Characterisation of excitatory and inhibitory transmitter systems in prostate glands of rats, guinea pigs, rabbits and pigs

Najbar-Kaszkiel, Alicja T; Iulio, Juliana L Di; Li, Chun Guang; Rand, M. J.

doi:10.1016/s0014-2999(97)01270-3

Cited by 32 publications

(29 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, in our laboratory and in other recent studies, it has been found that atropine reduced ®eld stimulation-induced contractions of the guinea-pig prostate, (Haynes & Hill, 1997;Najbar-Kaszkiel et al, 1997;. In addition, we have observed that anticholinesterases produced a marked atropine-sensitive but hexamethoniuminsensitive enhancement of ®eld stimulation-induced contractions of prostate smooth muscle in this species .…”

Section: Introductionsupporting

confidence: 59%

“…For these and other reasons including the fact that the guinea-pig prostate exhibits gonadal steroid sensitivity similar to that of the human (Maini et al, 1997), the guinea-pig prostate may provide a useful animal model for studies in the human. This possibility is reinforced by the ®nding that neuromuscular transmission to the smooth muscle of the guinea-pig prostate, as to that in the human prostate, is predominantly sympathetic and noradrenergic in nature (Ohkawa 1983;Haynes & Hill, 1997;Najbar-Kaszkiel et al, 1997;.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cholinergic facilitation of neurotransmission to the smooth muscle of the guinea‐pig prostate gland

Lau

Pennefather

Mitchelson

2000

British J Pharmacology

View full text Add to dashboard Cite

1 Functional experiments have been conducted to assess the eects of acetylcholine and carbachol, and the receptors on which they act to facilitate neurotransmission to the stromal smooth muscle of the prostate gland of the guinea-pig. 2 Acetylcholine and carbachol (0.1 mM ± 0.1 mM) enhanced contractions evoked by trains of electrical ®eld stimulation (20 pulses of 0.5 ms at 10 Hz every 50 s with a dial setting of 60 V) of nerve terminals within the guinea-pig isolated prostate. In these concentrations they had negligible eects on prostatic smooth muscle tone. The facilitatory eects of acetylcholine, but not those of carbachol, were further enhanced in the presence of physostigmine (10 mM). 3 The facilitatory eects of carbachol were unaected by the neuropeptide Y Y 1 receptor antagonist BIBP 3226 ((R)-N 2 -(diphenylacetyl)-N-[(4-hydroxyphenyl)methyl]-argininamide) (0.3 mM, n=3) or suramin (100 mM, n=5). Prazosin (0.1 mM, n=5) and guanethidine (10 mM, n=5) alone and in combination (n=4), reduced responses to ®eld stimulation and produced rightward shifts of the log concentration-response curves to carbachol. 4 The rank orders of potency of subtype-preferring muscarinic receptor antagonists in inhibiting the facilitatory actions of acetylcholine and carbachol were: pirenzepine 4 HHSiD (hexahydrosiladifenidol) 4 pF-HHSiD (para-¯uoro-hexahydrosiladifenidol) 5 himbacine, and pirenzepine 4 HHSiD 4 himbacine 5 pF-HHSiD, respectively. These pro®les suggest that muscarinic receptors of the M 1 -subtype mediate the facilitatory eects of acetylcholine and carbachol on neurotransmission to the smooth muscle of the guinea-pig prostate.

show abstract

Section: Introductionsupporting

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Cholinergic facilitation of neurotransmission to the smooth muscle of the guinea‐pig prostate gland

Lau

Pennefather

Mitchelson

2000

British J Pharmacology

View full text Add to dashboard Cite

show abstract

“…20,21 Electrical field stimulation of nerves within human, rat, guinea-pig, rabbit, pig and dog prostate causes contractions that can be abolished by tetrodotoxin, reduced by guanethidine and blocked by ␣ 1 -adrenoceptor antagonists. 19,[21][22][23] These studies indicate that there are similar mechanisms at play in the prostates of all mammalian species and, therefore, that there may be advantages in using animal prostate models to investigate human prostate function. These advantages include the ready availability of animal tissue and the fact that the tissue obtained from laboratory animals is, by nature, very consistent and, in contrast with human tissue obtained at surgery, complete and free from disease.…”

