Characterisation of Cultured Mesothelial Cells Derived from the Murine Adult Omentum

Dauleh, Sumaya; Santeramo, Ilaria; Fielding, Claire; Ward, Kelly; Herrmann, Anne‐Kristin; Murray, Patricia; Wilm, Bettina

doi:10.1371/journal.pone.0158997

Cited by 23 publications

(19 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Previously it was shown that mouse prMC may be cultured for more than 25-30 passages, until the symptoms of senescence become visible (22). Importantly, during this time period a subset of cells were shown to express stem cell markers such as Sox9, Sox2, CD34, Bmi1 and these cells showed adipogenic and osteogenic differentiation potential in vitro (22). This strongly indicates the presence of a prMC subpopulation with stem cell properties.…”

Section: Discussionmentioning

confidence: 95%

“…Based on the elevated Sox2/Oct4 levels necessary to drive eGFP expression, the eGFP(+) prMC might be cautiously assumed to also represent a cell population with stem cell properties. Previously it was shown that mouse prMC may be cultured for more than 25-30 passages, until the symptoms of senescence become visible (22). Importantly, during this time period a subset of cells were shown to express stem cell markers such as Sox9, Sox2, CD34, Bmi1 and these cells showed adipogenic and osteogenic differentiation potential in vitro (22).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Biological noise and positional effects influence cell stemness

Blum

Henzi

Schwaller

et al. 2018

Journal of Biological Chemistry

View full text Add to dashboard Cite

Biological (or cellular) noise is the random quantitative variability of proteins and other molecules in individual, genetically identical cells. As the result of biological noise in the levels of some transcription factors that determine a cell's differentiation status, differentiated cells may dedifferentiate to a stem cell state given a sufficiently long time period. Here, to provide direct evidence supporting this hypothesis, we used a live-cell monitoring system based on eGFP expression to continuously assess the "stemness" of individual human and murine malignant mesothelioma (MM) cells over a period of up to 3 months. Re-expression of the transcription factors, the top hierarchical stemness markers SRY-box 2 (Sox2) and octamer-binding transcription factor (Oct4), monitored as cells' eGFP expression was observed in a subpopulation of differentiated eGFP(-) MM cells. However, we found that this transition was extremely rare. Of note, when it did occur, neighboring cells that were not direct descendants of a newly emerged eGFP(+) stem cell were more likely than non-neighboring cells to also become an eGFP(+) stem cell. This observation suggested a positional effect and led to a clustered "mosaic" reappearance of eGFP(+) stem cells. Moreover, stem cells reappeared even in cell cultures derived from one single differentiated eGFP(-) cell. On the basis of our experimental in vitro and in vivo findings, we developed a tumor growth model to predict the clustered localization of cancer stem cells within a tumor mass.Regarding the heterogeneity with respect to differentiation, cancer cell populations consist of cancer stem cells (CSC) and a majority of non-CSC or 'bulk' cancer cells. It is hypothesized that essentially the CSC subpopulation possesses the capability of tumor initiation and has the capacity for self-renewal (1,2). Two main theories were initially developed to explain the distribution of CSC within a tumor: the hierarchical and the stochastic model (1). The hierarchical model suggests that the pool of CSC can only be maintained by cells that have CSC characteristics and by definition, the ability to give rise to progeny with unlimited proliferative capacity. Thus, tumors contain: I) proliferating CSC that The cells in such a tumor do not operate in a deterministic, "well-organized" system -any cell has the same intrinsic potential to contribute to tumor growth. Unlike in the hierarchical model, the stochastic model predicts that CSC are not necessarily and exclusively derived from the CSC cell population. Currently, there is no definitive proof in favor of either model of tumor growth. The development of different cancer types may be explained in different ways; leukemia are thought to mostly follow the hierarchical model (3), while breast cancers likely develop according to the stochastic approach (4).Malignant mesotheliomas (MM) are tumors originating from the serosal cells covering the pleural, peritoneal or pericardial cavities. MM are highly aggressive neoplasms most often associated with...

show abstract

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Biological noise and positional effects influence cell stemness

Blum

Henzi

Schwaller

et al. 2018

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…Furthermore, it is not clear whether postnatal or even adult mesothelial cells in the peritoneal cavity generally contribute to the maintenance of the intestinal and parietal body wall. This is pertinent because of reports suggesting that mesothelial cells have the plasticity to differentiate into cells of different mesodermal lineages (Colunga et al, 2019, Karki et al, 2014, Mutsaers et al, 2015, Dauleh et al, 2016.…”

Section: Introductionmentioning

confidence: 99%

Restricted differentiative capacity of Wt1-expressing peritoneal mesothelium in postnatal and adult mice

Wilm

Tanton

Mutter

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

The mesoderm gives rise to diverse tissues including the mesothelium, as well as the visceral and vascular smooth muscle. Previously, genetic lineage tracing based on the mesothelial marker Wt1, appeared to show that visceral mesothelial cells are the direct progenitors of vascular smooth muscle in the intestine. However, the timing and underlying developmental mechanisms regulating this lineage were not fully understood. Here, using a temporally-controlled Wt1-based genetic lineage tracing approach, we demonstrate that (i) the adult visceral mesothelium of the intestine only maintains itself and fails to contribute to other visceral tissues; (ii) the vascular and visceral smooth muscle in the developing intestine and mesentery arise earlier than and independently from the visceral mesothelium. Our results suggest that Wt1 is switched on and remains expressed in the visceral mesothelium from around E9.5 onwards throughout life, while earlier transient expression of Wt1 in the nascent mesoderm specifies the future vascular and visceral smooth muscle of intestine and mesentery.

show abstract

“…La baja expresión de marcadores HLA tipo I y II y la ausencia de algunas moléculas co-estimuladoras como CD80, CD86 y CD40 ha favorecido su uso alogénico en diferentes patologías por su baja inmunogenicidad. Sin embargo, existe cierta controversia en la literatura sobre si el proceso de diferenciación a otras estirpes celulares favorece la expresión de marcadores HLA (15)(16)(17)(18)(19)(20).…”

Section: Introductionunclassified