Characterisation of mammary tumour virus of strain ICRC mouse

Karande, K A; Joshi, Bhumika; Talageri, V. R.; Dumaswala, Ranjana; Ranadive, K J

doi:10.1016/0014-2964(78)90188-3

Cited by 3 publications

(2 citation statements)

References 23 publications

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“…The distant pattern of the polypeptide composition of our MT-A and Leuk-A preparations, compared with that of B particles isolated from ICRC milk (Karande et al, 1978) confirms the findings of others (Tanaka 1977, Smith & Wivel, 1973. Recently Tanaka (1977) has analysed polypeptides and antigens of various preparations of Cyt.…”

Section: Electron Microscopysupporting

confidence: 89%

“…Type A particles were isolated and purified from IE2 leukaemic line and mammary tumours of strains ICRC and C3H(Jax) according to the method of Tanaka (1977) which is an improved version of his original method reported in 1972 (Tanaka et al, 1972). B particles were isolated and purified from the milk of ICRC breeders as reported earlier (Karande et al, 1978).…”

Section: Materials Ani) Methodsmentioning

confidence: 99%

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Intracytoplasmic type A particles from mammary tumours and leukaemias of strain ICRC mice

Karande¹,

Joshi²,

Talageri³

et al. 1979

Br J Cancer

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Summary.-The ovarian-hormone-induced leukaemias of strain ICRC mice, with an abundance of intracytoplasmic type A particles in primary as well as transplanted lesions, were used to study morphological, biophysical, immunological and structural characteristics of type A particles. Mammary tumours of strains ICRC and C3H(Jax) were also used as sources for type A particles. The purified virions banded at the density of 1-20 g/ml in 12 60% linear sucrose-density gradient when subjected to spinning at 113,000 g for 4 h. The SDS-polyacrylamide-gel electrophoresis of type A particles from mammary tumours and leukaemias reproducibly resolved at least 8 polypeptides, 2 of these 54,000 and 24,000 dalton proteins, showing variable expression. Type A particles and B particles, despite the fact that each had a distinct polypeptide pattern, showed common antigens with different electrophoretic mobilities. Proteins of 24,000, 18,000 and 12,000 daltons from B particles were found to be antigenically related to those from type A particles. The bioassay studies carried out with purified A particles showed that 2/7 males of strain ICRC and 1/6 females of strain DBA-MTI developed leukaemias, as against none in the controls, when inoculated between the ages of 1-7 days. Spleen tumour and cervical tumour were seen in one female each of strain DBA-MTI.

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Section: Electron Microscopysupporting

confidence: 89%