1992
DOI: 10.1071/pp9920043
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Characterisation of a Variegated Grapevine Mutant Showing Reduced Polyphenol Oxidase Activity

Abstract: Fruit of the variegated grapevine mutant Bruce's Sport is known to dry to a lighter colour than other seedless varieties. The biochemical basis for this decreased browning capacity was investigated. Bruce's Sport grapes had similar levels of phenolic compounds to Sultana H5. Activity of the enzyme polyphenol oxidase (PPO EC 1.10.3.1) in mature berries of Bruce's Sport was only 20-30% of that in Sultana H5. No evidence of inhibitors or activators of PPO was found when berry extracts were mixed. PPO activity on … Show more

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Cited by 17 publications
(22 citation statements)
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“…1). This was consistent with our earlier polarographic measurements of PPO activity in Sultana and Bruce's Sport berries (16).…”
Section: Resultssupporting
confidence: 81%
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“…1). This was consistent with our earlier polarographic measurements of PPO activity in Sultana and Bruce's Sport berries (16).…”
Section: Resultssupporting
confidence: 81%
“…This pattern of PPO gene expression correlates well with previous measurements of PPO activity in developing berries of Sultana and Bruce's Sport (16). As Sultana berries developed, PPO activity per berry, which provides an indication of PPO synthesis, increased markedly until the berries reached approximately 11 mm in diameter.…”
Section: Northern Blot Analysissupporting
confidence: 73%
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“…Les surnageants obtenus après centrifugation sont récupérés et le dosage a été effectué par la méthode au réactif de Folin Ciocalteu à 750 nm, en présence d'une gamme étalon d'acide caféique (Rathjen et al, 1992). renferme les échantillons E4, E7 et E8.…”
Section: Materiels Et Methodesunclassified