Characterisation of humoral immune responses in dogs vaccinated with irradiated Ancylostoma caninum

Boag, Peter R.; Parsons, Jim C.; Presidente, P. J. A.; Spithill, Terry W.; Sexton, Julianne

doi:10.1016/s0165-2427(03)00006-0

Cited by 36 publications

(27 citation statements)

References 20 publications

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“…In addition, for humans and mice, IgG1 and IgG2 subtypes have been traditionally used as surrogates of the Th2 and Th1 phenotypes of immune responses respectively. For dogs, although the association between IgG subtypes and the immune response phenotype were not demonstrated yet, experimental evidences indicate that this association should occur [4,11,38].…”

Section: Discussionmentioning

confidence: 98%

Immunogenicity in dogs of three recombinant antigens (TSA, LeIF and LmSTI1) potential vaccine candidates for canine visceral leishmaniasis

et al. 2005

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-Control of canine visceral leishmaniasis (VL) remains a difficult and serious problem mostly because there is no reliable and effective vaccine available to prevent this disease. A mixture of three recombinant leishmanial antigens (TSA, LeIF and LmSTI1) encoded by three genes highly conserved in the Leishmania genus have been shown to induce excellent protection against infection in both murine and simian models of cutaneous leishmaniasis. A human clinical trial with these antigens is currently underway. Because of the high degree of conservation, these antigens might be useful vaccine candidates for VL as well. In the present study, using the dog model of the visceral disease, we evaluated the immunogenicity of these three antigens formulated with two different adjuvants, MPL-SE ® and AdjuPrime ® . The results were compared with a whole parasite vaccine formulated with BCG as the adjuvant. In order to investigate if sensitization with the recombinant antigens would result in recognition of the corresponding native parasite antigens upon infection, the animals were exposed for four weeks after the termination of the immunization protocol with the recombinant antigens to a low number of L. chagasi promastigotes, an etiological agent of VL. Immune response was evaluated by quantitative ELISA in the animal sera before and after exposure

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Section: Discussionmentioning

confidence: 98%

Immunogenicity in dogs of three recombinant antigens (TSA, LeIF and LmSTI1) potential vaccine candidates for canine visceral leishmaniasis

et al. 2005

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“…Freitas (1977) and Barriga (1991) proposed that development of adult larvae is rare in dogs older than six months of age, because most larvae tend to undergo somatic migration and develop into a latent state. The estrus phase in female dogs may reactivate these larvae and transmit them to fetuses and newborns through the placenta and milk (BOWMAN et al, 2010). This transmission cycle may explain the high incidence of Toxocara in this study, since the majority of dogs (73.3%) were less than six months old.…”

Section: Resultsmentioning

confidence: 78%

“…These results are different from those reported by Pimpão et al (2005), who observed good efficacy against gastrointestinal nematodes in dogs who received 600 µg/kg intradermally. Bowman et al (2010) also reported high efficacy of this drug at a dosage of 0.2 mg/kg.…”

Section: Fecrt (%) G1 Fecrt (%) G2mentioning

confidence: 84%

“…These finding are similar to those reported by Fisher (2003) However, some studies have reported Ancylostoma to be more prevalent than Toxocara in dog excrement samples, including Kopp et al (2007), who analyzed animal between three and six months of age; Lorenzini et al (2007) who studied animals one year of age; and Prates et al (2009), Ramirez-Barrios et al (2004, and Swai et al (2010), who analyzed dogs in different age ranges. Dogs of all ages can be infected by A. caninum because they do not develop effective immunity against this helminth (BLAZIUS et al, 2005;BOAG et al, 2003).…”

Section: Resultsmentioning

confidence: 99%

“…Gastroenteritis may occur in dogs of all ages, but is most common in neonates and animals less than six months of age, primarily due to their immature immune systems and transmammary and transplacental transmission (BOWMAN et al, 2010;RAMIREZ-BARRIOS et al, 2004 HOPKINS et al, 1998;KOPP et al, 2007).…”

Section: Among Infections Common In Dogsmentioning

confidence: 99%

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Efficacy of pyrantel pamoate and ivermectin for the treatment of canine nematodes

et al. 2015

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This study evaluated the efficacy of pyrantel pamoate and ivermectin on gastrointestinal nematodes in dogs. Fecal egg counts per gram (EPG) were measured by the fecal egg count reduction test (FECRT) in order to evaluate the anthelmintic efficiency and fecal float exams were also performed to assess the concordance between coproparasitological techniques. A total of 45 naturally infected dogs in the city of Bandeirantes, Paraná State, were selected and divided into three groups: Group 1, 15 animals that received pyrantel pamoate (145 mg) in a single dose; Group 2, 15 animals that received ivermectin (3 mg); and Group 3, 15 animals that comprised an untreated control group. Fecal testing was performed two and 10 days after treatment. Toxocara was the most prevalent genus, followed by Ancylostoma and Trichuris. Ancylostoma had low resistance to ivermectin and pyrantel pamoate treatment, while Toxocara were resistant to both treatments. Statistical correlation testing to compare coproparasitogical techniques revealed moderate concordance, substantial and almost perfect concordance for detection of Ancylostoma, Trichuris, and Toxocara, respectively. The results of this study suggest that the gender Ancylostoma had low resistance and Toxocara is resistant to both drugs and because of their high prevalence in young animals means that others anthelmintic drugs may be recommended to combat infections. Additionally, the Gordon and Whitlock modified and Willis-Mollay techniques are effective for detection particularly of Toxocara in dogs. Key words: Ancylostomiasis, dogs, parasitic resistance, toxocariasis ResumoObjetivou-se neste estudo avaliar a eficácia do pamoato pirantel e da ivermectina sobre nematódeos gastrointestinais de cães. Para tanto foram realizados a contagem de ovos nas fezes (OPG) para avaliação da eficiência anti-helmíntica através do Teste de Redução da Contagem de Ovos nas Fezes (RCOF) e exame de flutuação Willis-Mollay para verificação de concordância entre as técnicas coproparasitológicas. Selecionou-se 45 cães na cidade de Bandeirantes, Paraná, naturalmente infectados, os quais foram divididos em três grupos experimentais: Grupo 1, 15 animais que receberam pamoato de pirantel 145 mg; Grupo 2, 15 animais que receberam ivermectina 3 mg e Grupo 3, 15 animais do grupo controle, não tratados. Coletas de fezes foram realizadas dois e dez dias depois para realização dos exames coproparasitológicos. Observou-se que o gênero de maior prevalência foi Toxocara, seguido de Ancylostoma e Trichuris. Ancylostoma apresentou baixa resistência ao tratamento

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Vaccines Linked to Chemotherapy: A New Approach to Control Helminth Infections

Lustigman¹,

McKerrow²,

Bottazzi³

2012

Parasitic Helminths

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Characterisation of humoral immune responses in dogs vaccinated with irradiated Ancylostoma caninum

Cited by 36 publications

References 20 publications

Immunogenicity in dogs of three recombinant antigens (TSA, LeIF and LmSTI1) potential vaccine candidates for canine visceral leishmaniasis

Immunogenicity in dogs of three recombinant antigens (TSA, LeIF and LmSTI1) potential vaccine candidates for canine visceral leishmaniasis

Efficacy of pyrantel pamoate and ivermectin for the treatment of canine nematodes

Vaccines Linked to Chemotherapy: A New Approach to Control Helminth Infections

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