Characterisation of Four Species of the Genus Kluyveromyces by Mitochondrial DNA Restriction Analysis

Belloch, Carmela; Barrio, Eladio; Uruburu, F.; García, María del Mar Román; Querol, Amparo

doi:10.1016/s0723-2020(97)80008-2

Cited by 36 publications

(31 citation statements)

References 27 publications

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“…The members of this significant group are highly related and appear clearly separated from the other Kluyveromyces species. This is consistent with the ability of these strains to mate in the laboratory (Johannsen, 1980) and with the results of DNA base composition and relatedness (Fuson et al, 1987 ;Vaughan-Martini & Martini, 1987), isoenzyme analysis (Sidenberg & Lachance, 1983 and karyotyping (Sor & Fukuhara, 1989 ;Viljoen et al, 1989), although there is evidence of heterogeneity in these species at the intraspecific level (Cottrell et al, 1987 ;Belloch et al, 1997 ;Lehmann et al, 1992 ;Molnar et al, 1996). A second group includes the species K. africanus, Kluyveromyces delphensis, Kluyveromyces lodderae, K. polysporus and Kluyveromyces yarrowii, which appear intermixed with species belonging to the genera Saccharomyces, Torulaspora and Zygosaccharomyces in a polyphyletic group.…”

Section: Belloch and Otherssupporting

confidence: 84%

Phylogeny of the genus Kluyveromyces inferred from the mitochondrial cytochrome-c oxidase II gene.

Belloch¹,

Querol²,

García³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

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A phylogenetic analysis of 17 species belonging to the genus Kluyveromyces and 12 reference and outgroup species was performed using mitochondrial cytochrome-c oxidase II gene sequences. The genus Kluyveromyces appears as a polyphyletic taxon formed by species included within the following four main groups. The Kluyveromyces phaffii group encompasses the species Kluyveromyces blattae, K. phaffii and Kluyveromyces yarrowii. The Kluyveromyces marxianus group is a monophyletic group consisting of the species Kluyveromyces aestuarii, Kluyveromyces dobzhanskii, Kluyveromyces lactis, K. marxianus and Kluyveromyces wickerhamii. The monophyletic Kluyveromyces thermotolerans group is formed by K. thermotolerans, Kluyveromyces waltii and Saccharomyces kluyveri (which appears in the mitochondrial tree as the sister clade of the K. marxianus group). Finally, the Saccharomyces cerevisiae group contains the remaining Kluyveromyces species, as well as the reference Saccharomyces species (sensu lato and sensu stricto) and Candida glabrata (the phylogenetic relationships within this group are unclear according to the bootstrap test). The phylogenetic relationships obtained for this mitochondrial gene are, for the most part, congruent with previous trees based on nuclear rRNA sequences, except for the position of K. yarrowii and the close relationship between the K. marxianus and K. thermotolerans groups. These differences, as well as the existence of these groups, are discussed in the context of previous studies based on phenotypic, genetic and molecular data. Although additional studies are required to decipher the phylogenetic relationships between the genus Kluyveromyces and the closely related genera Saccharomyces, Torulaspora and Zygosaccharomyces, future changes to their taxonomic status should take account of the existence of these four groups of Kluyveromyces species.

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Section: Belloch and Otherssupporting

confidence: 84%

Phylogeny of the genus Kluyveromyces inferred from the mitochondrial cytochrome-c oxidase II gene.

Belloch¹,

Querol²,

García³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

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“…drosophilarum vary more in their karyotype patterns. Similar results are also drawn from the analysis of mtDNA restriction patterns (Ragnini and Fukuhara, 1988;Belloch et al, 1997).…”

Section: Yeastsupporting

confidence: 80%

“…Thus, mitochondrial DNA restriction analysis (Belloch et al, 1997) indicated a high level of polymorphism within K. lactis, which supported the subdivision into varieties. In this way, the type strain of K. phaseolosporus (var.…”

Section: Genetic Polymorphism Within K Lactismentioning

confidence: 61%

“…The closeness among these species has also been demonstrated by mating studies (Johannsen, 1980;Naumov, 1986Naumov, , 1988, DNA base composition and hybridization (Fuson et al, 1987;Vaughan-Martini and Martini, 1987), isoenzyme analysis Lachance, 1983, 1986), karyotypes (Sor and Fukuhara, 1989;Viljoen et al, 1989;Lehmann et al, 1992;Belloch et al, 1998b), restriction analysis of mtDNA (Ragnini and Fukuhara, 1988;Belloch et al, 1997), and RAPD-PCR analysis (Molnar et al, 1996). However, some of these phylogenetic relationships obtained with different molecular markers are elusive or even contradictory, especially those involving the species K. dobzhanskii, K. lactis and K. marxianus.…”

