Characterisation of immune responses and protective efficacy in mice after immunisation with Rift Valley Fever virus cDNA constructs

Lagerqvist, Nina; Näslund, Jonas; Lundkvist, Åke; Bouloy, Michèle; Ahlm, Clas; Bucht, Gösta

doi:10.1186/1743-422x-6-6

Cited by 65 publications

(60 citation statements)

References 38 publications

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“…The nucleocapsid (N) protein of RVFV encapsidates the three genome segments of the virus (the L, M, and S segments). It is considered a major immunogen and immunizations using the N antigen have been shown to evoke partial protective immunity in animal models [24][25][26]. The Gn and Gc glycoproteins protrudes from the surface of the virions and mediate attachment to the cell receptor [27].…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis thaliana plants expressing Rift Valley fever virus antigens: Mice exhibit systemic immune responses as the result of oral administration of the transgenic plants

Kalbina

Lagerqvist

Moiane

et al. 2016

Protein Expression and Purification

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Section: Introductionmentioning

confidence: 99%

Arabidopsis thaliana plants expressing Rift Valley fever virus antigens: Mice exhibit systemic immune responses as the result of oral administration of the transgenic plants

Kalbina

Lagerqvist

Moiane

et al. 2016

Protein Expression and Purification

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“…Indeed, vaccination with N antigen has been shown to protect mice from wild-type RVFV challenge (Wallace et al 2006, Liu et al 2008, Lorenzo et al 2008, Lagerqvist et al 2009, Jansen van Vuren et al 2010, Lorenzo et al 2010. Remarkably, the glycoprotein Gne also showed an early-response reactivity (day 3 pv) in western blot and ELISA with MP12-vaccinated sera and was detected by day-28 pi sera from all ZH501-infected sheep.…”

Section: Figmentioning

confidence: 96%

Rift Valley Fever Virus Structural and Nonstructural Proteins: Recombinant Protein Expression and Immunoreactivity Against Antisera from Sheep

Faburay

Wilson²,

McVey³

et al. 2013

Vector-Borne and Zoonotic Diseases

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The Rift Valley fever virus (RVFV) encodes the structural proteins nucleoprotein (N), aminoterminal glycoprotein (Gn), carboxyterminal glycoprotein (Gc), and L protein, 78-kD, and the nonstructural proteins NSm and NSs. Using the baculovirus system, we expressed the full-length coding sequence of N, NSs, NSm, Gc, and the ectodomain of the coding sequence of the Gn glycoprotein derived from the virulent strain of RVFV ZH548. Western blot analysis using anti-His antibodies and monoclonal antibodies against Gn and N confirmed expression of the recombinant proteins, and in vitro biochemical analysis showed that the two glycoproteins, Gn and Gc, were expressed in glycosylated form. Immunoreactivity profiles of the recombinant proteins in western blot and in indirect enzyme-linked immunosorbent assay against a panel of antisera obtained from vaccinated or wild type (RVFV)-challenged sheep confirmed the results obtained with anti-His antibodies and demonstrated the suitability of the baculo-expressed antigens for diagnostic assays. In addition, these recombinant proteins could be valuable for the development of diagnostic methods that differentiate infected from vaccinated animals (DIVA).

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“…2A). While nucleoproteins are not known to be directly involved in pathogenesis, experiments using recombinant N have shown it to elicit a partial degree of immune protection (37,42). Although strongly immunogenic, N cannot elicit neutralizing antibodies.…”

Section: Functional Characterization Of Rvfv Components and Their Rolmentioning

confidence: 99%

“…Although strongly immunogenic, N cannot elicit neutralizing antibodies. Furthermore, it has been shown that RVFV-immunized sheep and mice exhibit lymphocytic proliferation in vitro in the presence of recombinant N protein (37,42,43). This suggests that this level of immunity is mediated through the interactions of antigen-presenting cells (APCs) with T helper cells.…”

Section: Functional Characterization Of Rvfv Components and Their Rolmentioning

confidence: 99%

Rift Valley Fever: Recent Insights into Pathogenesis and Prevention

Boshra¹,

Lorenzo²,

Busquets

et al. 2011

J Virol

108

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2Rift Valley fever virus (RVFV) is a zoonotic pathogen that primarily affects ruminants but can also be lethal in humans. A negative-stranded RNA virus of the family Bunyaviridae, this pathogen is transmitted mainly via mosquito vectors. RVFV has shown the ability to inflict significant damage to livestock and is also a threat to public health. While outbreaks have traditionally occurred in sub-Saharan Africa, recent outbreaks in the Middle East have raised awareness of the potential of this virus to spread to Europe, Asia, and the Americas. Although the virus was initially characterized almost 80 years ago, the only vaccine approved for widespread veterinary use is an attenuated strain that has been associated with significant pathogenic side effects. However, increased understanding of the molecular biology of the virus over the last few years has led to recent advances in vaccine design and has enabled the development of more-potent prophylactic measures to combat infection. In this review, we discuss several aspects of RVFV, with particular emphasis on the molecular components of the virus and their respective roles in pathogenesis and an overview of current vaccine candidates. Progress in understanding the epidemiology of Rift Valley fever has also enabled prediction of potential outbreaks well in advance, thus providing another tool to combat the physical and economic impact of this disease.

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Characterisation of immune responses and protective efficacy in mice after immunisation with Rift Valley Fever virus cDNA constructs

Cited by 65 publications

References 38 publications

Arabidopsis thaliana plants expressing Rift Valley fever virus antigens: Mice exhibit systemic immune responses as the result of oral administration of the transgenic plants

Arabidopsis thaliana plants expressing Rift Valley fever virus antigens: Mice exhibit systemic immune responses as the result of oral administration of the transgenic plants

Rift Valley Fever Virus Structural and Nonstructural Proteins: Recombinant Protein Expression and Immunoreactivity Against Antisera from Sheep

Rift Valley Fever: Recent Insights into Pathogenesis and Prevention

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