Characterisation of PEGylated PLGA nanoparticles comparing the nanoparticle bulk to the particle surface using UV/vis spectroscopy, SEC, 1H NMR spectroscopy, and X-ray photoelectron spectroscopy

Spek, S.; Haeuser, M.; Schaefer, Michael; Langer, Klaus

doi:10.1016/j.apsusc.2015.04.071

Cited by 38 publications

(35 citation statements)

References 29 publications

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“…There seems to be contradicting evidence in the literature, regarding the effect of PEGylation on PLGA NP size. Recently, Spek and co‐workers demonstrated that as the PEG content in PLGA NPs increases from non‐PEGylated to 15% PEG, a significant particle size increase is observed . The size increase was attributed to changes in viscosity of polymer solutions used in NP synthesis, as PEG content increases.…”

Section: Resultsmentioning

confidence: 99%

“…The size increase was attributed to changes in viscosity of polymer solutions used in NP synthesis, as PEG content increases. An increase in viscosity leads to reduced spontaneity of polymer self‐assemblage during the emulsification stage of NP synthesis resulting in the formation of larger particles . On the other hand, Beletsi, Rietscher and their respective co‐workers observed a decrease in particle size as PEG content increased .…”

Section: Resultsmentioning

confidence: 99%

“…Figure illustrates that for each of the PEGylated NPs, a characteristic singlet peak was observed at 3.65 ppm. Such a peak has been attributed to the protons in the ethylene units of the PEG . The peaks at 3.65 ppm were thereafter integrated to determine the area under curve (AUC).…”

Section: Resultsmentioning

confidence: 99%

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Influence of PEGylation on PLGA nanoparticle properties, hydrophobic drug release and interactions with human serum albumin

Samkange

D’Souza

Obikeze

et al. 2019

Journal of Pharmacy and Pharmacology

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Objective To evaluate the impact of PEG content on poly(lactic‐co‐glycolic acid) (PLGA) NP physicochemical properties, hydrophobic drug release (rifampicin as a model drug) and human serum protein binding. Methods Rifampicin loaded and unloaded nanoparticles with PEG content of 0–17% (w/w) were prepared by an emulsification–evaporation technique. Nanoparticles were characterized for size, zeta potential and morphology. PEGlyation was confirmed using proton nuclear magnetic resonance (1H NMR). Fluorescence spectroscopy and dynamic light scattering were used to determine nanoparticle‐protein binding, binding constants and stability of nanoparticles in human serum, respectively. Drug loading and release were determined by UV‐VIS spectroscopy and drug release data was mathematically modelled. Key findings A NP PEG content of 17% w/w significantly retarded release of rifampicin from PLGA NPs and altered kinetics of drug release. Stern–Volmer (Ksv) protein binding constants decreased upon PEG incorporation. A 2% w/w PEG was sufficient to significantly reduce protein binding extent to PLGA NPs and maintain particle size distributions. Conclusion The ability to fine tune drug release and formation of protein corona around nanoparticles is crucial to formulation scientists. This study suggests that PLGA NPs with low PEG content might be suitable for extended circulation and rapid drug release and that higher PEG content retards hydrophobic drug release.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Influence of PEGylation on PLGA nanoparticle properties, hydrophobic drug release and interactions with human serum albumin

Samkange

D’Souza

Obikeze

et al. 2019

Journal of Pharmacy and Pharmacology

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show abstract

“…Because a PDI value of less than 0.1 describes high monodispersity, samples with above a 5 wt % PEI content, which exhibits the highest zeta-potential values, are judged to be the most monodisperse colloidal suspensions. 16) In other words, the driving force of the dispersion behavior is electrostatic repulsion due to the positive surface charge. Figure 3 shows the particle size distribution parabola of the surface modified alumina particles with various concentrations of PEI.…”

Section: ¹1mentioning

confidence: 99%

Effective infiltration with polyethyleneimine-grafted colloidal alumina particles for silica-based ceramic cores

Choi

Kim

et al. 2017

J. Ceram. Soc. Japan

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Amine-functionalized alumina colloidal particles were prepared using polyethyleneimine (PEI) for enhancing the infiltration process of silica based ceramic core. The surface properties of PEI-grafted alumina particles were adjusted to improve their dispersion behavior in aqueous suspension, and the surface charge was adjusted to enhance their adsorption behavior, in neutral pH atmosphere, via an electrostatic repulsion force with a fused silica matrix containing an implied strong negative charge. The characteristics of the surface-modified particles were optimized as shown by the CH stretching vibration and NH bending vibration from FT-IR spectra, and the surface charge alternated from ¹3 to 45 mV. As a result, the dispersion behavior in aqueous solvent was advanced as indicated by the decrease of the polydispersity index, decrease of the average size from 178 to 58 nm, and sharpness of the particle size distribution parabola. These improvements greatly contributed to the acceleration of the infiltration time and efficiency (up to 30 min). Consequently, the promptly infiltrated specimens improved the flexural strength from 3.2 to 10.2 MPa and reduced the linear shrinkage rate from 1.91 to 1.05% as well.

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“…Surfactants can be used to minimize this effort [9]. In this work, SnO2 quantum dots are synthesized using a simple, low cost, less toxic sol gel method.…”

Section: Introductionmentioning

confidence: 99%

Preparation of tin oxide quantum dots

Fauzi

Kamarulzaman

Kasim

et al. 2017

AIP Conference Proceedings

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Characterisation of PEGylated PLGA nanoparticles comparing the nanoparticle bulk to the particle surface using UV/vis spectroscopy, SEC, 1H NMR spectroscopy, and X-ray photoelectron spectroscopy

Cited by 38 publications

References 29 publications

Influence of PEGylation on PLGA nanoparticle properties, hydrophobic drug release and interactions with human serum albumin

Influence of PEGylation on PLGA nanoparticle properties, hydrophobic drug release and interactions with human serum albumin

Effective infiltration with polyethyleneimine-grafted colloidal alumina particles for silica-based ceramic cores

Preparation of tin oxide quantum dots

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