Characterisation of major vault protein during the life cycle of the human parasite Schistosoma mansoni

Reis, Eneida V.; Pereira, Roberta Verciano; Gomes, Matheus de Souza; Jannotti-Passos, Liana K.; Baba, Elio H.; Coelho, Paulo Marcos Zech; Mattos, Ana Carolina Alves de; Couto, Flávia Fernanda Búbula; Castro-Borges, William; Guerra‐Sá, Renata

doi:10.1016/j.parint.2013.10.005

Cited by 5 publications

(2 citation statements)

References 37 publications

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“…Although their exact function remains unclear, it may be associated with multidrug resistance phenotypes and with signal transduction. In S. mansoni , up-regulation of major vault protein has been observed during the transition from cercaria to schistosomulum and in praziquantel-resistant adult worms [ 35 ]. ATP-binding cassette transporters (ABC transporters) are essential components of cellular physiological machinery, and some ABC transporters, including P-glycoproteins, pump toxins and xenobiotics out of the cell.…”

Section: Resultsmentioning

confidence: 99%

Comparative genomics and transcriptomics of 4 Paragonimus species provide insights into lung fluke parasitism and pathogenesis

et al. 2020

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Background Paragonimus spp. (lung flukes) are among the most injurious foodborne helminths, infecting ∼23 million people and subjecting ∼292 million to infection risk. Paragonimiasis is acquired from infected undercooked crustaceans and primarily affects the lungs but often causes lesions elsewhere including the brain. The disease is easily mistaken for tuberculosis owing to similar pulmonary symptoms, and accordingly, diagnostics are in demand. Results We assembled, annotated, and compared draft genomes of 4 prevalent and distinct Paragonimus species: Paragonimus miyazakii, Paragonimus westermani, Paragonimus kellicotti, and Paragonimus heterotremus. Genomes ranged from 697 to 923 Mb, included 12,072–12,853 genes, and were 71.6–90.1% complete according to BUSCO. Orthologous group analysis spanning 21 species (lung, liver, and blood flukes, additional platyhelminths, and hosts) provided insights into lung fluke biology. We identified 256 lung fluke–specific and conserved orthologous groups with consistent transcriptional adult-stage Paragonimus expression profiles and enriched for iron acquisition, immune modulation, and other parasite functions. Previously identified Paragonimus diagnostic antigens were matched to genes, providing an opportunity to optimize and ensure pan-Paragonimus reactivity for diagnostic assays. Conclusions This report provides advances in molecular understanding of Paragonimus and underpins future studies into the biology, evolution, and pathogenesis of Paragonimus and related foodborne flukes. We anticipate that these novel genomic and transcriptomic resources will be invaluable for future lung fluke research.

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Section: Resultsmentioning

confidence: 99%

Comparative genomics and transcriptomics of 4 Paragonimus species provide insights into lung fluke parasitism and pathogenesis

et al. 2020

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“…Histones H3 have been identified as a biomarker for neoblasts in E. multilocularis metacestode (34). Several identified proteins were previously characterized as antigenic or involved in mechanisms of host immune evasion, such as Antigen B (AgB) (35), tegumental protein (TegP) (36), and putative major vault protein (37). Early studies have proposed that protective antibodies in serum may only be effective on the very early stages of H. taeniaeformis (38,39).…”

Section: Resultsmentioning

confidence: 99%

Proteomics Analysis of Hydatigera taeniaeformis Metacestode Stage

Guo

2020

Front. Vet. Sci.

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Hydatigera taeniaeformis (H. taeniaeformis) is one of the most robust of tapeworm parasites that is widely distributed around the world. Information of proteins of H. taeniaeformis has not previously been reported. Using liquid chromatography tandem-mass spectrometry (LC-MS/MS) analysis, the proteome of H. taeniaeformis metacestode was profiled and a total of 408 proteins were identified. Of these, 26.5% (108/408) were annotated to be associated with metabolic pathways. Consistently, Gene Ontology analysis showed that those identified proteins were mainly classified into metabolic process of the biological processes. A set of metabolic enzymes, including Fructose-1,6-bisphosphate aldolase, enolase, Glucan phosphorylase, and phosphoenolpyruvate carboxykinase, were abundant in H. taeniaeformis metacestodes. In addition, some rare but interesting proteins like antigens (such as tegument protein and Antigen B) were identified. The two recombinant proteins of tegument protein and Antigen B were well-recognized by the sera from the H. taeniaeformis-infected mice. The H. taeniaeformis metacestode proteome might help to find new candidates for the immunodiagnosis and vaccine development and provide valuable information on H. taeniaeformis biology.

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Effects of dietary intake of garlic on intestinal trematodes

et al. 2017

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The current strategy for the control of helminth infections relies on chemotherapy. However, resistance appearance is promoting the necessity of developing new drugs against trematodes. Herein, potential trematocidal effects of garlic (Allium sativum) are investigated in the context of intestinal foodborne trematodes, employing the Echinostoma caproni-mouse model. Daily administration of dietary doses of garlic was conducted in three groups of mice: (i) before infection (prophylaxis), (ii) after infection (therapeutic) and (iii) both, before and after infection (continuous). A fourth group of mice, not exposed to garlic, was used as control. No differences in worm recovery, fecundity and local cytokine expression profiles were found with respect to control infections. However, considerable alterations in tegument structure, including swelling, furrowing, vacuolization and changes in secretory bodies were detected in garlic-exposed parasites using scanning and transmission electron microscopy. Protein secretion was markedly reduced in response to garlic, whereas up-regulation of several proteins, such as major vault protein and tER-ATPase, was observed in treated worms. The results presented herein provide new insights in the anthelminthic activity of bioactive garlic compounds and the manner that parasites respond to toxins.

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Characterisation of major vault protein during the life cycle of the human parasite Schistosoma mansoni

Cited by 5 publications

References 37 publications

Comparative genomics and transcriptomics of 4 Paragonimus species provide insights into lung fluke parasitism and pathogenesis

Comparative genomics and transcriptomics of 4 Paragonimus species provide insights into lung fluke parasitism and pathogenesis

Proteomics Analysis of Hydatigera taeniaeformis Metacestode Stage

Effects of dietary intake of garlic on intestinal trematodes

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