Characterisation of genomic RNA of Coxsackievirus B3 in murine myocarditis: reliability of direct sequencing of reverse transcription-nested polymerase chain reaction products

Zhang, Hongyi; Soteriou, B.; Knowlson, Sarah; Theodoridou, Agapi; Archard, L. C.

doi:10.1016/s0166-0934(97)00122-5

Cited by 8 publications

(7 citation statements)

References 43 publications

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“…As a result of this technique, the enteroviral genomic RNA was detected in samples of patients with infectious heart diseases (9,14). However, when a low copy number of viruses is present in the samples, the RT-PCR may fail to produce enough amplified products (amplicons) to be either detected by the ethidium bromide staining or used for further molecular manipulation (35). …”

Section: Introductionmentioning

confidence: 99%

Coxsackievirus B detection in cases of myocarditis, myopericarditis, pericarditis and dilated cardiomyopathy in hospitalized patients

Gaâloul

Riabi

Harrath

et al. 2014

Molecular Medicine Reports

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Coxsackieviruses B (CV-B) are known as the most common viral cause of human heart infections. The aim of the present study was to assess the potential role of CV-B in the etiology of infectious heart disease in hospitalized patients. The present study is based on blood, pericardial fluid and heart biopsies from 102 patients and 100 control subjects. All of the samples were examined for the detection of specific enteroviral genome using the reverse transcription polymerase chain reaction (RT-PCR) and sequence analysis. Immunohistochemical investigations for the detection of the enteroviral capsid protein, VP1, from the biopsies were performed. The samples were cultured on confluent KB monolayer cell line for possible virus isolation. The epidemiological data were also collected. CV-B was detected in 28 of the 102 patients. The sequence analysis demonstrated that 27 strains were identical to CV-B3 and only one strain was identical to CV-B1. Furthermore, VP1 in the heart biopsies was detected in enterovirus-positive cases, as revealed by RT-PCR. Pericarditis infection was more frequent than myocarditis (P<0.05) or myopericarditis (P=0.05). The epidemiological data demonstrate that CV-B heart infections occur mainly during autumn and winter, and young male adults are more susceptible than adolescents or adults (P<0.5). The present findings demonstrate a higher prevalence of viral heart infections, suggesting that CV-B may significantly contribute to heart infections.

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Section: Introductionmentioning

confidence: 99%

Coxsackievirus B detection in cases of myocarditis, myopericarditis, pericarditis and dilated cardiomyopathy in hospitalized patients

Gaâloul

Riabi

Harrath

et al. 2014

Molecular Medicine Reports

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“…To establish the aetiological role of enteroviruses in Keshan disease and to characterise the genomic sequence of the viruses involved, we conducted a multicentre study using an established RT-PCR technique coupled with direct nucleotide sequencing. The reliability of these techniques has been evaluated in an experimental model of coxsackievirus B3 myocarditis20and used successfully in our laboratory to identify enterovirus involved in human heart muscle disease 21…”

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confidence: 99%

High prevalence of enteroviral genomic sequences in myocardium from cases of endemic cardiomyopathy (Keshan disease) in China

Peng²,

Yang³

et al. 2000

Heart

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Objective-To verify the aetiological involvement of enterovirus and identify the viral genomic sequences in Keshan disease. Design-Formalin fixed, paraYn embedded myocardial necropsy tissue samples were collected in Keshan disease endemic regions. Fourteen cases with a histologically confirmed diagnosis of subacute or chronic Keshan disease were studied. Control tissue included 10 samples of myocardium from cases of cerebral trauma and one from accidental acid intoxication. One sample from a case of enteroviral myocarditis was used as a positive control. The presence of viral genomic RNA was investigated using an established reverse transcription nested polymerase chain reaction (PCR) coupled with direct nucleotide sequencing. Further investigations of PCR positive samples included in situ antigen detection or hybridisation to confirm positive results. Results-Nine of 14 myocardial samples from Keshan disease cases and the positive control were positive for the enteroviral RNA. All the controls were negative. Six of the PCR positive samples were investigated further by in situ enteroviral antigen or RNA detection and all were positive. DNA sequencing of six representative PCR products confirmed that they were homologous to the 5' non-translated region of enteroviral genomic RNA. Five had highest homology to coxsackievirus B genotypes and one was identical to poliovirus type 3. Conclusions-These results support an aetiological role for enteroviral infection in Keshan disease. Nucleotide sequence data suggest that coxsackievirus B or coxsackie B like viruses are often involved in Keshan disease. (Heart 2000;83:696-701)

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“…• The efficacy of the poliovirus vaccine suggests that an enteroviral vaccine or a coxsackievirus-specific vaccine may reduce the incidence of myocarditis or DCM. Zhang et al [28] reported that immunization with an attenuated variant of coxsackievirus B3 protected mice from challenge with a cardiovirulent strain. The attenuated strain neither infected the myocardium nor induced antimyosin antibodies.…”

Section: Immunomodulatory Therapy (Intravenous Immunoglobulin)mentioning

confidence: 99%

Myocarditis

Winkel¹,

Parrillo²

2002

Curr Treat Options Cardio Med

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Myocarditis and its sequela, dilated cardiomyopathy (DCM), cause substantial morbidity and mortality, especially in children and young adults. Physicians should include myocarditis in the differential diagnosis of all patients who have new symptoms of heart failure, arrhythmia, or chest pain syndromes of unclear cause, and should strongly consider performing endomyocardial biopsy (EMB) to establish the diagnosis. It may be necessary to perform multiple or serial biopsies to increase sensitivity. Patients with myocarditis and symptomatic heart failure, chest pain, or arrhythmias need hospitalization for evaluation and treatment. Patients with symptomatic left ventricular dysfunction should be treated with conventional heart failure therapy, including angiotensin-converting enzyme (ACE) inhibitors, digitalis, diuretics, and beta-blockers. Patients with arrhythmias or syncope may require electrophysiologic evaluation. In addition to conventional therapy, physicians should consider a course of immunosuppressive therapy in selected patients. The clinical course, response to therapy, and left ventricular function need close monitoring. Patients with myocarditis and rapidly progressive heart failure or cardiogenic shock should be referred early to an advanced heart failure center for implantation of a ventricular assist device and consideration for cardiac transplantation.

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Characterisation of genomic RNA of Coxsackievirus B3 in murine myocarditis: reliability of direct sequencing of reverse transcription-nested polymerase chain reaction products

Cited by 8 publications

References 43 publications

Coxsackievirus B detection in cases of myocarditis, myopericarditis, pericarditis and dilated cardiomyopathy in hospitalized patients

Coxsackievirus B detection in cases of myocarditis, myopericarditis, pericarditis and dilated cardiomyopathy in hospitalized patients

High prevalence of enteroviral genomic sequences in myocardium from cases of endemic cardiomyopathy (Keshan disease) in China

Myocarditis

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