Characterisation of immunodominant protein encoded by the F1L gene of orf virus strains isolated in Italy

Scagliarini, Alessandra; Ciulli, Sara; Battilani, Mara; Jacoboni, I.; Montesi, Francesco; Casadio, Rita; Prosperi, Santino

doi:10.1007/s00705-002-0850-2

Cited by 18 publications

(14 citation statements)

References 19 publications

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“…This approach is being refined to identify protective viral antigens and their genes. The identification of such genes as well as those encoding the immuno dominant proteins from the ORFV may lead to the successful development of subunit vaccines [147][148][149]. There is a potential need to develop new generation or subunit vaccines, which would help to reduce environmental contamination and would be particularly useful in disease-free zones or in flocks with no history of orf outbreaks.…”

Section: Vaccines and Vaccinationmentioning

confidence: 98%

Orf: an update on current research and future perspectives

Hosamani¹,

Scagliarini²,

Bhanuprakash³

et al. 2009

Expert Review of Anti-infective Therapy

157

241

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Orf is one of the most widespread viral diseases worldwide, affecting mostly small ruminants and, sometimes, other species, including wild animals. Of late, there have been an increasing number of reports of new species being affected by the disease, implying a dynamic host-pathogen interaction. The causative agent, orf virus, has been extensively investigated over recent years, owing to its zoonotic importance and ability to cross-infect other species sporadically. The evasive mechanisms that the virus has developed to adapt and grow in the presence of an active immune response helps to explain the ability of the virus to repeatedly reinfect the same host. The apparent diversity in the antigenic/immune targets of different orf virus strains involved in such repeat infections may also be contributing factors. Exposure of animals to stress or immunosupression as a result of therapy or primary viral infection can accentuate the severity of disease. Genes homologous to host cytokines or their antagonists, and which contribute to viral virulence, have been found in the viral genome. A combination of electron microscopy, histology and PCR is the most accurate laboratory approach for confirmation of the disease, although clinical signs are often typical. However, some infections may be confounded by similar clinical manifestations caused by other infections. This review presents, in brief, a recent understanding of the virus at the host-pathogen level, molecular biology of the virus, disease epidemiology, clinical manifestations in man and animals, diagnostic procedures, and the economic and environmental impact of the disease.

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Section: Vaccines and Vaccinationmentioning

confidence: 98%

Orf: an update on current research and future perspectives

Hosamani¹,

Scagliarini²,

Bhanuprakash³

et al. 2009

Expert Review of Anti-infective Therapy

157

241

View full text Add to dashboard Cite

show abstract

“…Transmembrane (TM) domains are indicated by a bold line above the ORFV sequence or below the VACV sequence. The TM domains for F1L and VACV H3L have been reported previously (Lin et al, 2000;Scagliarini et al, 2002). Secondary structure predictions are denoted by '$' (a-helices) or ';' (b-sheets).…”

Section: Prediction Of Secondary Structure and Topology Of Orfv Strucmentioning

confidence: 99%

“…10 kDa and F1L are major proteins of the virion (Czerny et al, 1997;Housawi et al, 1998;Scagliarini, et al, 2002;Spehner et al, 2004) and are homologous with the MV envelope-associated proteins of VACV A27L and H3L, respectively. ORF-110 is a homologue of VACV A34R and is one of few ORFV proteins predicted to be associated with the outer envelope of EV particles.…”

Section: Prediction Of Secondary Structure and Topology Of Orfv Strucmentioning

confidence: 99%

“…The ORFV 10 kDa protein is essential for the formation of an intact surface tubule that wraps the virus particle (Spehner et al, 2004), but it is not known whether it is incorporated into the surface tubule. ORFV F1L contains heparin-binding domains and has been implicated in adsorption of the virus to cells during infection (Scagliarini et al, 2002). In addition, F1L is a major immunodominant antigen (Czerny et al, 1997;Housawi et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

“…The structural proteins of ORFV that have been most studied, the 10 kDa protein (ORF-104) (Naase et al, 1991;Spehner et al, 2004) and F1L (ORF-059) (Housawi et al, 1998;Scagliarini et al, 2002Scagliarini et al, , 2004, are homologues of VACV A27L and H3L, respectively. Both A27L and H3L are expressed late during virus replication and are associated with the membrane envelope of the MV form.…”

Section: Introductionmentioning

confidence: 99%

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Investigation of orf virus structure and morphogenesis using recombinants expressing FLAG-tagged envelope structural proteins: evidence for wrapped virus particles and egress from infected cells

Tan

Ueda

Mercer

et al. 2009

Journal of General Virology

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Orf virus (ORFV) is the type species of the genus Parapoxvirus, but little is known about the structure or morphogenesis of the virus. In contrast, the structure and morphogenesis of vaccinia virus (VACV) has been extensively studied. VACV has two main infectious forms, mature virion (MV) and extracellular virion (EV). The MV is wrapped by two additional membranes derived from the trans-Golgi to produce a wrapped virion (WV), the outermost of which is lost by cellular membrane fusion during viral egress to form the EV. Genome sequencing of ORFV has revealed that it has homologues of almost all of the VACV structural genes. Notable exceptions are A36R, K2L, A56R and B5R, which are associated with WV and EV envelopes. This study investigated the morphogenesis and structure of ORFV by fusing FLAG peptide to the structural proteins 10 kDa, F1L and ORF-110 to form recombinant viruses. 10 kDa and F1L are homologues of VACV A27L and H3L MV membrane proteins, whilst ORF-110 is homologous to VACV A34R, an EV membrane protein. Immunogold labelling of FLAG proteins on virus particles isolated from lysed cells showed that FLAG-F1L and FLAG-10 kDa were displayed on the surface of infectious particles, whereas ORF-110-FLAG could not be detected. Western blot analysis of solubilized recombinant ORF-110-FLAG particles revealed that ORF-110-FLAG was abundant and undergoes post-translational modification indicative of endoplasmic reticulum trafficking. Fluorescent microscopy confirmed the prediction that ORF-110-FLAG localized to the Golgi in virus-infected cells. Finally, immunogold labelling of EVs showed that ORF-110-FLAG became exposed on the surface of EV-like particles as a result of egress from the cell.

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Genus Parapoxvirus

Fleming¹,

Mercer²

2007

Poxviruses

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Characterisation of immunodominant protein encoded by the F1L gene of orf virus strains isolated in Italy

Cited by 18 publications

References 19 publications

Orf: an update on current research and future perspectives

Orf: an update on current research and future perspectives

Investigation of orf virus structure and morphogenesis using recombinants expressing FLAG-tagged envelope structural proteins: evidence for wrapped virus particles and egress from infected cells

Genus Parapoxvirus

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