Characterisation of basal resistance (BR) by expression patterns of newly isolated representative genes in tobacco

Szatmári, Ágnes; Ott, Péter G.; Varga, G; Besenyei, E.; Czelleng, A.; Klement, Z.; Bozsó, Zoltán

doi:10.1007/s00299-005-0110-5

Cited by 23 publications

(25 citation statements)

References 70 publications

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“…Photoassimilate-responsive (PAR)-like genes (TC77234 and TC77235) are examples of the crosstalk between sugar metabolism and defense responses against pathogens that were up-regulated in bacterially infiltrated samples (Herbers et al 1995;Takemoto et al 2003;Szatmari et al 2006). Genes that are involved in ethylene and polyamine synthesis (S-adenosyl-L-methionine synthetases) were also activated both in P. syringae hrcC-and P. syringae-treated leaves at 6 hpi.…”

Section: Defense-associated Genesmentioning

confidence: 92%

Transcriptome analysis of a bacterially induced basal and hypersensitive response of Medicago truncatula

Bozsó

Maunoury²,

Szatmári³

et al. 2009

Plant Mol Biol

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Research using the well-studied model legume Medicago truncatula has largely focused on rhizobium symbiosis, while little information is currently available for this species on pathogen-induced transcriptome changes. We have performed a transcriptome analysis of this species with the objective of studying the basal (BR, no visible symptoms) and hypersensitive response (HR, plant cell death) in its leaves at 6 and at 24 h after infection by HR-negative (hrcC mutant) and HR-inducing Pseudomonas syringae pv. syringae strains, respectively. Although there were no visible symptoms at the BR, the alterations in gene expression were comparable to those found with the HR. Both responses resulted in the transcriptional alteration of hundreds of plant genes; however, the responses in the HR were usually more intense. The reactions to HR-inducing and HR-negative bacterial strains were significantly overlapping. Parallel up- or down-regulation of genes with the same function occurred frequently. However, some plant processes were regulated in one direction; for example, most of the protein synthesis-related genes were activated and all of the photosynthetic/chloroplast genes were suppressed during BR. The possible roles of several functional classes (e.g., cell rescue, signaling, defense, cell death, etc.) of transcriptionally altered genes are discussed. The results of the comparison with available mycorrhizal and nodule expression data show that there is a significant overlap between nodulation and the leaf defense response and that during the early stage of the nodulation in roots, Sinorhizobium meliloti induces a fluctuation in the transcription of BR- and HR-responsive genes.

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Section: Defense-associated Genesmentioning

confidence: 92%

Transcriptome analysis of a bacterially induced basal and hypersensitive response of Medicago truncatula

Bozsó

Maunoury²,

Szatmári³

et al. 2009

Plant Mol Biol

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“…The defense is defined as basal defense and is reportedly induced by hrp-deficient bacterial pathogens (Klement et al, 1999;Szatmari et al, 2005). Basal defense is also activated by virulent pathogens on susceptible hosts (Jones and Dangl, 2006).…”

Section: Discussionmentioning

confidence: 99%

S-Glycoprotein-Like Protein Regulates Defense Responses in Nicotiana Plants against Ralstonia solanacearum

et al. 2010

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RsRGA4 (for Ralstonia solanacearum-responsive gene A4) encodes a polypeptide similar to S-locus glycoprotein (SGP) from Brassica rapa and SGP-like proteins from Ipomoea trifida and Medicago truncatula. Therefore, we designated RsRGA4 as NtSGLP (for Nicotiana tabacum SGP-like protein) and NbSGLP (its Nicotiana benthamiana ortholog). NbSGLP is expressed in root, leaf, petal, gynoecium, and stamen. Expression of NbSGLP was strongly induced by inoculation with an avirulent strain of R. solanacearum (Rs8107) and slightly enhanced by inoculation with virulent R. solanacearum (RsOE1-1). Expression of NbSGLP was induced by inoculation with an hrpY-deficient mutant of RsOE1-1 and Rs8107. Expression was also induced by aminocyclopropane carboxylic acid and salicylic acid. Virus-induced gene silencing of NbSGLP enhanced the growth of Rs8107. Growth of RsOE1-1 and appearance of wilt symptoms were also accelerated in silenced plants. Expression of PR-1a and EREBP was reduced, and markers for basal defense, such as callose deposition and reduced vascular flow, were compromised in NbSGLP-silenced plants. Moreover, growth of Pseudomonas cichorii, Pseudomonas syringae pv tabaci, and P. syringae pv mellea was also enhanced in the silenced plants. On the other hand, silencing of NbSGLP did not interfere with the appearance of the hypersensitive response. NbSGLP was secreted in a signal peptide-dependent manner. Agrobacterium tumefaciens-mediated expression of NbSGLP induced PR-1a and EREBP expression, callose deposition, and reduced vascular flow. NbSGLP-induced callose deposition and reduced vascular flow were not observed in salicylic acid-deficient N. benthamiana NahG plants. Taken together, SGLP might have a role in the induction of basal defense in Nicotiana plants.

