Characterisation of palytoxin from an undescribed Palythoa (Anthozoa: Zoantharia: Sphenopidae) with significant in vitro cytotoxic effects on cancer cells at picomolar doses

Sawelew, Ludovic; Gault, F; Nuccio, Christopher; Perez, Yvan; Lorquin, Jean

doi:10.1101/292219

Cited by 3 publications

(1 citation statement)

References 75 publications

(116 reference statements)

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“…aff . clavata is 90 times more toxic than P. caribaeorum (Béress et al, 1983; Sawelew et al, 2018), which could explain the more active predation upon P. caribaeorum regardless of the experimental environmental conditions. Moreover, it was shown that the simulated low‐pH conditions decreased the rate of consumption of P .…”

Section: Discussionmentioning

confidence: 99%

Effects of low pH and high temperature on two Palythoa spp. and predator–prey interactions in the subtropical eastern Atlantic

López

Bas-Silvestre

Rodríguez

et al. 2020

Aquatic Conservation

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In the current context of climate change, benthic cnidarians of the genus Palythoa have been suggested to be resistant owing to their intrinsic biological characteristics. In tropical regions, some species are currently proliferating in areas where environmental conditions are less suitable for other organisms, even replacing hard coral ecosystems. Considering their tropical affinities, phase‐shifts towards Palythoa‐dominated areas could become more frequent in future climate change scenarios, leading to changes in ecosystem organization. The aim of this study was to evaluate the effect of climate change stressors in two common Palythoa spp. with different habitat affinities within a subtropical region, and the effect upon their predator–prey interactions. The results of this experimental study demonstrated that colonies of P. aff. clavata and P. caribaeorum were significantly affected by exposure to temperature and pH conditions predicted for 2100 in the Canary Islands, during 62 days. Despite zoantharians’ lack of carbonate in their body wall, Palythoa spp. were most affected in their growth rates by lowered pH, and colonies significantly decreased in weight and size. Although all colonies exhibited symptoms of bleaching at high temperature, a reduction in chlorophyll content was also observed at low pH. Predation by Platypodiella picta crabs decreased on P. aff. clavata exposed to acidic conditions, which may compensate for the lowered ecological performance of the species in these climate change conditions. In contrast, P. picta was able to actively feed on P. caribaeorum colonies regardless of the experimental conditions. Despite being suggested as winner species in a climate change scenario, our study demonstrated that low pH negatively impacted Palythoa spp. survival. If the species are not able to acclimatize to the new conditions, changes in their populations may be expected, although their magnitude could be ameliorated by means of a decrease in predation rates.

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Section: Discussionmentioning

confidence: 99%

Effects of low pH and high temperature on two Palythoa spp. and predator–prey interactions in the subtropical eastern Atlantic

López

Bas-Silvestre

Rodríguez

et al. 2020

Aquatic Conservation

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Soritesidine, a Novel Proteinous Toxin from the Okinawan Marine Sponge Spongosorites sp.

et al. 2019

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A novel protein, soritesidine (SOR) with potent toxicity was isolated from the marine sponge Spongosorites sp. SOR exhibited wide range of toxicities over various organisms and cells including brine shrimp (Artemia salina) larvae, sea hare (Aplysia kurodai) eggs, mice, and cultured mammalian cells. Toxicities of SOR were extraordinary potent. It killed mice at 5 ng/mouse after intracerebroventricular (i.c.v.) injection, and brine shrimp and at 0.34 µg/mL. Cytotoxicity for cultured mammalian cancer cell lines against HeLa and L1210 cells were determined to be 0.062 and 12.11 ng/mL, respectively. The SOR-containing fraction cleaved plasmid DNA in a metal ion dependent manner showing genotoxicity of SOR. Purified SOR exhibited molecular weight of 108.7 kDa in MALDI-TOF MS data and isoelectric point of approximately 4.5. N-terminal amino acid sequence up to the 25th residue was determined by Edman degradation. Internal amino acid sequences for fifteen peptides isolated from the enzyme digest of SOR were also determined. None of those amino acid sequences showed similarity to existing proteins, suggesting that SOR is a new proteinous toxin.

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Emerging Optical Materials in Sensing and Discovery of Bioactive Compounds

Vaz

Valpradinhos

Frasco

et al. 2021

Sensors

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Optical biosensors are used in numerous applications and analytical fields. Advances in these sensor platforms offer high sensitivity, selectivity, miniaturization, and real-time analysis, among many other advantages. Research into bioactive natural products serves both to protect against potentially dangerous toxic compounds and to promote pharmacological innovation in drug discovery, as these compounds have unique chemical compositions that may be characterized by greater safety and efficacy. However, conventional methods for detecting these biomolecules have drawbacks, as they are time-consuming and expensive. As an alternative, optical biosensors offer a faster, simpler, and less expensive means of detecting various biomolecules of clinical interest. In this review, an overview of recent developments in optical biosensors for the detection and monitoring of aquatic biotoxins to prevent public health risks is first provided. In addition, the advantages and applicability of these biosensors in the field of drug discovery, including high-throughput screening, are discussed. The contribution of the investigated technological advances in the timely and sensitive detection of biotoxins while deciphering the pathways to discover bioactive compounds with great health-promoting prospects is envisaged to meet the increasing demands of healthcare systems.

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Characterisation of palytoxin from an undescribed Palythoa (Anthozoa: Zoantharia: Sphenopidae) with significant in vitro cytotoxic effects on cancer cells at picomolar doses

Cited by 3 publications

References 75 publications

Effects of low pH and high temperature on two Palythoa spp. and predator–prey interactions in the subtropical eastern Atlantic

Effects of low pH and high temperature on two Palythoa spp. and predator–prey interactions in the subtropical eastern Atlantic

Soritesidine, a Novel Proteinous Toxin from the Okinawan Marine Sponge Spongosorites sp.

Emerging Optical Materials in Sensing and Discovery of Bioactive Compounds

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