Characterisation of bare and tannase-loaded calcium alginate beads by microscopic, thermogravimetric, FTIR and XRD analyses

Abstract

Incorporating enzymes into calcium alginate beads is an effective method to immobilise them and to preserve, at the same time, their catalytic activity. Sodium alginate was mixed with Aspergillus ficuum tannase in aqueous solution, and tannase-loaded calcium alginate beads were prepared using a simple droplet-based microfluidic system. Extensive experimental analysis was carried out to characterise the samples. Microscopic imaging revealed morphological differences between the surfaces of bare alginate matrix and tannase-loaded alginate beads. Thermal analysis allowed assessing the hydration contents of alginate and revealed the presence of tannase entrapped in the loaded beads, which was confirmed by vibrational spectroscopy. X-ray diffraction allowed us to conclude that alginate of tannase-loaded beads is not crystalline, which would make them suitable as carriers for possible controlled release. Moreover, they could be used in food applications to improve tea quality or clarify juices.

Antimicrobial functionalization of Ca alginate‐coconut oil latent heat storing microcapsules by Ag nanoparticles

“…The mass loss of 30.2% up to 185 C accompanied by an endotherm is due to the release of physically bound and most probably structural water too. 43 The chemical degradation of the polysaccharide rings takes place in the next two mass loss steps (185 C-258 C Δm −9.2% and 258 C-368 C Δm −10.9%), while the oxidative burning of the remaining organic material takes place in another two-step process, between 368 C and 557 C (Δm −10.6%) and 557 C and 668 C (Δm −2.5%). This oxidative degradation/burning step is also confirmed by series of overlapped exothermic peaks.…”

Fabrication of detonation nanodiamond@sodium alginate hydrogel beads and their performance in sunlight-triggered water release

“…Characteristic peaks for calcium alginate are known to appear at angles of 13 , 56 , 20.64 , 20.06 , 28.96 , and 36.40 , thus demonstrating its semi-crystalline nature. 48 The DND@SA composites showed two broad peaks at 2q of 22 , and 39 as observed in Fig. 2b.…”

“…The TGA proles of both types of beads can be analyzed within three steps: the rst step occurring in the range of 25-200 C, the second occurring in the range of 200-400 C, and the last occurring in the range of 400-700 C. The rst step accounts for about 15% of weight loss, which can be attributed to the desorption of physically adsorbed water and removal of structural water, thus indicating excellent water adsorption characteristics of both types of beads. 48,52 The second step, which is the rapid weight loss stage, corresponds to the thermal cracking processes of some functional groups (such as -OH and -COOH, etc.) and cation bonding bridges, the volatilization of small molecular substances, and the formation of carbonized products, while the bottom to the further decomposition of carbonized products and the removal of residual volatile components.…”

Development and Characterization of Colon-targeting 5-Fluorouracil Multiparticulate Beads

“…The spectroscopic data was recorded over a wave number region of 4000 to 400 cm -1 . FTIR spectra of 5-FU, SA, FSM, and a physical mixture of drug and polymers (1:1:1) and the developed FSM-SA beads were recorded at the Indian Institute of Technology, Roorkee, Uttarakhand, India [36] .…”