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“…Effector proteins are recognized by plant cells after which the HR-based defense develops. Lack of type III secretion apparatus (the hrp mutant) results in loss of pathogenicity and the ability to induce a HR (Kanda et al, 2003a;Szatmari et al, 2005). However, hrp mutant bacteria still induce basal resistance (Klement et al, 1999;Szatmari et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…Lack of type III secretion apparatus (the hrp mutant) results in loss of pathogenicity and the ability to induce a HR (Kanda et al, 2003a;Szatmari et al, 2005). However, hrp mutant bacteria still induce basal resistance (Klement et al, 1999;Szatmari et al, 2005). Basal resistance is triggered by recognition of general conserved elicitors, so-called pathogen-associated molecular patterns, and defends all plant species against most microbes (Nurnberger and Brunner, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…Relative quantification of gene expression was carried out according to the instructions for the Applied Biosystems 7300 real-time PCR system, using the comparative cycle threshold method for the calculation of Qty value. All values were normalized to the expression values of the actin gene as an internal standard in each cDNA stock, as described previously (Szatmari et al, 2005), and were shown relative to the absolute 0 time control level. Expression analyses were carried out with at least two biological replications to ensure that expression patterns were reproducible.…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

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Induction of a Small Heat Shock Protein and Its Functional Roles inNicotianaPlants in the Defense Response againstRalstonia solanacearum

Maimbo

Ohnishi²,

Hikichi³

et al. 2007

Plant Physiology

111

117

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In tobacco (Nicotiana tabacum), Ralstonia solanacearum OE1-1 (RsOE1-1) is pathogenic, whereas R. solanacearum 8107 (Rs8107) is nonpathogenic and induces the hypersensitive response (HR). To elucidate the molecular mechanisms of plant-R. solanacearum interactions, we used differential display to isolate a cDNA fragment, A6, regulated in tobacco by inoculation with RsOE1-1. The deduced amino acid sequence predicted from full-length A6-cDNA showed similarity to small heat shock proteins from Arabidopsis (Arabidopsis thaliana; hypothetical protein), Medicago truncatula, and Cucumis melo; we therefore designated A6 to correspond to Ntshsp17 (for tobacco small heat shock protein 17). Recombinant Ntshsp17 overproduced in Escherichia coli exhibited molecular chaperone function. Expression of Ntshsp17 was increased in tobacco leaves inoculated with both RsOE1-1 and Rs8107. Expression was induced by heat treatment and by treatment with aminocyclopropane carboxylic acid, hydrogen peroxide, methyl jasmonate, and salicylic acid. Ntshsp17 expression was induced by inoculation with a HR and pathogenicity gene mutant of Rs8107 that does not induce the HR, but not by Agrobacterium-mediated transient expression of INF1, an HR elicitor. In Nbshsp17-silenced plants (an Ntshsp17 ortholog in Nicotiana benthamiana), expression of ETHYLENE-RESPONSE ELEMENT-BINDING PROTEIN, PATHOGENESIS-RELATED1a (PR1a), and PR4 genes was compromised, but expression of ELONGATION FACTOR1a was scarcely affected. Appearance of the HR was not affected in the silenced plants. In the silenced plants, growth of Rs8107 was accelerated. Bacterial growth and wilt symptoms elicited by RsOE1-1 were also accelerated in the silenced plants. These results indicate that this small heat shock protein might have a role in HR-independent defenses in Nicotiana plants.

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Characterisation of basal resistance (BR) by expression patterns of newly isolated representative genes in tobacco

Cited by 23 publications

References 70 publications

Transcriptome analysis of a bacterially induced basal and hypersensitive response of Medicago truncatula

Transcriptome analysis of a bacterially induced basal and hypersensitive response of Medicago truncatula

S-Glycoprotein-Like Protein Regulates Defense Responses in Nicotiana Plants against Ralstonia solanacearum

Induction of a Small Heat Shock Protein and Its Functional Roles inNicotianaPlants in the Defense Response againstRalstonia solanacearum

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