Characterisation of an Acrylamide-Degrading Bacterium and Its Degradation Pathway

Abstract

Widespread use of acrylamide by the industry led to the environmental pollution that results from the indiscriminate discharge of acrylamide. The presence of acrylamide in the environment is a major threat due to its neurotoxic, carcinogenic and teratogenic properties. In this work bacterial isolate identified as Burkholderia sp. strain AQ5-13 is capable of degrading acrylamide as both its carbon and nitrogen sources were screen based on the capability of the bacteria to grow on solid basal salt media that contain 500 mg/L acrylamide as sole carbon and nitrogen sources without supplementation of additional carbon or nitrogen sources. Bacteria grow curve study was carried out by measuring the absorbance value at 600 nm. Burkholderia sp. strain AQ5-13 showed a complete life cycle in five days of incubation with no lag phase identified at the earlier growth phase. Optimum conditions for acrylamide degradation was conducted at different initial pH and incubation temperature. Growth optimisation of the bacteria was measured by plate counting in CFU after 48 h of incubation in 250 mL basal salt media that contain 500 mg/L acrylamide. Burkholderia sp. strain AQ5-13 showed optimum growth in acidic media at pH 5.7 (5.57 log 10 CFU/mL) and optimum growth at 30°C (6.84 log 10 CFU/mL) compared to other temperature ranges. Quantitative monitoring of acrylamide degradation was performed using HPLC. Burkholderia sp. strain AQ5-13 could degrade 14% of 500 mg/L acrylamide as its carbon and nitrogen sources after 72 h of incubation at 5 min of retention time. The appearance of acrylic acid peak as an intermediate was not detected, possibly because all the acrylic acid produced had been consumed immediately by the bacterium. The result from this study showed that bacteria Burkholderia sp. strain AQ5-13 has a good potential that can be applied in the bioremediation of waste containing acrylamide.

Characterizing the Potentials of Alcaligenes sp. for Acrylamide Degradation

“…The creation of acrylamide, which is carcinogenic and neurotoxic [4,7], can occur during the cooking process known as the Maillard reaction. Due to its neurotoxic, carcinogenic, and teratogenic qualities [5,8] mentioned previously, acrylamide poses a significant environmental risk [8].…”

“…Polyacrylamide, which is made from acrylamide, is used to purify water as well as in the printing, plastics, and adhesives industries [3,4]. As a result of its widespread usage in industry, acrylamide has been linked to environmental contamination caused by its careless disposal [5]. Carbohydrate-rich meals that are cooked at high temperatures through a mechanism called the Maillard reaction [6] produce acrylamide, which is among the neurotoxic and carcinogenic chemicals.…”

“…Since acrylamide monomer was used by practically every industry. It's crucial that acrylamide and its derivatives be removed from industrial effluents before they're released into the environment [5]. Therefore, it is necessary to address this issue by concentrating on biological acrylamide remediation [6].…”

Inhibition Kinetics Study of Molybdenum Reduction by Pantoea sp. strain HMY-P4

“…Molybdenum is an emerging pollutants of global concern [1]. Microorganisms are theoretically used quicker, easier and safer for the removal of molybdenum than traditional and physical approaches especially in cases of soil pollution where these other methods will be less effective or highly costly [2]. Molybdenum remediation on areas polluted by molybdenum was effective in mitigating toxic effects of molybdenum using a bacteria consortium on bovine pasture lands in Tyrol, Austria [3].…”

Modelling the Inhibition Kinetics of Molybdenum Reduction by the Molybdate-reducing Enterobacter cloacae

“…Microorganisms potentially detoxify molybdenum faster, easier and safer than conventional and physical approaches especially when soil contamination is the main target. Under this condition other methods are likely less efficient or expensive [2]. Molybdenum can be presented in the form of molybdenite, a byproduct of gold and copper mining.…”