Characterisation of<i>P. falciparum</i>antigenic determinants isolated from a genomic expression library by differential antibody screening

Langsley, Gordon; Scherf, Artur; Mercereau‐Puijalon, Odile; Koenen, Michael; Kahane, Bernard; Mattei, Denise; Guillotte, Micheline; Sibilli, Lisa; Garner, Ian; Müller‐Hill, Benno; Silva, Luis Pereira da

doi:10.1093/nar/13.11.4191

Cited by 12 publications

(1 citation statement)

References 20 publications

(17 reference statements)

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“…Where the antigen concerned is available in quantity, monospecific (but polyclonal) antibody may be selected from the serum by affinity purification and then tested in, for instance, a functional assay. A number of antigens (and their genes) have been identified in this way (see, for instance Langsley, Scherf, Mercereau-Puijalon, Koenen, Kahane, Mattei, Guillotte, Sibilli, Garner, Muller-Hill & Periera da Silva, 1985;Kemp, Coppel, Stahl, Bianco, Corcoran, Mclntyre, Langford, Favaloro, Crewther, Brown, Mitchell, Culvenor & Anders, 1986). This and a parallel approach in which the initial identification of the antigen was on fixed and dried blood films by IFA have led to the identification of Pfl55 or RESA (ring-infected erythrocyte surface antigen) (Perlmann, Berzins, Wahlgren, Carlsson, Bjorkman, Wahlin, Wahlgren, Perlmann, Berzins, Bjorkman, Patarroyo & Perlmann, 1984), which is one of the candidate antigens considered in more detail below.…”

Section: Choice Of Antigen By Analysis Of Natural or Induced Antibodymentioning

confidence: 99%

An update on candidate malaria vaccines

Mitchell

1989

Parasitology

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The purpose of this work is to review the progress towards malaria vaccination that has been made over the last four or so years, and the prospects and difficulties as they now appear. Although some of the older literature will be referred to as necessary background, it is not treated here in any detail. The reader who wishes for a fuller historical perspective should see, for instance, Brown (1969), Cohen & Mitchell (1978), Desowitz & Miller (1980), Mitchell (1984), Miller, David & Hadley (1984), Heidrich (1986) and, specifically for a consideration of sporozoite vaccination, Nussenzweig & Nussenzweig (1984, 1986). Naturally, any summary of vaccination will lean on the immunology of the disease, but malaria immunology is not reviewed here in its own right. The reader requiring the most recent attempt to cover this field should see the work edited by Perlmann & Wigzell (1988); some individual chapters of that volume will be referred to below.

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Section: Choice Of Antigen By Analysis Of Natural or Induced Antibodymentioning

confidence: 99%

An update on candidate malaria vaccines

Mitchell

1989

Parasitology

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Cross‐reaction of antibodies to the nine‐amino acid repeats of Plasmodium falciparum antigen 11.1 with human serum albumin

1992

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Mice immunized with the recombinant antigen 11.1 beta-galactosidase, consisting of 22 repeats of the nine-amino acid unit from Plasmodium falciparum antigen 11.1, produced antibodies reacting with human serum albumin. A positive reaction was observed in dot-blot assays, in enzyme-linked immunosorbent assay and on immunoblots of sodium dodecyl sulfate polyacrylamide gels as well as two-dimensional gels. Binding was specific for human albumin, as no reaction could be detected on bovine serum albumin, hen egg ovalbumin, rat serum albumin or another abundant human serum protein, the alpha 2-macroglobulin. In addition, rabbit antibodies raised to human serum albumin reacted with keyhole lympet hemocyanin coupled to synthetic dimers of the nine-amino acid repeats of the P. falciparum 11.1 antigen. These data indicate antigenic relationship between the 11.1 antigen and human albumin. The proteins have a short sequence of homology in a region where human serum albumin differs from the albumins of other species.

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Specific and nonspecific immunodiagnostic properties of recombinant and syntheticPlasmodium falciparum antigens

Knobloch

Schreiber

Grokhovsky³

et al. 1987

Eur. J, Clin. Microbiol.

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Six Plasmodium falciparum/beta-galactosidase fusion proteins produced by a genomic DNA expression library, and two synthetic Plasmodium falciparum antigens were applied to ELISA and tested for their immunodiagnostic properties. Results were compared to reference methods, i.e. fluorescence antibody test with whole cell antigen and ELISA with detergent-soluble crude schizont antigen. Anti-Plasmodium falciparum antibodies could be detected by these molecular antigens to varying extents in human sera. Undesired reactivity to fusion proteins due to the high prevalence of antibodies to beta-galactosidase in human sera and undesired reactivity to one of the synthetic antigens (P12) frequently occurred. The antibodies responsible for the nonspecific reactivity could not be identified. It was concluded that the application of molecular Plasmodium falciparum antigens to ELISA represents a practicable approach to immunodiagnosis of malaria if the construction of epitopes that bind antibodies other than Plasmodium falciparum antibodies can be avoided.

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Characterisation ofP. falciparumantigenic determinants isolated from a genomic expression library by differential antibody screening

Cited by 12 publications

References 20 publications

An update on candidate malaria vaccines

An update on candidate malaria vaccines

Cross‐reaction of antibodies to the nine‐amino acid repeats of Plasmodium falciparum antigen 11.1 with human serum albumin

Specific and nonspecific immunodiagnostic properties of recombinant and syntheticPlasmodium falciparum antigens

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