Characterisation of<i>Pasteurella multocida</i>isolated from fowl cholera outbreaks on turkey farms

Blackall, P. J.; Pahoff, J.L.; Marks, David H.; Fegan, Narelle; Morrow, Chris J.

doi:10.1111/j.1751-0813.1995.tb15033.x

Cited by 45 publications

(53 citation statements)

References 15 publications

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“…Previous work from our group had indicated that outbreaks of fowl cholera in both turkeys and free-range layers were associated with single clones of P. multocida (Blackall et al, 1995, Zhang et al, 2004. Indeed, in the free-range layer outbreak, two outbreaks separated by 10 years were associated with the same strain (Zhang et al, 2004).…”

Section: Discussionmentioning

confidence: 89%

“…There have been many studies on the epidemiology of fowl cholera outbreaks in a variety of hosts; for example, in turkeys (Snipes et al, 1989(Snipes et al, , 1990Christiansen et al, 1992;Blackall et al, 1995;Kardos & Kiss, 2005), geese (Kardos & Kiss, 2005), free-range layers (Zhang et al, 2004), conventional layer flocks (Shivachandra et al, 2005;Eigaard et al, 2006), backyard poultry (Christensen et al, 1998) and ducks (Muhairwa et al, 2000). Unlike these prior studies, our current study has used both a phenotypic method (Heddleston serotyping) as well as a genotypic method (MLST).…”

Section: Discussionmentioning

confidence: 94%

“…In some studies, there have been multiple genotypes associated with outbreaks in turkeys (Snipes et al, 1989(Snipes et al, , 1990Christiansen et al, 1992). In contrast, other outbreaks have been reported as being associated with a single clone; for example, in turkey flocks (Blackall et al, 1995), in free-range layers (Zhang et al, 2004), in conventional layer flocks (Shivachandra et al, 2005;Eigaard et al, 2006) and in backyard poultry (Christensen et al, 1998). Fewer studies have looked at the persistence of P. multocida isolates in repeated outbreaks over time within the one farm.…”

Section: Introductionmentioning

confidence: 99%

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Studies on the presence and persistence ofPasteurella multocidaserovars and genotypes in fowl cholera outbreaks

Singh

Blackall

Remington³

2013

Avian Pathology

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Pasteurella multocida was isolated from poultry on six farms (one free-range duck farm, one free-range turkey farm, one conventional enclosed turkey farm, and three free-range layer farms) suffering fowl cholera outbreaks. In addition, historical isolates from previous outbreaks were available for the conventional turkey farm and the three free-range layer farms. The isolates were serotyped using the Heddleston scheme and genotyped using multi-locus sequencing typing. In the current outbreaks, two of the farms had two different sequence types (STs) of P. multocida in the investigated outbreak (the free-range turkey farm and one of the free-range layer farms). The remaining four farms had one ST within the investigated outbreak. In looking at the historical isolates, two of the four farms had multiple genotypes involved. On the four farms with historical isolates from previous outbreaks, at least one new genotype was present in the investigated outbreak as compared with the historical isolates. On one layer farm, one genotype persisted over a 10-year period. Serotyping revealed the presence of multiple serovars in the current and historical outbreaks, with serovars sometimes changing over time. This study has shown that several STs of P. multocida can be present during some outbreaks of fowl cholera, although other outbreaks involve a single ST. Also, the STs present on a property suffering repeated fowl cholera can both persist and change over time.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Studies on the presence and persistence ofPasteurella multocidaserovars and genotypes in fowl cholera outbreaks

Singh

Blackall

Remington³

2013

Avian Pathology

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“…The molecular-based techniques of restriction endonuclease analysis and ribotyping have been used extensively to demonstrate considerable diversity in avian isolates of P. multocida (Kim & Nagaraja, 1990;Carpenter et al, 1991;Christiansen et al, 1992a, b;Wilson et al, 1993Wilson et al, , 1995Blackall et al, 1995). While this evidence of diversity is well known, there has been no attempt to assemble an overview of the overall population structure of avian P. multocida isolates in relation to this diversity.…”

