Characterisation of choline esterases and their tissue and subcellular distribution in mussel (Mytilus edulis)

Brown, Margaret; Davies, Ian; Moffat, Colin F.; Redshaw, John; Craft, John A.

doi:10.1016/s0141-1136(03)00067-9

Cited by 47 publications

(19 citation statements)

References 21 publications

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“…The decrease in AChE activity in the oyster was efficiently used as a biomarker of exposure to neurotoxic compounds. Moreover, the kinetic and toxicological properties of AChE in bivalves have been extensively studied, allowing the optimization of the assays to measure AChE activity in different species, [24]. …”

Section: Resultsmentioning

confidence: 99%

Reduced glutathione and acetylcholinesterase expressions in Perna indica exposed to trivalent arsenic

Rajkumar¹

2013

International Journal of Biological Research

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In the present study Indian brown mussel, Perna indica was exposed to 20, 40, 80, 160 and 320 ppb (µg/l) arsenic concentrations under short-term chronic toxicity bioassay (14 days) for the assessment of reduced glutathione and acetylcholinesterase as potential biochemical biomarkers in arsenic pollution. A higher level of reduced glutathione (GSH) and increased acetylcholinesterase (AchE) activity was expressed when compared to control in 160 and 320 ppb arsenic concentration. The found values were significant at P<0.01. The values of total protein were significantly lower (P<0.05) in all the exposed concentration when compared with control. This study suggests that metabolites and antioxidant enzymes activity was affected by heavy metal exposures and strongly have the potential as indicators of heavy metal contamination and arsenic in specific. Hence, the determination of oxidative stress biomarkers in Perna indica may serve as a convenient approach during pollution biomonitoring programme.

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Section: Resultsmentioning

confidence: 99%

Reduced glutathione and acetylcholinesterase expressions in Perna indica exposed to trivalent arsenic

Rajkumar¹

2013

International Journal of Biological Research

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“…The homogenate was centrifuged (Beckman, Model J-21C centrifuge) for 20 min at 10,000 g and at 48C. After centrifugation, the supernatant was analyzed for enzyme activity spectrophotometrically at 540 nm, using a UV-VIS microplate reader (SpectraFluor Plus), following the method described by Ellman et al [19], and adapted for the analysis of mussels by Brown et al [20]. Results of AChE activity analysis were expressed as mU/ganglion.…”

Section: Biochemical Analysesmentioning

confidence: 99%

Physiological effects of temperature and a herbicide mixture on the soft‐shell clam Mya arenaria (Mollusca, Bivalvia)

Greco

Pellerin

Capri

et al. 2010

Enviro Toxic and Chemistry

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Abstract-The aim of the current study was to investigate effects of temperature and a mixture of herbicides on the physiological status of the bivalve Mya arenaria. Bivalves acclimated to two temperatures (7 and 188C) were exposed for 28 d to 0.01 mg/L of a pesticide formulation containing dichlorophenoxyacetic acid (2,4-D), 2-(2-methyl-4-chlorophenoxy) propionic acid (mecoprop), and 3,6-dichloro-2-methoxybenzoic acid (dicamba). At days 7, 14, and 28, mortality, immune parameters (hemocyte number, phagocytic activity, and efficiency), biomarkers of oxidative stress (catalase [CAT] and superoxide dismutase [SOD] activities and malondialdehyde [MDA] content), the metabolic enzyme cytochrome C oxidase (CCO), a biomarker of pesticide exposure (acetylcholinesterase [AChE]), and the activity of an enzyme related to gametogenesis (aspartate transcarbamylase [ATCase]) were monitored in clam tissues. Gonadosomatic index (GSI), condition factor (CF), and sex were also assessed. In clams acclimated to 78C, exposure to pesticide enhanced CCO activity and CF and decreased MDA content, hemocyte number, CAT, and SOD activities. In clams kept at 188C, pesticide effects appeared minor compared with samples kept at 78C. In bivalves acclimated to 188C, CCO, SOD, and ATCase activity and MDA content were enhanced, and hemocyte number, CAT, and AchE activities and phagocytosis were suppressed. In samples exposed to pesticides, increased temperature enhanced MDA content and CCO and SOD activity and suppressed hemocyte number and CAT and AchE activity. A gradual sexual maturation was observed in both sexes through experimental time, but females had a higher sensitivity to temperature and pesticides compared to males. Increased temperature altered the ability of the sentinel species Mya arenaria to respond to pesticide exposures. Further work is needed to understand the impacts of increasing temperature on the whole St. Lawrence estuary ecosystem. Environ. Toxicol. Chem. 2011;30:132-141. # 2010 SETAC

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“…The sensitivity to OP of gills has been observed in previous works on both marine and freshwater bivalves (Escartin and Porte 1997;McHenery et al 1997;Mora et al 1999;Lehtonen and Leinio 2003;Bonacci et al 2004;Brown et al 2004). This property has been explained as an aspect of the role of the gills as a first line of defense against contaminants and pollutants.…”

Section: Discussionmentioning

confidence: 56%

Acetylcholinesterase activity in Corbicula fluminea Mull., as a biomarker of organophosphate pesticide pollution in Pinacanauan River, Philippines

Beltran

Pocsidio

2009

Environ Monit Assess

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Organophosphates are known to inhibit the enzyme acetylcholinesterase. In this study, the AChE activity from the total soft tissues of Corbicula fluminea Mull. was used as a biomarker of organophosphate pollution in Pinacanauan River. Clams were collected from two different sites and at different seasons of the year. A colorimetric assay on the total soft tissues of the clams showed a directly proportional relationship between enzyme activity and condition of the riverine system. In vitro experiments on the total soft tissue, adductor muscles, digestive glands, and gills were conducted to assess the degree of localization of AChE as well as the sensitivity and tolerance of the enzymes in these tissues to varying concentrations of malathion. The degree of enzyme localization from highest to lowest is as follows: adductor muscle > gills > digestive gland whereas sensitivity to OP from greatest to least is: gills > adductor muscles > digestive gland.

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Characterisation of choline esterases and their tissue and subcellular distribution in mussel (Mytilus edulis)

Cited by 47 publications

References 21 publications

Reduced glutathione and acetylcholinesterase expressions in Perna indica exposed to trivalent arsenic

Reduced glutathione and acetylcholinesterase expressions in Perna indica exposed to trivalent arsenic

Physiological effects of temperature and a herbicide mixture on the soft‐shell clam Mya arenaria (Mollusca, Bivalvia)

Acetylcholinesterase activity in Corbicula fluminea Mull., as a biomarker of organophosphate pesticide pollution in Pinacanauan River, Philippines

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