Characterisation of Aerobic Bacteriological Isolates From Orthopedic Implant Site Infections With Special Reference to Biofilm Formation in a Teritiary Care Hospital

S, Roopa Shree; Prathab, A G

doi:10.14260/jemds/2015/825

Cited by 3 publications

(3 citation statements)

References 10 publications

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“…In the present study, majority of patients were from 31-40-year age group, which is in concordance with other studies [15,18] , this may be due to high activity of the individuals for their daily routine work and have high risk to sustain injury and road traffic accidents.…”

Section: Discussionsupporting

confidence: 93%

Detection of Biofilm Production and Antibiotic Resistance Pattern in Clinical Isolates from Orthopaedic Implants Associated Infections

Shakthi,

Venkatesha

2023

JMSH

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Introduction: Orthopaedic Implant associated infection is the major problem which leads to implant failure and economic burden to the patient. Implant-associated infections are due to the formation of biofilm at the implantation site leading to multidrug resistant organism. Aim: This study was done to evaluate the causative organisms, their antibiotic sensitivity pattern and their ability to form biofilm over the Implants used in Orthopaedic surgeries. Material and methods: This study was carried out in the department of Microbiology, Adichunchanagiri institute of Medical sciences from September 2019 to August 2022. Swabs from 100 patients who had undergone orthopaedic implant or prosthetic surgery and presented with signs and symptoms of infection were collected and processed as per standard procedures. All the isolates were subjected to detect biofilm production by Congo red agar method, MRSA detection by Cefoxitin disc diffusion test, vancomycin resistant Enterococcus by using Vancomycin (Minimum inhibitory concentration) MIC strips and Extended spectrum β lactamases (ESBL) production by phenotypic confirmatory combined-disc test. Results: Out of the 100 samples processed, culture positivity was observed in 72 specimens. Among them, Staphylococcus aureus 19(26%) was the predominant isolate and 44 (61%) are biofilm producers. 17 (89%) were MRSA (Methicillin resistance Staphylococcus aureus) strains and 15(37%) were ESBL producing strains. Conclusion: The appropriate pre- and post- operative care should be taken to prevent orthopaedic implant associated infections. Staphylococcus spp was the commonest isolate and its ability to produce biofilm indicates the need for an appropriate antibiotic policy and screening for MRSA carriers to reduce the infection. Keywords: Orthopaedic implant, Infection, Biofilm, MRSA, ESBL

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Section: Discussionsupporting

confidence: 93%

Detection of Biofilm Production and Antibiotic Resistance Pattern in Clinical Isolates from Orthopaedic Implants Associated Infections

Shakthi,

Venkatesha

2023

JMSH

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“…24 Roopashree et al studied orthopedic SSIs and reported 72% isolates as biofilm producers. 25 All the studies reported biofilm production rate to be significantly high.…”

Section: Discussionmentioning

confidence: 99%

“…Vancomycin and linezolid were found effective against all the isolates. 25 Other researchers also studied susceptibility pattern of Gram-positive organisms and reported majority isolates showed resistance against penicillin, cephalexin, erythromycin, clindamycin, amoxycillinclavulanate, cefoxitin and cotrimoxazole. No resistance was observed towards vancomycin and linezolid.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of bacterial isolates causing surgical site infection with an emphasis on their biofilm forming capacity

Asati,

Kumar

2024

IJLBPR

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Bacterial Aetiological Agents in Infected Orthopaedic Implants - A Cross Sectional Study from Andhra Medical College, Visakhapatnam

Cheemala¹,

Koripella²,

Perala³

2021

jebmh

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BACKGROUND Orthopaedic implant site infection is one of the major problems of surgical site infection associated with high morbidity and mortality. As implants are commonly used in orthopaedic procedures and especially when preceded by trauma, orthopaedic procedures are more prone for surgical site infections. We wanted to study the aerobic bacterial aetiology of infected orthopaedic implants. METHODS This was a cross sectional study carried out over a period of one and half years. The study group comprised of 100 patients who had undergone orthopaedic prosthetic implant surgeries and presented with signs and symptoms of infections. The demographic data were recorded, type of surgery, the time of infection during postoperative period and risk factors were noted. Serous / purulent discharge adjacent to infected implants were processed in the laboratory as per the standard protocol. RESULTS Among the 100 samples studied, 79 % were culture positive and 21 % culture sterile. 55.7 % were Gram-positive cocci isolated in pure, 29.1 % were Gramnegative bacilli (GNB) isolated in pure and 15.2 % were a mixture of Gram-positive cocci and Gram-negative bacilli. Staphylococcus aureus 45 (80.4 %) was the predominant isolate followed by Coagulase Negative staphylococci 11(19.6 %). Among the GNB (35), the predominant isolate was Pseudomonas aeruginosa 10 (28.6 %) followed by Klebsiella pneumoniae 8 (22.9 %). Infections occurred during the early post-operative period in 63 % cases. Methicillin resistant Staphylococcus aureus were 53.4 % and 35 % were extended spectrum beta lactamases (ESBL) among Enterobacteriaceae strains. CONCLUSIONS Orthopaedic implant site infections are common during early post-operative period. Methicillin resistant staphylococcus and ESBL strains were high. This study of aerobic bacterial analysis and their current antibiogram of orthopaedic implant infections would greatly help the orthopaedic surgeons in selecting appropriate antibiotics for prophylaxis as well as better management of patients. KEYWORDS Orthopaedic Implants, MRSA, ESBL, Orthopaedic Implant Infections, Biofilm

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Characterisation of Aerobic Bacteriological Isolates From Orthopedic Implant Site Infections With Special Reference to Biofilm Formation in a Teritiary Care Hospital

Cited by 3 publications

References 10 publications

Detection of Biofilm Production and Antibiotic Resistance Pattern in Clinical Isolates from Orthopaedic Implants Associated Infections

Detection of Biofilm Production and Antibiotic Resistance Pattern in Clinical Isolates from Orthopaedic Implants Associated Infections

Prevalence of bacterial isolates causing surgical site infection with an emphasis on their biofilm forming capacity

Bacterial Aetiological Agents in Infected Orthopaedic Implants - A Cross Sectional Study from Andhra Medical College, Visakhapatnam

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