Characterisation of eleven new polymorphic microsatellite markers for the coastal stingray Dasyatis brevicaudata (Dasyatidae Hutton 1875), and cross-amplification in seven dasyatid species

Port, Agnès Le; Roycroft, Emily; Thakur, Vibha; Lavery, Shane

doi:10.1016/j.bse.2016.02.009

Cited by 3 publications

(3 citation statements)

References 17 publications

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“…In addition to the haplotype analysis, 244 samples were genotyped with nuclear microsatellites. Primer pairs of previously published microsatellite markers for stingrays were tested (Anderson, 2017; Le Port et al ., 2016). A fluorescent‐dye labelled M13‐tail (5'‐CACGACGTTGTAAAACGA‐3′) was added to either the forward or reverse primer sequence of each originally published primer pair.…”

Section: Methodsmentioning

confidence: 99%

“…PCR amplification with the primers from Anderson (2017) was conducted with an initial denaturation of 5:00 min at 95°C, followed by 40 cycles of 95°C for 00:30 min, annealing on specific annealing temperature for 00:30 min and an extension at 72°C for 00:30 min followed by a final extension at 72°C for 10:00 min. The PCR amplification with other primers was conducted according to the given protocol (Le Port et al ., 2016). The microsatellite fragments were genotyped through polyacrylamide gel electrophoresis on a LI‐COR 4300 DNA Analyser using the SAGA GT (LI‐COR) software and visual confirmation.…”

Section: Methodsmentioning

confidence: 99%

“…In addition to the haplotype analysis, 244 samples were gen- (Anderson, 2017;Le Port et al, 2016). A fluorescent-dye labelled M13-tail (5'-CACGACGTTGTAAAACGA-3 0 ) was added to either the forward or reverse primer sequence of each originally published primer pair.…”

Section: Dna Extraction and Genotypingmentioning

confidence: 99%

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Linking local movement and molecular analysis to explore philopatry and population connectivity of the southern stingray Hypanus americanus

Schwanck

Schweinsberg

Lampert

et al. 2020

Journal of Fish Biology

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Limited data pertaining to life history and population connectivity of the datadeficient southern stingray (Hypanus americanus) are available. To determine potential vulnerabilities of their populations, this study aimed to analyse their movement patterns and genetic variability. A population of southern stingrays encompassing nine sites around Cape Eleuthera, the Bahamas, has been monitored using markrecapture, spanning a 2.5 year period. Out of 200 individual stingrays, more than a third were encountered again. The home range of the females appears to be restricted, which supports the notion of high site residency. As resident populations of stingrays could suffer from a lack of population connectivity and be predestined for genetic isolation and local extirpation, this study further investigated the genetic connectivity of four sample sites in the central and western Bahamas. A haplotype analysis from the mitochondrial D-loop region showed that no distinct population structure strictly correlated with the sample site. These findings were complemented by five microsatellite loci that revealed high degrees in genotypic variability and little population differentiation. The results suggest gene flow mediated by both males and females.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Linking local movement and molecular analysis to explore philopatry and population connectivity of the southern stingray Hypanus americanus

Schwanck

Schweinsberg

Lampert

et al. 2020

Journal of Fish Biology

View full text Add to dashboard Cite

show abstract

Population structure and male-biased dispersal in the short-tail stingray Bathytoshia brevicaudata (Myliobatoidei: Dasyatidae)

2019

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Genetic diversity assessment for the vulnerable migratory cownose ray Rhinoptera bonasus (Myliobatiformes: Rhinopteridae) from the southwestern Atlantic Ocean

et al. 2021

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Rhinoptera bonasus is a bento-pelagic and highly migratory species occurring from southern United States to northern Argentina. Due to overfishing effects, R. bonasus is currently at risk, classified by the IUCN Red List as vulnerable. Considering the lack of molecular data available for R. bonasus, this study aimed to describe the genetic variability and population structure of specimens sampled from three Brazilian coast ecoregions (Amazon ecoregion, Pará; Northeastern ecoregion, Pernambuco and Southeastern ecoregion, Rio de Janeiro, São Paulo and Santa Catarina), through five polymorphic microsatellite markers. Here testing the panmixia hypothesis for Brazilian ecoregions and test natal philopathy. A total of 69 analyzed specimens revealed individual and significant genetic differentiation between the sampled locations. Φ ST (0.12), PCA, DAPC and Bayesian analyses of the genetic population structure revealed at least two distinct genetic R. bonasus groupings. IBD tests were significant, indicating a correlation between genetic and geographical distance among populations, which can be explained by reproductive philopatric behavior. Philopatric behavior associated with R. bonasus mobility may influence the differentiation values observed for all loci in the investigated samples.

show abstract

Characterisation of eleven new polymorphic microsatellite markers for the coastal stingray Dasyatis brevicaudata (Dasyatidae Hutton 1875), and cross-amplification in seven dasyatid species

Cited by 3 publications

References 17 publications

Linking local movement and molecular analysis to explore philopatry and population connectivity of the southern stingray Hypanus americanus

Linking local movement and molecular analysis to explore philopatry and population connectivity of the southern stingray Hypanus americanus

Population structure and male-biased dispersal in the short-tail stingray Bathytoshia brevicaudata (Myliobatoidei: Dasyatidae)

Genetic diversity assessment for the vulnerable migratory cownose ray Rhinoptera bonasus (Myliobatiformes: Rhinopteridae) from the southwestern Atlantic Ocean

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