Characterisation of Phenolic Compounds by HPLC–TOF/IT/MS in Buds and Open Flowers of ‘Chemlali’ Olive Cultivar

Abstract

The HPLC-TOF/IT/MS allowed the characterisation, for the first time, of the phenolic profile of extracts of 'Chemlali' olive buds and open flowers, proving to be a very useful technique for the characterisation and structure elucidation of phenolic compounds.

RP‐HPLC‐DAD‐ESI‐QTOF‐MS based metabolic profiling of the potential Olea europaea by‐product “wood” and its comparison with leaf counterpart

“…The second group contained: oleuropein aglycone ( 80 ), oleuropein isomers ( 70 and 71 ), demethyloleuropein ( 60 ), oleuropein hexosides ( 63 – 66 ), hydroxyoleuropein ( 61 and 62 ), methoxyoleuropein ( 69 ), and dihydro‐oleuropein ( 72 ). Their UV and MS/MS spectra matched well with those previously reported in other studies (Quirantes‐Piné et al, ; Taamalli et al, ; Talhaoui et al, ; Michel et al, ). Nevertheless, it is worth noticing that these compounds have not been reported in olive wood as was the case for olive leaves.…”

“…Thus, neutral losses of one (C 6 H 10 O 5 , 162.05 u) or two hexosyl groups (C 12 H 20 O 10 , 324.02 u) from the monoisotopic molecular ions, as well as deoxyhexosyl (i.e. rhamnoside group) and hexosyl moieties (C 12 H 20 O 9 , 308.11 u) were observed in the literature (Fu et al, ; Taamalli et al, ; Michel et al, ; Tóth et al, ; Ammar et al, , ). In contrast, apigenin 6,8‐di‐ C ‐glucoside ( 25 ) was characterised by the consecutive neutral losses of 18.01 (H 2 O), 60.02 (C 2 H 4 O 2 ), 90.03 (C 3 H 6 O 3 ), 120.04 (C 4 H 8 O 4 ), 180.06 (C 6 H 12 O 6 ) and/or 210.07 u (C 7 H 14 O 7, hexose plus CH 2 O) [Fig.…”

“…Thus, neutral losses of one (C 6 H 10 O 5 , 162.05 u) or two hexosyl groups (C 12 H 20 O 10 , 324.02 u) from the monoisotopic molecular ions, as well as deoxyhexosyl (i.e. rhamnoside group) and hexosyl moieties (C 12 H 20 O 9 , 308.11 u) were observed in the literature (Fu et al, 2010;Taamalli et al, 2013;Michel et al, 2015;Tóth et al, 2015;Ammar et al, 2015aAmmar et al, , 2015b Fig. 3(A)].…”

Putative identification of new p‐coumaroyl glycoside flavonoids in grape by ultra‐high performance liquid chromatography/high‐resolution mass spectrometry

“…Chrysoeriol‐7‐ O ‐glucoside is a polyphenol found in olive leaf extracts and in the buds and flowers of the 'Chemlali' olive cultivar . The p ‐coumaroylglucoside derivative was found in the stems and leaves of young Triticum durum (wheat) and has also been reported in some Labiatae species .…”

P-Glycoprotein, not BCRP, Limits the Brain Uptake of [18F]Mefway in Rodent Brain

“…Firstly, [ 18 F]Mefway showed less susceptibility to Bcrp activity. In humans, Bcrp was expressed at the BBB significantly more highly than P-gp based on mRNA and proteomic analysis [40,42]. Moreover, [ 18 F]Mefway has a higher target-to-reference value than [ 18 F]MPPF and considerably greater metabolic stability against radiodefluorination than [ 18 F]FCWAY in humans [14,17,43,44].…”