Characterisation of Fungi of Stored Common Bean Cultivars Grown in Menoua Division, Cameroon

Akwa, Teh Exodus; Maingi, John M.; Birgen, Jonah

doi:10.1101/2020.10.31.363184

Cited by 2 publications

(2 citation statements)

References 30 publications

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“…The ITS gene amplification followed the procedure by Akwa et al ( 2020 ). Primer pairs used were: 5′-TCCGTAGGTGAACCTGCGG-3′ and 5′-TCCGTAGGTGAACCTGCGG-3′.…”

Section: Methodsmentioning

confidence: 99%

Analysis of the bacterial and fungal populations in South African sorghum beer (umqombothi) using full-length 16S rRNA amplicon sequencing

Hlangwani,

Abrahams,

Masenya

et al. 2023

World J Microbiol Biotechnol

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There is a need to profile microorganisms which exist pre-and-post-production of umqombothi, to understand its microbial diversity and the interactions which subsequently influence the final product. Thus, this study sought to determine the relative microbial abundance in umqombothi and predict the functional pathways of bacterial and fungal microbiota present. Full-length bacterial 16S rRNA and internal transcribed spacer (ITS) gene sequencing using PacBio single-molecule, real-time (SMRT) technology was used to assess the microbial compositions. PICRUSt2 was adopted to infer microbial functional differences. A mixture of harmful and beneficial microorganisms was observed in all samples. The microbial diversity differed significantly between the mixed raw ingredients (MRI), customary beer brew (CB), and optimised beer brew (OPB). The highest bacterial species diversity was observed in the MRI, while the highest fungal species diversity was observed in the OPB. The dominant bacterial species in the MRI, CB, and OPB were Kosakonia cowanii, Apilactobacillus pseudoficulneus, and Vibrio alginolyticus, respectively, while the dominant fungal species was Apiotrichum laibachii. The predicted functional annotations revealed significant (p < 0.05) differences in the microbial pathways of the fermented and unfermented samples. The most abundant pathways in the MRI were the branched-chain amino acid biosynthesis super pathway and the pentose phosphate pathway. The CB sample was characterised by folate (vitamin B9) transformations III, and mixed acid fermentation. Biotin (vitamin B7) biosynthesis I and l-valine biosynthesis characterised the OPB sample. These findings can assist in identifying potential starter cultures for the commercial production of umqombothi. Specifically, A. pseudoficulneus can be used for controlled fermentation during the production of umqombothi. Likewise, the use of A. laibachii can allow for better control over the fermentation kinetics such as carbohydrate conversion and end-product characteristics, especially esters and aroma compounds.

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“…The ITS gene amplification followed the procedure by Akwa et al ( 2020 ). Primer pairs used were: 5′-TCCGTAGGTGAACCTGCGG-3′ and 5′-TCCGTAGGTGAACCTGCGG-3′.…”

Section: Methodsmentioning

confidence: 99%

Analysis of the bacterial and fungal populations in South African sorghum beer (umqombothi) using full-length 16S rRNA amplicon sequencing

Hlangwani,

Abrahams,

Masenya

et al. 2023

World J Microbiol Biotechnol

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“…Preventive chemical control and qualified (registered and certified) seed use are also considered. Fusarium control is an important issue due to some fungal species on stored common bean can also release mycotoxins (fumonisin) which causes human mycotoxicoses upon consumption, esophageal cancer and interferes with sphingolipid metabolism [18]. Fusarium -An Overview on Current Status of the Genus…”

Section: Fusarium Natural Incidencementioning

confidence: 99%

Importance of the Natural Incidence of the Fusarium Genus in Food Crops Established in Northern México

Saucedo¹,

Ramírez-Valadez²,

Espinoza³

et al. 2022

Fusarium - An Overview of the Genus

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The incidence of the Fusarium genus causing root rot is reviewed in crops showing high importance for food supply and to obtain regular income by farmers in the highlands of Northern México. Pathogen incidence was evaluated under field conditions in multiple sampling locations for common beans (Phaseolus vulgaris L.) and several chili peppers (Capsicum annuum) local cultivars (landraces and bred cultivars). Five commercial plots for registered and certified seed were also evaluated in common beans to be used in the ‘seed refreshing program’ implemented for the cultivar Pinto Saltillo, considered as the main variety sown in the highlands of México. High Fusarium genus incidence and its interactions with other fungi species, such as Rhizoctonia solani and Pythium spp., cause high losses in plant population, commercial yield and seed quality in food crops grown in Northern México. The natural incidence of plant disease caused by the Fusarium genus and its negative effect on crop survival and the reduction of commercial yield and seed quality is fully reviewed. Plant disease resistance, crop breeding and the influence of the environmental conditions were also considered.

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Characterisation of Fungi of Stored Common Bean Cultivars Grown in Menoua Division, Cameroon

Cited by 2 publications

References 30 publications

Analysis of the bacterial and fungal populations in South African sorghum beer (umqombothi) using full-length 16S rRNA amplicon sequencing

Analysis of the bacterial and fungal populations in South African sorghum beer (umqombothi) using full-length 16S rRNA amplicon sequencing

Importance of the Natural Incidence of the Fusarium Genus in Food Crops Established in Northern México

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