Characterisation of natural polysaccharides (plant gums) used as binding media for artistic and historic works by capillary zone electrophoresis

Groessl, Michael; Harrison, Sabine M.; Kaml, Isabella; Kenndler, Ernst

doi:10.1016/j.chroma.2005.04.075

Cited by 52 publications

(19 citation statements)

References 34 publications

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“…28) For achieving an accurate identification of complicated carbohydrate, the separation of a bigger number of monosaccharides was inevitably required. 13,22,24) From the study previously, the sucrose, glucose and fructose in C. pilosula plant have been identified by capillary electrophoretic method with amperometric detection. 10) However, in this study an efficient method for the determination of the aldose composition in the plant polysaccharide from C. pilosula was achieved for the first time and seven composition monosaccharides were successfully identified and the assay results were satisfactory.…”

Section: Discussionmentioning

confidence: 99%

“…Although the principle of the complexation between borate and vicinal hydroxy groups in sugars to form negative complexes was widely used for the separation of carbohydrate compounds, [19][20][21][22][23] to the best of our knowledge rather limited monosaccharides were usually baseline separated, which significantly affected the analytical accuracy. 13,24) Therefore, it is obvious that the improved separation of a series of monosaccharides needs to be further investigated in the presence of borate complexation.…”

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Development and Application of a Capillary Electrophoretic Method for the Composition Analysis of a Typical Heteropolysaccharide from Codonopsis pilosula NANNF.

Yang

Zhao

Ruan

et al. 2008

Biological & Pharmaceutical Bulletin

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A simple capillary electrophoretic method based on 1-phenyl-3-methyl-5-pyrazolon (PMP) derivatization has been developed for simultaneous separation and determination of nine aldoses (xylose, arabinose, glucose, rhamnose, fucose, galactose, mannose, glucuronic acid and galacturonic acid). The separation of PMP-labeled monosaccharide derivatives was carried out in uncoated capillary (48.5 cm؋75 m mm i.d.) and under the selected optimum conditions of pH 11.0, 200 mM borate buffer at applied voltage 15 kV and capillary temperature 20°C, the nine PMP-monosaccharides could be perfectly separated from each other within 40 min. Furthermore, the developed method was firstly applied to determine the sugar composition in the polysaccharide isolated from Chinese Codonopsis pilosula. The results showed that C. pilosula polysaccharide was a typically acidic heteropolysaccharide and was composed of arabinose, glucose, rhamnose, galactose, mannose, glucuronic acid and galacturonic acid in the molar contents of 48.1, 103.5, 16.1, 48.5, 7.5, 4.2 and 119.1 m mM, respectively. The assay results were satisfactory. Key words Codonopsis pilosula; heteropolysaccharide; composition analysis; capillary zone electrophoresisBiol. Pharm. Bull. 31(10) 1860-1865 (2008) © 2008 Pharmaceutical Society of Japan * To whom correspondence should be addressed. e-mail: xbyang@snnu.edu.cn MATERIALS AND METHODS Materials and ReagentsThe plant materials of the roots of C. pilosula were harvested from the region of Qinling Mountains, Shaanxi province, China and were identified according to the identification standard of Pharmacopeia of the People's Republic of China. The plant materials were thoroughly air-dried and finely powdered.1-Phenyl-3-methyl-5-pyrazolone (PMP), purchased from Beijing Reagent Plant (Beijing, China), was re-crystallized twice from chromatographic grade methanol before use. DMannose, L-rhamnose, D-glucuronic acid, D-galacturonic acid, D-glucose, D-xylose, D-galactose, L-arabinose, D-fucose were all obtained from Sigma (St. Louis, U.S.A.). Trifluoroacetic acid (TFA) was obtained from Merck (Darmstadt, Germany). HPLC grade acetonitrile and methanol were purchased from TEDIA (U.S.A.). Boric acid and sodium hydroxide were obtained from Sigma (U.S.A.). HPLC grade methanol (MeOH) was from TEDIA (U.S.A.). All aqueous solutions were made up in the Milli-Q system water unless mentioned otherwise. All the other chemicals were of analytical grade.Isolation of C. Pilosula Polysaccharide The C. pilosula polysaccharide was isolated by hot-water extraction and ethanol precipitation. The dried materials of Chinese C. pilosula (550 g) were defatted with 95% alcohol and then extracted with distilled water (g/mlϭ1 : 10) for 3 h. After each three-hour period of water extracting, the water extracts were collected and the residues were extracted again for 3 cycles. The combined extracts were pooled, concentrated to 30% of the original volume under a reduced pressure and then centrifuged at 3000 rpm for 15 min. The supernatant was collected and 3 volum...

