Characterisation of membrane phospholipids and glycolipids from a halophilic archaebacterium by high-performance liquid chromatography/electrospray mass spectrometry

Qiu, Difei; Games, Marguerite P. L.; Xiao, X.-Y.; Games, David E.; Walton, Terence J.

doi:10.1002/1097-0231(20000915)14:17<1596::aid-rcm66>3.0.co;2-5

Cited by 22 publications

(9 citation statements)

References 11 publications

(26 reference statements)

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“…While unsaturated lipids from archaea have been previously reported (20), concerns with analytical methodologies limited the veracity of the reports. As a result of improvements in analytical approaches, such as electrospray/high-performance liquid chromatography-mass spectrometry (ES/HPLC-MS) of intact lipids, the presence of unsaturated diether lipids (UDLs) has been confirmed in archaea (23,24).…”

supporting

confidence: 92%

Cold Adaptation in the Antarctic Archaeon Methanococcoides burtonii Involves Membrane Lipid Unsaturation

Nichols¹,

Miller²,

et al. 2004

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Direct analysis of membrane lipids by liquid chromatography-electrospray mass spectrometry was used to demonstrate the role of unsaturation in ether lipids in the adaptation of Methanococcoides burtonii to low temperature. A proteomics approach using two-dimensional liquid chromatography-mass spectrometry was used to identify enzymes involved in lipid biosynthesis, and a pathway for lipid biosynthesis was reconstructed from the M. burtonii draft genome sequence. The major phospholipids were archaeol phosphatidylglycerol, archaeol phosphatidylinositol, hydroxyarchaeol phosphatidylglycerol, and hydroxyarchaeol phosphatidylinositol. All phospholipid classes contained a series of unsaturated analogues, with the degree of unsaturation dependent on phospholipid class. The proportion of unsaturated lipids from cells grown at 4°C was significantly higher than for cells grown at 23°C. 3-Hydroxy-3-methylglutaryl coenzyme A synthase, farnesyl diphosphate synthase, and geranylgeranyl diphosphate synthase were identified in the expressed proteome, and most genes involved in the mevalonate pathway and processes leading to the formation of phosphatidylinositol and phosphatidylglycerol were identified in the genome sequence. In addition, M. burtonii encodes CDP-inositol and CDP-glycerol transferases and a number of homologs of the plant geranylgeranyl reductase. It therefore appears that the unsaturation of lipids may be due to incomplete reduction of an archaeol precursor rather than to a desaturase mechanism. This study shows that cold adaptation in M. burtonii involves specific changes in membrane lipid unsaturation. It also demonstrates that global methods of analysis for lipids and proteomics linked to a draft genome sequence can be effectively combined to infer specific mechanisms of key biological processes.

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supporting

confidence: 92%

Cold Adaptation in the Antarctic Archaeon Methanococcoides burtonii Involves Membrane Lipid Unsaturation

Nichols¹,

Miller²,

et al. 2004

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“…In addition, different isolates designated as Halorubrum saccharovorum appeared to contain different glycolipids (Tindall, 1990b). A recent report that claims that a sulfated diglycosyl-2,3-di-O-phytanyl-sn-glycerol may be the major glycolipid of Halobacterium salinarum strain R 1 (rather that S-TGD-1 and S-TeGD; Qiu et al, 2000) also does not agree with other published data.…”

Section: The Cytoplasmic Membrane and Its Lipidsmentioning

confidence: 76%

“…Considerable effort has been devoted to the structural elucidation of the polar and neutral lipids found in the different representatives of the family. Analytical methods such as mass spectrometry (fast atom bombardment mass spectrometry, HPLC combined with electrospray mass spectrometry), and NMR spectroscopical techniques are useful in the structural characterization of lipid mixtures, isolated lipid components, or chemical degradation products of these lipids (Fredrickson et al, 1989a(Fredrickson et al, , 1989bKlöppel and Fredrickson, 1991;Qiu et al, 2000). One-or twodimensional thin-layer chromatography is a useful technique for the rapid characterization of the lipids present in halophilic archaeal isolates Torreblanca et al, 1986).…”

Section: The Cytoplasmic Membrane and Its Lipidsmentioning

confidence: 99%

Halophilic Microorganisms and their Environments

Oren

2002

Cellular Origin, Life in Extreme Habitats and Astrobiology

401

294

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“…The mass of the lipid from spot 5 was 1,055.9 Da. This corresponds to the mass of sulfodiglycosyldiglyceride (S-DGD), a negatively charged glycolipid typical of various Halorubrum strains and of certain other halophilic microorganisms (30,33,45). These data strongly suggest the presence of nonphosphorus S-DGD in HRPV-1 and its host.…”

Section: Resultsmentioning

confidence: 99%

The Single-Stranded DNA Genome of Novel Archaeal Virus Halorubrum Pleomorphic Virus 1 Is Enclosed in the Envelope Decorated with Glycoprotein Spikes

Pietilä

Laurinavičius

Sund

et al. 2010

J Virol

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Only a few archaeal viruses have been subjected to detailed structural analyses. Major obstacles have been the extreme conditions such as high salinity or temperature needed for the propagation of these viruses. In addition, unusual morphotypes of many archaeal viruses have made it difficult to obtain further information on virion architectures. We used controlled virion dissociation to reveal the structural organization of Halorubrum pleomorphic virus 1 (HRPV-1) infecting an extremely halophilic archaeal host. The singlestranded DNA genome is enclosed in a pleomorphic membrane vesicle without detected nucleoproteins. VP4, the larger major structural protein of HRPV-1, forms glycosylated spikes on the virion surface and VP3, the smaller major structural protein, resides on the inner surface of the membrane vesicle. Together, these proteins organize the structure of the membrane vesicle. Quantitative lipid comparison of HRPV-1 and its host Halorubrum sp. revealed that HRPV-1 acquires lipids nonselectively from the host cell membrane, which is typical of pleomorphic enveloped viruses.

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Characterisation of membrane phospholipids and glycolipids from a halophilic archaebacterium by high-performance liquid chromatography/electrospray mass spectrometry

Cited by 22 publications

References 11 publications

Cold Adaptation in the Antarctic Archaeon Methanococcoides burtonii Involves Membrane Lipid Unsaturation

Cold Adaptation in the Antarctic Archaeon Methanococcoides burtonii Involves Membrane Lipid Unsaturation

Halophilic Microorganisms and their Environments

The Single-Stranded DNA Genome of Novel Archaeal Virus Halorubrum Pleomorphic Virus 1 Is Enclosed in the Envelope Decorated with Glycoprotein Spikes

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