Characterisation of metal–chelate methacrylate monoliths

Peterka, Matjaž; Jarc, Marko; Banjac, Marko; Frankovič, Vida; Benčina, Katja; Merhar, M.; Gaberc-Porekar, Vladka; Menart, Viktor; Štrancar, Aleš; Podgornik, Aleš

doi:10.1016/j.chroma.2005.08.057

Cited by 27 publications

(13 citation statements)

References 18 publications

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“…The use of monolithic columns in the effective purification of many recombinant proteins and proteins from its natural sources had been reported [13]. Peterka et al reported the isolation of histidine containing proteins to high purity using CIM-IMAC with a single chromatographic step [14]. The evenly distributed ligands on CIM discs compared to soft gels facilitate the bound proteins to be well distributed on the matrix, preventing protein aggregation.…”

Section: Resultsmentioning

confidence: 99%

Recovery of active anti TNF-α ScFv through matrix-assisted refolding of bacterial inclusion bodies using CIM monolithic support

Krishnan

Bilgimol

Vijayalakshmi

et al. 2012

Journal of Chromatography B

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Section: Resultsmentioning

confidence: 99%

Recovery of active anti TNF-α ScFv through matrix-assisted refolding of bacterial inclusion bodies using CIM monolithic support

Krishnan

Bilgimol

Vijayalakshmi

et al. 2012

Journal of Chromatography B

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“…Peterka et al [125] demonstrated IMAC on a CIM disk. They demonstrated very selective affinity for histidinecontaining proteins with a high binding capacity of up to 20 mg/mL of monolith.…”

Section: Cim Disk For Affinity Extractionsmentioning

confidence: 98%

“…They modified the epoxy groups on the CIM by a method adapted from work by Luo et al [126]. Elution was achieved by displacement using imidazole and the authors were able to selectively extract protein LK-801 and green fluorescence protein with six histidine tags (green fluorescent protein (GFP)-6His) [125] as shown in Fig. 9.…”

Section: Cim Disk For Affinity Extractionsmentioning

confidence: 98%

Porous polymer monoliths for extraction: Diverse applications and platforms

Potter

Hilder²

2008

J of Separation Science

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Polymer monoliths are becoming increasingly popular as sorbent materials, and, along with silica monoliths, they are sometimes touted as replacements for the particulate stationary phases used in HPLC. This critical and prospective review shows how polymer monoliths are in fact finding numerous extraction roles that do not resemble HPLC. They are showing great promise as extractors in a remarkable range of platforms, formats and hyphenated systems with functions ranging from chromatographic preconcentration to large-scale preparative extraction. Monolith surface chemistry, morphology and the approaches to monolith synthesis are discussed with regards to these emerging roles.

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“…Several types of monoliths have been employed in IMAC, ranging from silica monoliths to polymethacrylate-based supports and cryogels [49, 50, 60, 91, 92]. One previous application utilized a silica monolith capillary column that was functionalized with IDA and Fe 3+ .…”

Section: Monoliths In Immobilized Metal-ion Affinity Chromatography (mentioning

confidence: 99%

Affinity monolith chromatography: a review of principles and recent analytical applications

et al. 2012

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Affinity monolith chromatography (AMC) is a type of liquid chromatography that uses a monolithic support and a biologically-related binding agent as a stationary phase. AMC is a powerful method for the selective separation, analysis or studies of specific target compounds in a sample. This review discusses the basic principles of AMC and recent developments or applications of this method, with particular emphasis being given to work that has appeared in the last five years. Various materials that have been used to prepare columns for AMC are examined, including organic monoliths, silica monoliths, agarose monoliths and cryogels. These supports have been used in AMC for formats that have ranged from traditional columns to disks, microcolumns and capillaries. Many binding agents have also been employed in AMC, such as antibodies, enzymes, proteins, lectins, immobilized metal-ions and dyes. Some applications that have been reported with these binding agents in AMC are bioaffinity chromatography, immunoaffinity chromatography or immunoextraction, immobilized metal-ion affinity chromatography, dye-ligand affinity chromatography, chiral separations and biointeraction studies. Examples are presented from fields that include analytical chemistry, pharmaceutical analysis, clinical testing and biotechnology. Current trends and possible future directions in AMC are also discussed.

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Characterisation of metal–chelate methacrylate monoliths

Cited by 27 publications

References 18 publications

Recovery of active anti TNF-α ScFv through matrix-assisted refolding of bacterial inclusion bodies using CIM monolithic support

Recovery of active anti TNF-α ScFv through matrix-assisted refolding of bacterial inclusion bodies using CIM monolithic support

Porous polymer monoliths for extraction: Diverse applications and platforms

Affinity monolith chromatography: a review of principles and recent analytical applications

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