Section: Introductionmentioning

confidence: 94%

“…Additional studies involving electrophoretic isoenzyme pattern analysis (Sidenberg and Lachance, 1986), deoxyribonucleic acid base sequence relatedness (Fuson et al, 1987;Vaughan-Martini and Martini, 1987), chromosomal karyotyping (Sor and Fukuhara, 1989;Belloch et al, 1998b), RAPD-PCR analysis (Molnar et al, 1996) and mtDNA restriction analysis (Ragnini and Fukuhara, 1988;Belloch et al, 1997) were puzzling because they were indicative of the existence of genetic heterogeneity within K. lactis, supporting the differentiation between the varieties lactis and drosophilarum but, at the same time, a marked variation is also observed within varieties as well.…”

Section: Genetic Polymorphism Within K Lactismentioning

confidence: 99%

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An analysis of inter- and intraspecific genetic variabilities in the Kluyveromyces marxianus group of yeast species for the reconsideration of the K. lactis taxon

Belloch¹,

Fernández-Espinar²,

Querol³

et al. 2002

Yeast

Self Cite

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In the present work, we analyse the sequences of the 5.8S rRNA gene and the two internal transcribed spacers 1 and 2 (5.8S-ITS region), obtained from 39 strains belonging to the species Kluyveromyces aestuarii, K. dobzhanskii, K. lactis and K. marxianus, K. nonfermentans and K. wickerhamii, to solve the phylogenetic relationships among these species and also to determine the possible genetic basis for the delimitation of the two currently accepted K. lactis varieties: lactis, including lactose-positive strains isolated from dairy products, and drosophilarum, comprising lactose-negative strains isolated from insects and plant exudates. The determination of the phylogenetic relationships within the species K. lactis, together with the examination of the electrophoretic karyotypes and phenotypic characterization of strains representatives of K. lactis var. lactis and var. drosophilarum, allowed differentiation of two groups of strains. The first, and ancestral, group comprises lactose-negative strains isolated from natural habitats in North America. The second, and derived, group includes both lactose-negative strains isolated from natural habitats in Europe and wine fermentation in South Africa, and lactose-positive strains associated with dairy products. These results suggest that the present taxon K. lactis is a complex of different species, subspecies or, at least, genetically structured populations.

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Inter- and intraspecific chromosome pattern variation in the yeast genusKluyveromyces

Belloch

Barrio

García

et al. 1998

Yeast

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The analysis of the electrophoretic chromosome patterns of the species of the genus Kluyveromyces, reveals a high polymorphism in size, number and intensity of bands. Different sets of electrophoresis running conditions were used to establish species‐specific patterns and also to detect intraspecific variation. According to their karyotypes, the species of this genus can be divided into two major groups. The first group includes the species K. africanus, K. bacillisporus, K. delphensis, K. lodderae, K. phaffi, K. polysporus and K. yarrowii, composing the so‐called ‘Saccharomyces cerevisiae‐like’ group, because their karyotypes resemble that of the species S. cerevisiae. The second group comprises the species K. aestuarii, K. blattae, K. dobzhanskii, K. lactis, K. marxianus, K. thermotolerans, K. waltii and K. wickerhamii, whose chromosomal patterns exhibit common characteristics very different to those of the species included in the ‘S. cerevisiae‐like’ group. This division is concordant with the position of these species in previous phylogenetic reconstructions. Additionally, the intraspecific analysis of the chromosome patterns show a rich polymorphism in the heterogeneous species K. dobzhanskii, K. lactis, and K. marxianus, which is in concordance with the variability observed with other phenotypic or genetic markers. On the contrary, K. thermotolerans exhibits a homogeneous karyotype indicative of a very low level of chromosomal polymorphism, which is congruent with the reduced variability found in this species with other molecular markers. © 1998 John Wiley & Sons, Ltd.

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Characterisation of Four Species of the Genus Kluyveromyces by Mitochondrial DNA Restriction Analysis

Cited by 36 publications

References 27 publications

Phylogeny of the genus Kluyveromyces inferred from the mitochondrial cytochrome-c oxidase II gene.

Phylogeny of the genus Kluyveromyces inferred from the mitochondrial cytochrome-c oxidase II gene.

An analysis of inter- and intraspecific genetic variabilities in the Kluyveromyces marxianus group of yeast species for the reconsideration of the K. lactis taxon

Inter- and intraspecific chromosome pattern variation in the yeast genusKluyveromyces

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