Characterisation of One Class of Group III Sensory Neurons Innervating Abdominal Muscles of the Mouse

Peterson, R.A.; Barry, Christine; Wiklendt, Lukasz; Brookes, Simon Jonathan

doi:10.1016/j.neuroscience.2019.09.019

Cited by 2 publications

(13 citation statements)

References 61 publications

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“…HCAR1-like immunoreactivity was also visible in some nerve fibers in whole-mount preparations of abdominal muscles, including in CGRP-negative axons. The presence of HCAR1+/CGRP-immunoreactive cell bodies and muscle axons is consistent with the expression of HCAR1 by CT3 afferents because CT3 afferents do not express CGRP (Peterson et al, 2019). However, a recent study (de Castro Abrantes et al, FIGURE 7 | mRNA expression of HCAR1 across tissue samples (n = 4).…”

Section: Discussionsupporting

confidence: 74%

“…First, CT3 muscle afferents were identified by their characteristic, saturating responses to graded von Frey hair probing and to stretch (Peterson et al, 2019 ). A 1 mN von Frey hair was applied (units 14, n = 13), to map the mechanosensitive receptive field of each unit.…”

Section: Resultsmentioning

confidence: 99%

“…These could be compared in the same software to clusters derived from amplitude/duration discrimination. Results are presented from 32 afferent recordings ( n = 22), in which 19 afferents were positively identified as CT3 afferents (Peterson et al, 2019 ), nine demonstrated muscle spindle characteristics, and four units could not be identified.…”

Section: Methodsmentioning

confidence: 99%

“…CT3 afferents were positively identified by using criteria, previously described in Peterson et al ( 2019 ), these included the typical characteristics of low threshold saturating responses to von Frey hairs and to stretch by applied load, under isotonic conditions.…”

Section: Methodsmentioning

confidence: 99%

“…These were referred to as “CT3” afferents. They had saturating responses to low levels of muscle stretch and could be potently activated by a metabolite mix comprising adenosine triphosphate (ATP), lactate, and low pH (Peterson et al, 2019 ). The signaling mechanisms of both ATP and low pH have been well characterized.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Lactate on One Class of Group III (CT3) Muscle Afferents

Peterson

König

Zimmermann

et al. 2020

Front. Cell. Neurosci.

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A class of Group III muscle afferent neurons has branching sensory terminals in the connective tissue between layers of mouse abdominal muscles ("CT3 muscle afferents"). These sensory endings are both mechanosensitive and metabosensitive. In the present study, responses of CT3 afferents to lactate ions and changes in temperature were recorded. Raising muscle temperature from 32.7 • C to 37 • C had no consistent effects on CT3 afferent basal firing rate or responses to either von Frey hair stimulation or to an applied load. Superfusion with lactate ions (15 mM, pH 7.4) was associated with an increase in firing from 6 ± 0.7 Hz to 11.7 ± 6.7 Hz (14 units, n = 13, P < 0.05, P = 0.0484) but with considerable variability in the nature and latency of response. Reducing the concentration of extracellular divalent cations, which mimicked the chelating effects of lactate, did not increase firing. Raised concentrations of divalent cations (to compensate for chelation) did not block excitatory effects of lactate on CT3 afferents, suggesting that effects via ASIC3 were not involved. Messenger RNA for the G-protein coupled receptor, hydroxyl carboxylic acid receptor 1 (HCAR1) was detected in dorsal root ganglia and HCAR1-like immunoreactivity was present in spinal afferent nerve cell bodies retrogradely labeled from mouse abdominal muscles. HCAR1-like immunoreactivity was also present in axons in mouse abdominal muscles. This raises the possibility that some effects of lactate on group III muscle afferents may be mediated by HCAR1.

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Section: Discussionsupporting

confidence: 74%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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