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Development and Application of a Capillary Electrophoretic Method for the Composition Analysis of a Typical Heteropolysaccharide from Codonopsis pilosula NANNF.

Yang

Zhao

Ruan

et al. 2008

Biological & Pharmaceutical Bulletin

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“…Resolution can be improved through alteration of analyte characteristics, including the introduction of charge, which is very helpful for the analysis of neutral oligosaccharides (uncharged at pH ,12) by CE. Such precolumn derivatization has been used to quantify oligosaccharides in enzyme-treated digests from wood and pulp [61], plant gums [62], antibody pharmaceuticals [39,63], and other sources [34-37, 47, 48, 64], and naturally occurring oligosaccharides in biological samples including blood [65] and human milk [66].…”

Section: Derivatized Oligosaccharides With Uv or Lifmentioning

confidence: 99%

Capillary electrophoresis of acidic oligosaccharides from human milk

Bao

Newburg

2008

Electrophoresis

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Interest in defining the array of oligosaccharides of human milk has been increasing. Pathogens that bind glycans on their host mucosal surfaces may be inhibited by human milk oligosaccharides. It has been postulated that acidic oligosaccharides in human milk may inhibit binding by pathogens that bind acidic glycans in the gut, but testing this hypothesis requires their reliable quantification in milk. Sialyloligosaccharides of human milk have been quantified by HPLC and CE. A recent CE technique uses the MEKC mode with direct detection at 205 nm to resolve and quantify, in the native form, the 12 most dominant sialyloligosaccharides of human milk in a single 35-min run. The method gives a linear response from 39 to 2500 microg/mL with a coefficient of variation between 2 to 9% and accuracy from 93 to 109%. This was used to detect variation in expression of specific sialyloligosaccharides in milk. Individual sialyloligosaccharide concentrations in milk differ among individual donors and between less and more mature milk. Thus, CE can be used to measure variation in sialyloligosaccharide expression in milk, and thereby test the relationship of this variation-to-variation in risk of specific diseases in breastfed infants.

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“…We have successfully introduced capillary electrophoresis (CE) as an analytical method to characterise natural organic binders, including plant gums [25,26], animal glues [26][27][28] and drying oils [29,30] in objects of historic or artistic interest (see e.g. our recent review [31]).…”

Section: Introductionmentioning

confidence: 99%

Capillary Electrophoresis as Analytical Method for the Characterisation of Natural Diterpenoid Resins in Artistic and Historic Works: A Comparison with Gas Chromatography

Dell‘mour¹,

Findeisen²,

Kaml³

et al. 2008

TOACJ

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Abstract:We have applied capillary electrophoresis to separate and detect the characteristic main components of diterpenoic resins applied as binders in museum objects, i.e. the abietane and pimarane acids and larixol acetate. Under the influence of the applied high voltage of 15000 V these analytes move in a narrow separation capillary with 50 m inner diameter. This open tube has 48.5 cm total length, with 40.0 cm distance to the detector. Electrophoretic migration is supported (and, in case of the electrically neutral larixol acetate, enabled) by charged additives to the running buffer, which form ionic complexes with the analytes. The separated analytes are detected within less than 15 min by their UV absorbance measured at characteristic wavelengths of 200, 250 and 270 nm. In contrast to chromatographic methods, no derivatisation is needed. As in gas chromatography, identification of the resins is carried out by the peak patterns based on the resin composition. From the comparison with established GC methodology for the analysis of resins it can be concluded that the analytical performance parameters of the capillary electrophoretic method in terms of resolution and analysis time are comparable to those from gas chromatography, whereas the detection limit is higher in general. However, sample pretreatment is simpler for capillary electrophoresis; it consists only in the dissolution of the sample in methanol.

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Characterisation of natural polysaccharides (plant gums) used as binding media for artistic and historic works by capillary zone electrophoresis

Cited by 52 publications

References 34 publications

Development and Application of a Capillary Electrophoretic Method for the Composition Analysis of a Typical Heteropolysaccharide from Codonopsis pilosula NANNF.

Development and Application of a Capillary Electrophoretic Method for the Composition Analysis of a Typical Heteropolysaccharide from Codonopsis pilosula NANNF.

Capillary electrophoresis of acidic oligosaccharides from human milk

Capillary Electrophoresis as Analytical Method for the Characterisation of Natural Diterpenoid Resins in Artistic and Historic Works: A Comparison with Gas Chromatography

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