Characterisation of genes involved in galactolipids and sulfolipids metabolism in maize and Arabidopsis and their differential responses to phosphate deficiency

Wang, Rui; Ding, Dong; Li, Jiaxin; Xu, Xiaoxuan; Ying, Zhao; Yan, Bowei; Li, Zuotong; Xu, Jingyu

doi:10.1071/fp19082

Cited by 20 publications

(14 citation statements)

References 41 publications

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“…In this study, a significant decrease of MGDG and a slight increase of DGDG was observed, resulting in a large reduction in the MGDG/DGDG ratio in maize seedlings under cold stress. MGD catalyzes the galactose transfer from UDP-galactose to DAG framework to form MGDG, and then the second galactose is diverted from UDP-galactose to MGDG by DGD for the final formation of DGDG (Wang et al, 2020). In our transcriptomic data, the expression of two maize DGDs were up-regulated, and the most apparent one had a Log2FC of 2.56.…”

Section: Discussionmentioning

confidence: 60%

Membrane Lipids’ Metabolism and Transcriptional Regulation in Maize Roots Under Cold Stress

et al. 2021

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Low temperature is one of the major abiotic stresses that restrict the growth and development of maize seedlings. Membrane lipid metabolism and remodeling are key strategies for plants to cope with temperature stresses. In this study, an integrated lipidomic and transcriptomic analysis was performed to explore the metabolic changes of membrane lipids in the roots of maize seedlings under cold stress (5°C). The results revealed that major extraplastidic phospholipids [phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA), and phosphatidylinositol (PI)] were dominant membrane lipids in maize root tissues, accounting for more than 70% of the total lipids. In the transcriptome data of maize roots under cold stress, a total of 189 lipid-related differentially expressed genes (DEGs) were annotated and classified into various lipid metabolism pathways, and most of the DEGs were enriched in the “Eukaryotic phospholipid synthesis” (12%), “Fatty acid elongation” (12%), and “Phospholipid signaling” (13%) pathways. Under low temperature stress, the molar percentage of the most abundant phospholipid PC decreased around 10%. The significantly up-regulated expression of genes encoding phospholipase [phospholipase D (PLD)] and phosphatase PAP/LPP genes implied that PC turnover was triggered by cold stress mainly via the PLD pathway. Consequently, as the central product of PC turnover, the level of PA increased drastically (63.2%) compared with the control. The gene-metabolite network and co-expression network were constructed with the prominent lipid-related DEGs to illustrate the modular regulation of metabolic changes of membrane lipids. This study will help to explicate membrane lipid remodeling and the molecular regulation mechanism in field crops encountering low temperature stress.

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Section: Discussionmentioning

confidence: 60%

Membrane Lipids’ Metabolism and Transcriptional Regulation in Maize Roots Under Cold Stress

et al. 2021

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“…This membrane remodeling occurs in a process dependent on the activation of monogalactosyldiacylglycerol synthase induced by a low Pi level. Studies have already observed this pattern in leaves and roots with different plant species under Pi depletion, including Sesamum indicum , Zea mays , and Arabidopsis thaliana [ 42 , 45 , 46 ].…”

Section: Discussionmentioning

confidence: 88%

“…Phosphorus deficiency in canga can be related to the formation of complexes between P and iron oxides, limiting plant uptake availability [ 41 ]. Roots from plants sampled in canga showed more proteins related to P depletion, such as monogalactosyldiacylglycerol synthase 2, phospholipase, alcohol dehydrogenase, extracellular purple acid phosphatases, and antioxidant system proteins such as catalases and monodehydroascorbate reductases [ 42 , 43 , 44 ]. During Pi deprivation, glycolipids are transferred to extraplastid membranes, where they replace degraded phospholipids to meet the need for Pi essential for various biological processes in the cell.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Profiling and Rhizosphere-Associated Microbial Communities Reveal Adaptive Mechanisms of Dioclea apurensis Kunth in Eastern Amazon’s Rehabilitating Minelands

