Characterisation of Escherichia fergusonii isolates from farm animals using an Escherichia coli virulence gene array and tissue culture adherence assays

Wragg, Peter; Ragione, Roberto M. La; Best, Angus; Reichel, R.; Anjum, Muna F.; Mafura, Muriel; Woodward, Martin J.

doi:10.1016/j.rvsc.2008.05.014

Cited by 34 publications

(34 citation statements)

References 35 publications

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“…Among the members of Enterobacteriaceae, E. fergusonii has a distinct lactose-nonfermenting phenotype closest to that of E. coli. There are few reports of clinically significant E. fergusonii human and animal infections, suggesting that this organism may cause enteric infections in different hosts (1,8,16,21,23). Interestingly, we have encountered a high frequency of multidrug-resistant E. fergusonii in fecal samples of clinically sick pigs in our laboratory and at the National Veterinary Research and Quarantine Service (NVRQS) of South Korea since 2007.…”

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confidence: 80%

“…Several recent studies have reported that Escherichia fergusonii, a member of the Enterobacteriaceae, is becoming resistant to the available antimicrobial therapy options (2,17,23). Among the members of Enterobacteriaceae, E. fergusonii has a distinct lactose-nonfermenting phenotype closest to that of E. coli.…”

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confidence: 99%

“…Because E. fergusonii was previously misidentified as a variant biotype of E. coli O157:H7 by Vitek, and to determine if some of the isolates carried E. coli O157 somatic antigens, 20 randomly selected E. fergusonii isolates were checked for cross-reaction with antisera specific for E. coli O antigens 157 and 55 at the NVRQS of South Korea (6,7,23).…”

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confidence: 99%

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Plasmid Typing and Resistance Profiling of Escherichia fergusonii and Other Enterobacteriaceae Isolates from South Korean Farm Animals

Rayamajhi

Bin

Shin

et al. 2011

Appl Environ Microbiol

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In this study, we focused on determining the distribution and prevalence of major plasmid replicons in ␤-lactam-resistant Escherichia fergusonii and Enterobacteriaceae of animal and human origin. A high degree of plasmid variability and multiple plasmid replicons were observed among the isolates. The IncF and IncI1 replicons were the most prevalent in E. fergusonii and Salmonella enterica serovar Indiana isolated from swine and poultry in South Korea, respectively. The presence of broad-host-range plasmid replicons such as IncN, IncA/C, IncHI1, and IncHI2 that are associated with important virulence genes and toxins as well as antimicrobial resistance determinants indicates that E. fergusonii has the potential to become an important pig pathogen and possible emerging opportunistic zoonotic pathogen.

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Plasmid Typing and Resistance Profiling of Escherichia fergusonii and Other Enterobacteriaceae Isolates from South Korean Farm Animals

Rayamajhi

Bin

Shin

et al. 2011

Appl Environ Microbiol

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“…The rectal swab samples were inoculated into 5 ml of tryptic soy broth (Merck Millipore ® , Germany) and incubated at 37 °C for 24 h. Ten microlitres of each culture in TSB were inoculated onto Simmons citrate agar (Oxoid ® , UK) containing 4% adonitol (SCA) as described before (Foster et al 2010) and eosin methylene blue agar for identification of E. coli. The agar plates were incubated at 37 °C for 24 h. Adonitol-positive yellow colonies were transferred to sorbitol MacConkey agar (SMAC) and incubated at 37 °C for 24 h (Wragg et al 2009;Oh et al 2012). After Gram staining, Gram-negative small coccobacilli were subjected to further identification.…”

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confidence: 99%

Molecular identification and antimicrobial resistence of Escherichia fergusonii and Escherichia coli from dairy cattle with diarrhoea

