Characterisation of cell wall polysaccharides from rapeseed (Brassica napus) meal

Improving the nutritional value of rapeseed cake and wheat dried distillers grains with solubles by addition of enzymes during liquid fermentation

“…This indicated the presence of hemicellulose in the form of xyloglucans and xylans, and pectin, which were also found in other studies analysing the polysaccharide fraction of RSC and RSM (Ghosh et al, 2004;Pustjens et al, 2013). Rapeseed co-products are characterised by high levels of pectin (Egües et al, 2010;Pustjens et al, 2013); this was confirmed in the current study by the presence of 58 g uronic acids/kg DM. Pectic structures often have arabinan side-chains (Thakur et al, 1997), which are responsible for the high level of arabinose in the samples (48 g/kg).…”

“…Pectic structures often have arabinan side-chains (Thakur et al, 1997), which are responsible for the high level of arabinose in the samples (48 g/kg). Other possible sources of arabinose in rapeseed co-products are arabinoxylans, arabinans and arabinogalactans (Ghosh et al, 2004;Egües et al, 2010;Pustjens et al, 2013). Due to the high amount of pectin in RSC and RSM, the cell wall is very tightly associated (Pustjens et al, 2013), which together with the relatively high lignin content of RSC (Gdala et al, 1997), are responsible for the high insolubility of NSP in the RSC (in the current study 83% of total NSP being insoluble).…”

“…Other possible sources of arabinose in rapeseed co-products are arabinoxylans, arabinans and arabinogalactans (Ghosh et al, 2004;Egües et al, 2010;Pustjens et al, 2013). Due to the high amount of pectin in RSC and RSM, the cell wall is very tightly associated (Pustjens et al, 2013), which together with the relatively high lignin content of RSC (Gdala et al, 1997), are responsible for the high insolubility of NSP in the RSC (in the current study 83% of total NSP being insoluble). Lignin is a phenolic compound which cross-link to cell wall polysaccharides and hereby cements the cell wall (Iiyama et al, 1994).…”

“…Of the enzyme treatments GluXylPec (Enz. 6) was the only treatment showing a decrease in total NSP and the highest decrease of insoluble NSP, possibly because of the pectinase activity that solubilises pectins from the hull fraction of RSC/RSM (Pustjens et al, 2013). The method used in the present study to determine NSP determines polymers with a degree of polymerization of minimum ten.…”

Changes in chemical composition of rapeseed meal during storage, influencing nutritional value of its protein and lipid fractions

“…However, the dietary inclusion of meal, in particular for monogastric animals, is limited due to high content of polysaccharides (crude fibre) (Pustjens et al, 2013) and non-nutritive substances -glucosinolates (Bell, 1995;Tripathi and Mishra, 2007), polyphenolic compounds (Naczk et al, 1998) and phytates (Thompson and Serraino, 1986). The content and digestibility of amino acids is considered to be the main factor influencing effective animal nutrition.…”