Section: Animal Prostate Models Of Human Prostate Contractilitymentioning

confidence: 99%

“…Muscarinic receptors are present in the prostate of all species investigated and both muscarinic agonists and/or the stimulation of cholinergic nerves will produce contractions in isolated tissues taken from rats, pigs and guinea-pigs, 19,22,46 as well as from certain regions of the dog and rabbit prostate. 47,48 The effects of muscarinic agonists in human prostate are equivocal; they have been shown to potentiate responses to noradrenaline, but not to cause contraction, 9 and they have been shown to elicit contractions in their own right.…”

Section: Animal Prostate Models Of Human Prostate Contractilitymentioning

confidence: 99%

“…The human prostate shows considerable nitrergic innervation 52 and nitric oxide has a powerful inhibitory effect on contractility in prostates taken from all animals studied, including humans. 15,22 However, marked species differences have been reported in the extent of nitrergic activity; thus, rabbit, human and dog prostates appear to have a significant non-adrenergic, non-cholinergic inhibitory activity that is not evident in rat, guineapig or pig prostates. 15,22,53 In terms of prostate models, it appears that, in this instance, the rabbit is the preferred model of prostate activity because it suffers a functional loss of nitrergic innervation associated with age, as is seen in human prostate.…”

Section: Animal Prostate Models Of Human Prostate Contractilitymentioning

confidence: 99%

See 1 more Smart Citation

Current models of human prostate contractility

Haynes

Ventura

2005

Clin Exp Pharma Physio

View full text Add to dashboard Cite

SUMMARY1. The human prostate is a compact gland contributing to seminal fluid. With increasing age, most humans will develop benign prostatic hyperplasia, a condition of prostatic enlargement and contractility that leads to occlusion of the urethra. Over many years, investigators have used a variety of animal and cell culture models to elucidate some of the contractile and proliferative mechanisms that may be associated with the development of this condition.2. This review briefly assesses the current state of knowledge of the mechanisms underlying human prostatic contractility and compares it with that of animal and cell culture models. It is not intended as a comprehensive methodological review, nor is it intended to indicate our preferences for either model. Our aim is to correlate findings from animal and cell culture models with the current understanding of human prostate contractility.3. We hope that the present review will increase awareness of the suitability of the current models in developing our understanding of benign prostatic hyperplasia.

show abstract

Clinical and experimental aspects of Adreno‐muscarinic synergy in the bladder base and prostate

Roosen

Blake-James

Wood

et al. 2009

Neurourology and Urodynamics

View full text Add to dashboard Cite

Recent clinical trials have shown that combination therapy using an alpha-receptor antagonist and an antimuscarinic is more effective than either agent alone in improving quality of life and objective urodynamic variables in men with bladder outflow obstruction. There appear to be no negative effects on bladder function. The mode of action of this combination is unknown but presumed to be an antimuscarinic reduction in detrusor overactivity and the alpha-receptor antagonist reduced outflow tract resistance. We have shown with in vitro experiments that in smooth muscles influencing outflow tract resistance (prostate, trigone) there is a profound contractile synergy between adrenergic and muscarinic pathways. We propose the hypothesis that both arms of the combination therapy reduce contractile tone of the outflow tract and that their simultaneous attenuation has a disproportionately large effect on outflow tract resistance. Our data from trigone muscle suggest that adrenergic and muscarinic receptor activation increase the intracellular [Ca(2+)] but the adrenergic pathway also operates through Ca(2+)-sensitisation of the contractile apparatus, primarily through a PKC-dependent pathway.

show abstract

Characterisation of excitatory and inhibitory transmitter systems in prostate glands of rats, guinea pigs, rabbits and pigs

Cited by 32 publications

References 29 publications

Cholinergic facilitation of neurotransmission to the smooth muscle of the guinea‐pig prostate gland

Cholinergic facilitation of neurotransmission to the smooth muscle of the guinea‐pig prostate gland

Current models of human prostate contractility

Clinical and experimental aspects of Adreno‐muscarinic synergy in the bladder base and prostate

Contact Info

Product

Resources

About