Parın¹,

Kırkan²,

Arslan³

et al. 2018

Vet. Med. - Czech

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ABSTRACT:The aim of this study was to determine the incidence of Escherichia fergusonii in dairy cattle with clinical signs of diarrhoea. The specimens were obtained from three different farms in Denizli province of Turkey, between August 2016 and December 2016. Rectal contents of 57 Holstein-friesian dairy cattle with diarrhoea were collected from farms located in the Aegean Region (Denizli province, Turkey). Rectal swabs were inoculated into enrichment, differential and selective culture media. A total of 49 (86%) Escherichia spp. were isolated by phenotypic identification from 57 rectal swab samples. Presumptive E. fergusonii isolates were tested with the API 20E identification kit and all isolates (100%) were identified as E. coli. Primers targeting specific E. coli and E. fergusonii and genes, including the beta-glucuronidase enzyme, conserved hypothetical cellulose synthase protein and regulator of cellulose synthase and hypothetical protein, putative transcriptional activator for multiple antibiotic resistance were used for detection and differentiation of E. coli and E. fergusonii. Thirteen of the 49 E. coli-verified isolates were identified as E. fergusonii after duplex PCR using EFER 13-and EFER YP-specific primers. Confirmation of strain identity was conducted using Sanger sequencing analysis. The rates of antibiotic resistance of E. fergusonii to penicillin G and erythromycin were 100% and 77%, respectively. In conclusion, field strains of E. fergusonii were detected in cattle with diarrhoea in Turkey, and the strains were found to be resistant to multiple antibiotics.

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“…In animals, E. fergusonii has been isolated from pigs, sheep, cattle, goats, horses, reindeer, ostriches, turkeys, and chickens displaying symptoms of salmonellosis-like infections, including diarrhea as well as mastitis, meningitis, abortion, and septicemia (2)(3)(4)(5)(6). This bacterium also has been isolated from human blood, urine, feces, spinal fluid, and, most often, wound exudates from people with conditions such as sepsis, urinary tract infections, enteric diseases, pancreatic carcinoma, and wound infection (2,3,6,7). Several virulence factors, including the presence of a heat-labile toxin on a plasmid, are involved in the pathogenesis of E. fergusonii (2,5,8).…”

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Duplex PCR Methods for the Molecular Detection of Escherichia fergusonii Isolates from Broiler Chickens

Simmons

Rempel

Block

et al. 2014

Appl Environ Microbiol

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e Escherichia fergusonii is an emerging pathogen that has been isolated from a wide range of infections in animals and humans. Primers targeting specific genes, including yliE (encoding a conserved hypothetical protein of the cellulose synthase and regulator of cellulose synthase island), EFER_1569 (encoding a hypothetical protein, putative transcriptional activator for multiple antibiotic resistance), and EFER_3126 (encoding a putative triphosphoribosyl-dephospho-coenzyme A [CoA]), were designed for the detection of E. fergusonii by conventional and real-time PCR methods. Primers were screened by in silico PCR against 489 bacterial genomic sequences and by both PCR methods on 55 reference and field strains. Both methods were specific and sensitive for E. fergusonii, showing amplification only for this bacterium. Conventional PCR required a minimum bacterial concentration of approximately 10 2 CFU/ml, while real-time PCR required a minimum of 0.3 pg of DNA for consistent detection. Standard curves showed an efficiency of 98.5%, with an R 2 value of 0.99 for the real-time PCR assay. Cecal and cloacal contents from 580 chickens were sampled from broiler farms located in the Fraser Valley (British Columbia, Canada). Presumptive E. fergusonii isolates were recovered by enrichment and plating on differential and selective media. Of 301 total presumptive isolates, 140 (46.5%) were identified as E. fergusonii by biochemical profiling with the API 20E system and 268 (89.0%) using PCR methods. E. fergusonii detection directly from cecal and cloacal samples without preenrichment was achieved with both PCR methods. Hence, the PCR methods developed in this work significantly improve the detection of E. fergusonii.

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Characterisation of Escherichia fergusonii isolates from farm animals using an Escherichia coli virulence gene array and tissue culture adherence assays

Cited by 34 publications

References 35 publications

Plasmid Typing and Resistance Profiling of Escherichia fergusonii and Other Enterobacteriaceae Isolates from South Korean Farm Animals

Plasmid Typing and Resistance Profiling of Escherichia fergusonii and Other Enterobacteriaceae Isolates from South Korean Farm Animals

Molecular identification and antimicrobial resistence of Escherichia fergusonii and Escherichia coli from dairy cattle with diarrhoea

Duplex PCR Methods for the Molecular Detection of Escherichia fergusonii Isolates from Broiler Chickens

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