Nascimento

Costa

Herrera

et al. 2022

Plants

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Dioclea apurensis Kunth is native to ferruginous rocky outcrops (known as canga) in the eastern Amazon. Native cangas are considered hotspots of biological diversity and have one of the largest iron ore deposits in the world. There, D. apurensis can grow in post-mining areas where molecular mechanisms and rhizospheric interactions with soil microorganisms are expected to contribute to their establishment in rehabilitating minelands (RM). In this study, we compare the root proteomic profile and rhizosphere-associated bacterial and fungal communities of D. apurensis growing in canga and RM to characterize the main mechanisms that allow the growth and establishment in post-mining areas. The results showed that proteins involved in response to oxidative stress, drought, excess of iron, and phosphorus deficiency showed higher levels in canga and, therefore, helped explain its high establishment rates in RM. Rhizospheric selectivity of microorganisms was more evident in canga. The microbial community structure was mostly different between the two habitats, denoting that despite having its preferences, D. apurensis can associate with beneficial soil microorganisms without specificity. Therefore, its good performance in RM can also be improved or attributed to its ability to cope with beneficial soil-borne microorganisms. Native plants with such adaptations must be used to enhance the rehabilitation process.

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“…This membrane remodeling occurs in a process dependent on the activation of monogalactosyldiacylglycerol synthase induced by a low Pi level. Studies have already observed this pattern in leaves and roots with different plant species under Pi depletion, including Sesamum indicum, Zea mays, and Arabidopsis thaliana [46,49,50].…”

Section: Proteins Involved In the Response To P-starvationmentioning

confidence: 71%

“…Phosphorus deficiency in canga can be related to the formation of complexes between P and iron oxides, limiting plant uptake availability [45]. Roots from plants sampled in canga showed more proteins related to P depletion, such as monogalactosyldiacylglycerol synthase 2, phospholipase, alcohol dehydrogenase, extracellular purple acid phosphatases, and antioxidant system proteins such as catalases and monodehydroascorbate reductases [46][47][48]. During Pi deprivation, glycolipids are transferred to extraplastid membranes, where they replace degraded phospholipids to meet the need for Pi essential for various biological processes in the cell.…”

Section: Proteins Involved In the Response To P-starvationmentioning

confidence: 99%

Proteomic Profiling and Rhizosphere-Associated Microbial Communities Reveal Adaptive Mechanisms of Dioclea Apurensis Kunth in Eastern Amazon’s Rehabilitating Minelands

Nascimento¹,

Costa²,

Herrera³

et al. 2022

Preprint

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Dioclea apurensis Kunth is native to ferruginous rocky outcrops (known as canga) in the eastern Amazon. Native cangas are considered hotspots of biological diversity and have one of the largest iron ore deposits in the world. There, D. apurensis can grow in post-mining areas where molecular mechanisms and rhizospheric interactions with soil microorganisms are expected to contribute to their establishment in rehabilitating minelands. In this study, we compare the root proteomic profile and rhizosphere-associated bacterial and fungal communities of D. apurensis growing in canga and a rehabilitating mineland to characterize the main mechanisms that allow the growth and establishment in post-mining areas. The results showed that proteins involved in response to oxidative stress, drought, excess of iron, and phosphorus deficiency were more accumulated in canga and, therefore, helped explain its high establishment rates in rehabilitating minelands. Rhizospheric selectivity of microorganisms was more evident in canga. The microbial community structure was mostly different between the two habitats, denoting that despite having its preferences, D. apurensis can associate with beneficial soil microorganisms without specificity. Therefore, its good performance in rehabilitating minelands can also be improved or attributed to its ability to cope with beneficial soil-borne microorganisms. Native plants with such adaptations must be used to enhance the rehabilitation process.

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Characterisation of genes involved in galactolipids and sulfolipids metabolism in maize and Arabidopsis and their differential responses to phosphate deficiency

Cited by 20 publications

References 41 publications

Membrane Lipids’ Metabolism and Transcriptional Regulation in Maize Roots Under Cold Stress

Membrane Lipids’ Metabolism and Transcriptional Regulation in Maize Roots Under Cold Stress

Proteomic Profiling and Rhizosphere-Associated Microbial Communities Reveal Adaptive Mechanisms of Dioclea apurensis Kunth in Eastern Amazon’s Rehabilitating Minelands

Proteomic Profiling and Rhizosphere-Associated Microbial Communities Reveal Adaptive Mechanisms of Dioclea Apurensis Kunth in Eastern Amazon’s Rehabilitating Minelands

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