Characterisation of polymorphic microsatellite loci in three copper butterfly species (Lycaena spp.)

Trense, Daronja; Reim, Elisabeth; Fischer, Klaus

doi:10.1007/s11033-019-05056-3

Cited by 4 publications

(2 citation statements)

References 17 publications

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“…DNA was extracted with the E.Z.N.A.® Tissue DNA Kit (Omega Bio‐Tek) following the manufacturer's instructions. In this study, we used 14 and nine microsatellite markers for L. hippothoe and L. virgaureae (for details, see Trense et al, 2019; Table S2). Genotyping the microsatellites was performed on an ABI 3130XL sequencer (Applied Biosystems) and the length of microsatellite loci was determined using the program geneious 11.1.5 (Kearse et al, 2012; https://www.geneious.com).…”

Section: Methodsmentioning

confidence: 99%

The central Alps comprise a major dispersal barrier between western and eastern populations of two butterfly species

Trense

Jager

Fischer

2022

Journal of Biogeography

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Aim: Genetic connectivity and diversity are key components of species' resilience to environmental change. However, the specific factors shaping species' resilience are poorly understood. We here aim to strengthen our understanding of resilience in two butterfly species, by identifying regions of high genetic diversity and barriers to dispersal.Location: European Alps.Taxon: Copper butterflies (Lycaena spp.). Methods:We sampled 21 Lycaena hippothoe and 14 L. virgaureae populations with 18 individuals each in the European Alps. Using 14 and 9 microsatellite markers, respectively, we analysed genetic diversity and population structure (using arlequin, genalex, structure and snmf). We analysed spatial patterns of gene flow and genetic diversity (using an estimated effective migration surface), population demographic history and effective population size (diyabc), habitat suitability (maxent), and isolation by distance, and with resistance (genalex, circuitscape). Results:We found higher genetic diversity and a more pronounced genetic structure in L. hippothoe than in L. virgaureae. Both species displayed a major genetic barrier in the central Alps. Western and eastern populations showed the highest genetic diversity in L. hippothoe, but central ones in L. virgaureae. Populations of both species were structured by geodetic distance, and minimum and maximum altitude. Main Conclusions:The population genetic structure of both Copper butterflies seemed to be strongly affected by population history, demography and geographic features. Valleys and high mountain ridges seem to comprise major dispersal barriers.The high genetic diversity and pronounced population structure found in L. hippothoe seems to be related to a low dispersal ability and closed populations with high local abundances as opposed to L. virgaureae. We suggest that different conservation strategies are needed for these closely related species, focussing on connecting suitable habitats in L. hippothoe and increasing local population sizes in L. virgaureae.

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Section: Methodsmentioning

confidence: 99%

The central Alps comprise a major dispersal barrier between western and eastern populations of two butterfly species

Trense

Jager

Fischer

2022

Journal of Biogeography

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“…DNA was extracted with the E.Z.N.A.® Tissue DNA Kit (Omega bio‐tek, Düsseldorf, Germany) following the manufacturer's instructions. We used five microsatellite markers for L. helle and 14 for L. hippothoe , following the polymerase chain reaction (PCR) amplifications and thermal cycling profiles given in Habel et al (2008) and Trense et al (2019) (Table S1). Genotyping of microsatellites was performed on an ABI 3130XL sequencer (Applied Biosystems, Waltham, USA).…”

Section: Methodsmentioning

confidence: 99%

Contrasting genetic responses to habitat fragmentation for two Lycaenid butterfly species

Trense

Habel

Finger

et al. 2021

Insect Conserv Diversity

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Biodiversity is currently declining at the global scale. Apart from species declines and lowered abundances, the loss of genetic diversity is equally concerning as it may undermine fitness and the potential to adapt to future environmental change. We compared genetic diversity of historical and recent Alpine populations of two butterfly species, Lycaena helle and L. hippothoe, over a period of about 10 years. Using microsatellite markers, we found no changes over time in L. helle, while genetic diversity decreased, and differentiation increased in L. hippothoe. Lycaena helle inhabits peat bogs and wetland fallows with populations being strongly isolated, while L. hippothoe used to occur in population networks on hay meadows, with the latter being strongly exposed to agricultural intensification. We conclude that currently L. hippothoe populations are strongly declining due to changes in land use, resulting in genetic erosion potentially due to the collapse of population networks.

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Does specialisation affect genetic diversity in (pre-)Alpine populations of four species of Copper butterflies?

et al. 2021

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Characterisation of polymorphic microsatellite loci in three copper butterfly species (Lycaena spp.)

Cited by 4 publications

References 17 publications

The central Alps comprise a major dispersal barrier between western and eastern populations of two butterfly species

The central Alps comprise a major dispersal barrier between western and eastern populations of two butterfly species

Contrasting genetic responses to habitat fragmentation for two Lycaenid butterfly species

Does specialisation affect genetic diversity in (pre-)Alpine populations of four species of Copper butterflies?

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