Characterisation of methionine adenosyltransferase from Mycobacterium smegmatis and M. tuberculosis

Berger, Bradley J.; Knodel, Marvin H.

doi:10.1186/1471-2180-3-12

Cited by 27 publications

(10 citation statements)

References 53 publications

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“…S9 and Text S1 in the supplemental material). Therefore, if Aza affects the synthesis of methionine adenosyltransferase (57) and consequently the production of SAM, the activity of EgtD may have been modulated, thus affecting the production of ERG (Table 3). However, it remains unclear why it would result in an increase (Table 3) in the production of ERG instead of a decrease.…”

Section: Resultsmentioning

confidence: 99%

Compounds with Potential Activity against Mycobacterium tuberculosis

Emani

Williams

Wiid

et al. 2018

Antimicrob Agents Chemother

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The high acquisition rate of drug resistance by necessitates the ongoing search for new drugs to be incorporated in the tuberculosis (TB) regimen. Compounds used for the treatment of other diseases have the potential to be repurposed for the treatment of TB. In this study, a high-throughput screening of compounds against thiol-deficient strains and subsequent validation with thiol-deficient strains revealed that and mutants had increased susceptibility to azaguanine (Aza) and sulfaguanidine (Su); and mutants had increased susceptibility to bacitracin (Ba); and, , and mutants had increased susceptibility to fusaric acid (Fu). Further analyses revealed that some of these compounds were able to modulate the levels of thiols and oxidative stress in This study reports the activities of Aza, Su, Fu, and Ba against and provides a rationale for further investigations.

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Section: Resultsmentioning

confidence: 99%

Compounds with Potential Activity against Mycobacterium tuberculosis

Emani

Williams

Wiid

et al. 2018

Antimicrob Agents Chemother

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“…7] [246]. In the first step, S -denosyl methionine transferase or S -adenosyl methionine synthase which is denoted by metK , catalyzes the conversion of methionine to S -adenosine-L-methionine [340]. S -adenosyl-L-methionine dependent methyl transferase (Rv2118c) catalyzes the conversion of S -adenosyl-L-methionine to S -adenosine homocysteine [341].…”

Section: Methionine Biosynthesis and Reverse Transsulfurationmentioning

confidence: 99%

New Targets and Inhibitors of Mycobacterial Sulfur Metabolism

Paritala¹,

Carroll²

2013

IDDT

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The identification of new antibacterial targets is urgently needed to address multidrug resistant and latent tuberculosis infection. Sulfur metabolic pathways are essential for survival and the expression of virulence in many pathogenic bacteria, including Mycobacterium tuberculosis. In addition, microbial sulfur metabolic pathways are largely absent in humans and therefore, represent unique targets for therapeutic intervention. In this review, we summarize our current understanding of the enzymes associated with the production of sulfated and reduced sulfur-containing metabolites in Mycobacteria. Small molecule inhibitors of these catalysts represent valuable chemical tools that can be used to investigate the role of sulfur metabolism throughout the Mycobacterial lifecycle and may also represent new leads for drug development. In this light, we also summarize recent progress made in the development of inhibitors of sulfur metabolism enzymes.

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“…Mycobacterium tuberculosis MAT [7,15,35,39,40]. The AdoMet synthesizing activity of some of these dimeric MATs is stimulated by DMSO at low methionine concentrations [7].…”

Section: Homodimeric Mats That Includes Mammalian Mat III Archaeal Mmentioning

confidence: 99%

“…Most members of the MAT family are homo-oligomers except for mammalian MAT II, and the majority are tetramers with the exception of a few dimers (i.e. mammalian MAT III and archaeal 4 MATs) [7,15]. All the crystal structures known to date (mammalian and E. coli MATs)…”

Section: Introductionmentioning

confidence: 99%