Characterisation of Carotenoids Involved in the Xanthophyll Cycle

Kuczyńska, Paulina; Jemioła-Rzemińska, Małgorzata; KazimierzStrzalka,

doi:10.5772/67786

Cited by 7 publications

(7 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…VDL1 catalyzes the conversion of violaxanthin to neoxanthin, which serves as a pool of precursors for FX synthesis. Moreover, VDL1 was previously shown to be induced by HL stress, suggesting its crucial role in the xanthophyll cycle in P. tricornutum (Kuczynska et al 2017). In this study, PtCRTISO isoforms 1, 2, and 4 were upregulated in the LL culture on day 5; however, no previous findings demonstrate a positive correlation between CRTISO expression and FX biosynthesis.…”

Section: Discussioncontrasting

confidence: 66%

Interdependence of fucoxanthin biosynthesis and fucoxanthin-chlorophyll a/c binding proteins in Phaeodactylum tricornutum under different light intensities

Truong

Park²,

Koo³

et al. 2022

J Appl Phycol

View full text Add to dashboard Cite

Fucoxanthin (FX), a primary carotenoid, is associated with the fucoxanthin-chlorophyll a/c binding protein (FCP) complex integrated into the thylakoid membrane (TM) which functions as a light-harvesting complex in the diatom Phaeodactylum tricornutum. Here, we aimed to elucidate the FX production regulated by different light intensities via the correlation of FX biosynthesis and apoproteins composing of FCP complex. High light (HL) accelerated P. tricornutum growth more than low light (LL). The maximum values of FX content and productivity obtained under LL (1.7 mg g−1 and 2.12 mg L−1 day−1, respectively) were substantially higher than those obtained under HL (0.54 mg g−1 and 0.79 mg L−1 day−1, respectively). Notably, proteome and photosynthetic pigment analyses revealed the enrichment of FCP antennae in the LL culture TM fractions but not the HL culture. Semi-quantification of FCP antenna protein using LC–MS/MS and RNA transcriptome analyses revealed that PtLhcf5 and PtLhcf8 played crucial roles in FCP biosynthesis under LL. P. tricornutum cultured under light transition exhibited FCP formation only in the early growth stage to meet the increased photosynthetic activity requirements under LL. Meanwhile, FCP degradation could be triggered by HL throughout the cultivation period. Therefore, FX production was highly correlated with FCP formation, and LL conditions in the early growth stage were critical for higher FX productivity.

show abstract

Section: Discussioncontrasting

confidence: 66%

Interdependence of fucoxanthin biosynthesis and fucoxanthin-chlorophyll a/c binding proteins in Phaeodactylum tricornutum under different light intensities

Truong

Park²,

Koo³

et al. 2022

J Appl Phycol

View full text Add to dashboard Cite

show abstract

“…FCPs belong to the superfamily of transmembrane LHC proteins currently well-described in diatoms (Büchel, 2020b). FCPs bind photosynthetic pigments such as Fx, chlorophylls a and c (Chl a/c), and diadinoxanthin (Ddx), which can be de-epoxidized in diatoxanthin (Dtx) for photoprotection purposes (Lavaud et al, 2002(Lavaud et al, , 2004Kuczynska et al, 2015). In particular, the spatial organization of FCPs and their carotenoids confer to Chromista a very strong absorption capacity in the blue-green spectral range of visible light, which are the most available radiations in the water column (Wang et al, 2019a,b).…”

Section: Introductionmentioning

confidence: 99%

The Fucoxanthin Chlorophyll a/c-Binding Protein in Tisochrysis lutea: Influence of Nitrogen and Light on Fucoxanthin and Chlorophyll a/c-Binding Protein Gene Expression and Fucoxanthin Synthesis

et al. 2022

View full text Add to dashboard Cite

We observed differences in lhc classification in Chromista. We proposed a classification of the lhcf family with two groups specific to haptophytes, one specific to diatoms, and one specific to seaweeds. Identification and characterization of the Fucoxanthin and Chlorophyll a/c-binding Protein (FCP) of the haptophyte microalgae Tisochrysis lutea were performed by similarity analysis. The FCP family contains 52 lhc genes in T. lutea. FCP pigment binding site candidates were characterized on Lhcf protein monomers of T. lutea, which possesses at least nine chlorophylls and five fucoxanthin molecules, on average, per monomer. The expression of T. lutea lhc genes was assessed during turbidostat and chemostat experiments, one with constant light (CL) and changing nitrogen phases, the second with a 12 h:12 h sinusoidal photoperiod and changing nitrogen phases. RNA-seq analysis revealed a dynamic decrease in the expression of lhc genes with nitrogen depletion. We observed that T. lutea lhcx2 was only expressed at night, suggesting that its role is to protect \cells from return of light after prolonged darkness exposure.

show abstract

“…tricornutum 43 . In this study, PtCRTISO isoforms 1, 2, and 4 were upregulated in the LL culture on day 5; however, no previous ndings demonstrate a positive correlation between CRTISO expression and FX biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Mechanism of fucoxanthin biosynthesis in Phaeodactylum tricornutum under different light intensities via FCP complex formation

Truong

Park²,

Koo³

et al. 2022

Preprint

View full text Add to dashboard Cite

Fucoxanthin (FX), a primary carotenoid, is associated with the fucoxanthin-chlorophyll a/c binding protein (FCP) complex integrated into the thylakoid membrane (TM) which functions as a light-harvesting complex in Phaeodactylum tricornutum. Here, we aimed to elucidate the mechanism of FX biosynthesis under different light intensities via FCP complex formation. High light (HL) accelerated P. tricornutum growth more than low light (LL). The maximum values of FX content and productivity obtained under LL (1.7 mg/g and 2.12 mg/L/day, respectively) were substantially higher than those obtained under HL (0.54 mg/g and 0.79 mg/L/day, respectively). Notably, SDS-PAGE and photosynthetic pigment analyses revealed the enrichment of FCP antennae in the LL culture TM fractions but not the HL culture. Semi-quantification of FCP antenna protein using LC-MS/MS and RNA transcriptome analyses revealed that PtLhcf5 and PtLhcf8 played crucial roles in FCP biosynthesis under LL. P. tricornutum cultured under light transition exhibited FCP formation only in the early growth stage to meet the increased photosynthetic activity requirements under LL. Meanwhile, FCP degradation could be triggered by HL throughout the cultivation period. Therefore, FX production was highly correlated with FCP formation, and LL conditions in the early growth stage were critical for higher FX productivity.

show abstract

Characterisation of Carotenoids Involved in the Xanthophyll Cycle

Cited by 7 publications

References 49 publications

Interdependence of fucoxanthin biosynthesis and fucoxanthin-chlorophyll a/c binding proteins in Phaeodactylum tricornutum under different light intensities

Interdependence of fucoxanthin biosynthesis and fucoxanthin-chlorophyll a/c binding proteins in Phaeodactylum tricornutum under different light intensities

The Fucoxanthin Chlorophyll a/c-Binding Protein in Tisochrysis lutea: Influence of Nitrogen and Light on Fucoxanthin and Chlorophyll a/c-Binding Protein Gene Expression and Fucoxanthin Synthesis

Mechanism of fucoxanthin biosynthesis in Phaeodactylum tricornutum under different light intensities via FCP complex formation

Contact Info

Product

Resources

About