Section: Introductionmentioning

confidence: 99%

Population structure and diversity of avian isolates of Pasteurella multocida from Australia

Blackall¹,

Fegan²,

Chew

et al. 1998

Microbiology

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A total of 110 isolates of Pasteurella multocida from Australian poultry and reference strains for the 16 somatic serovars plus the three subspecies (gallicida, multocida, septica) were analysed to examine their population structure and diversity. The 81 field isolates examined by multilocus enzyme electrophoresis (MLEE) were diverse, being divided into 56 electrophoretic types (ETs), with the 19 reference strains in another 15 ETs. The population was clonal and somatic serotyping was not particularly useful in establishing relationships between isolates. The 71 ETs formed three distinct subclusters (A, B and C) at a genetic distance of 036. Biovars tended to be associated with these subclusters: A with biovars 1,3,4, 5 and 8 and B with biovars 2,6,7,9 and 10. Ribotyping, performed on all 110 isolates using Hpall, recognized 21 ribotypes forming nine clusters (Rl-Rg). The isolates in ribotype cluster R 1 were almost identical to those in MLEE cluster B. Using both MLEE and ribotyping, the 19 non-Australian reference strains were found to be distributed over the full diversity of the Australian isolates of P. multocida. This study has shown that a range of P. multocida clones are associated with fowl cholera in Australia and that many of the Australian isolates are similar to non-Australian reference strains. Both the MLEE results and the ribotyping data identified a previously unrecognized subset of P. multocida strains.

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“…Previous epidemiological investigations have demonstrated that P. multocida associated with fowl cholera in single domestic turkey flocks may belong to multiple clones (Snipes et al ., 1989(Snipes et al ., , 1990Christiansen et al ., 1992;Rhoades et al ., 1992), indicating the importance of characterizing multiple isolates from fowl cholera outbreaks to obtain reliable epidemiological information. The association of a single clone with outbreaks of fowl cholera has been reported in turkey (Christiansen et al ., 1992;Blackall et al ., 1995) and layer flocks (Zhang et al ., 2004), but this was based on relatively few isolates. A different clone of P. multocida was found in each of four outbreaks of fowl cholera on a duck farm over a 2-year period (Muhairwa et al ., 2000).…”

Section: Introductionmentioning

confidence: 99%

Clonal stability ofPasteurella multocidain free-range layers affected by fowl cholera

et al. 2006

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Detailed longitudinal studies of the genetic stability of Pasteurella multocida ssp. multocida, the cause of fowl cholera, have not previously been carried out. Consequently, the aim of the present study was to provide detailed information on the genetic stability and diversity of P. multocida ssp. multocida in poultry flocks over time, enabling new insights into the molecular epidemiology of this important poultry pathogen. Longitudinal investigations of the rate and causes of mortality were carried out on two free-range layer farms (A and B) over a period of 11 months. The total mortality of two flocks, A 1 and A 2 , on farm A were 62 and 91%, respectively, while the total mortality of a single flock B 1 on farm B was 6%. Postmortem examinations were performed on 708 layers from flocks A 1 and A 2 and in 159 from flock B 1 . Fowl cholera was the main cause of mortality on both farms. Pasteurella multocida isolates recovered from layers on both farms were characterized phenotypically and genotypically, and 322 isolates were identified as P. multocida ssp. multocida. The genetic diversity of 99 isolates from farm A and 31 from farm B was characterized by restriction endonuclease analysis and amplified fragment length polymorphism analysis. The isolates on each farm had a unique restriction endonuclease analysis and amplified fragment length polymorphism analysis type, suggesting a

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Characterisation ofPasteurella multocidaisolated from fowl cholera outbreaks on turkey farms

Cited by 45 publications

References 15 publications

Studies on the presence and persistence ofPasteurella multocidaserovars and genotypes in fowl cholera outbreaks

Studies on the presence and persistence ofPasteurella multocidaserovars and genotypes in fowl cholera outbreaks

Population structure and diversity of avian isolates of Pasteurella multocida from Australia

Clonal stability ofPasteurella multocidain free-range layers affected by fowl cholera

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