biovar 3 D. dadanti or D. zeae. This is the first time that the presence of biovar 3 strains in potato in the Netherlands is described. One of the strains was used for pathogenicity assays on potato cvs Nicola and Mondial. Symptoms appeared 2 to 3 days after stem inoculation, and 7 to 10 days after soil inoculation. The control plants treated with water, or plants inoculated with Pectobacterium carotovorum, did not develop any symptoms with either method of inoculation. The identity of Dickeya sp. and P. carotovorum re-isolated from inoculated plants was confirmed by PCR and ELISA.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 89%

Section: Biochemical Identification and Classification Of Strainsmentioning

confidence: 99%

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Assessment of recent outbreaks of Dickeya sp. (syn. Erwinia chrysanthemi) slow wilt in potato crops in Israel

et al. 2008

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“…In contrast, strains from this group were less virulent in a field experiment in Finland with vacuuminfiltrated tubers. Also in Spain strains divergent from D. dianthicola, belonging to biovars 3 and 6, were isolated from potato (Palacio-Bielsa et al 2006). Tsror et al (2008) described the isolation of biovar 3 strains from blackleg-diseased potato plants in Israel, grown from Dutch seed.…”

Section: Introductionmentioning

confidence: 99%

Biochemical and genetical analysis reveal a new clade of biovar 3 Dickeya spp. strains isolated from potato in Europe

Slawiak

Beckhoven²,

Speksnijder³

et al. 2009

Eur J Plant Pathol

184

185

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Sixty-five potato strains of the soft rotcausing plant pathogenic bacterium Dickeya spp., and two strains from hyacinth, were characterised using biochemical assays, REP-PCR genomic finger printing, 16S rDNA and dnaX sequence analysis. These methods were compared with nineteen strains representing six Dickeya species which included the type strains. A group of twenty-two potato strains isolated between 2005-2007 in the Netherlands, Poland, Finland and Israel were characterised as belonging to biovar 3. They were 100% identical in REP-PCR, dnaX and 16S rDNA sequence analysis. In a polyphasic analysis they formed a new clade different from the six Dickeya species previously described, and may therefore constitute a new species. The strains were very similar to a Dutch strain from hyacinth. On the basis of dnaX sequences and biochemical assays, all other potato strains isolated in Europe between 1979 and 1994 were identified as D. dianthicola (biovar 1 and 7), with the exception of two German strains classified as D. dieffenbachia (biovar 2) and D. dadantii (biovar 3), respectively. Potato strains from Peru were classified as D. dadantii, from Australia as D. zeae and from Taiwan as D. chrysanthemi bv. parthenii, indicating that different Dickeya species are found in association with potato.

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Molecular Interactions of Pectobacterium and Dickeya with Plants

Gijsegem

Hugouvieux‐Cotte‐Pattat

Kraepiel

et al. 2021

Plant Diseases Caused by Dickeya and Pectobacterium Species

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Characterisation of potato isolates of Dickeya chrysanthemi in Spain by a microtitre system for biovar determination

Cited by 44 publications

References 26 publications

Assessment of recent outbreaks of Dickeya sp. (syn. Erwinia chrysanthemi) slow wilt in potato crops in Israel

Assessment of recent outbreaks of Dickeya sp. (syn. Erwinia chrysanthemi) slow wilt in potato crops in Israel

Biochemical and genetical analysis reveal a new clade of biovar 3 Dickeya spp. strains isolated from potato in Europe

Molecular Interactions of Pectobacterium and Dickeya with Plants

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