Characterisation of a secondary alcohol dehydrogenase from Xanthomonas campestris DSM 3586

Abstract

The chromosomal locus NP_636946 of Xanthomonas campestris DSM 3586 (ATCC 33913) which was earlier presumed to encode a quinoprotein glucose dehydrogenase has been cloned, expressed in Escherichia coli and the recombinant enzyme has been characterised. It was found to have no glucose dehydrogenase activity but to be active on many different polyols and diols, aliphatic alcohols, certain aldonic acids and amino-sugars. The product of D: -gluconic acid oxidation was 5-keto-D: -gluconic acid. The enzyme differs from polyol/gluconate dehydrogenases found in Gluconobacter by its single-chain architecture, different substrate specificity and much higher (20- to 30-fold) expression level in E.coli.

Membrane-Bound, 2-Keto- d -Gluconate-Yielding d -Gluconate Dehydrogenase from “ Gluconobacter dioxyacetonicus ” IFO 3271: Molecular Properties and Gene Disruption

“…(11). On the other hand, 5KGA production has been found exclusively in Gluconobacter so far, except that the secondary alcohol dehydrogenase of Xanthomonas campestris is reported to oxidize GA to produce 5KGA (17).…”

Selection of microbial biocatalysts for the reduction of cyclic and heterocyclic ketones

“…(CECT 3145), and Xanthomonas fragariae (CECT 549). There are some previous reports on the use of Erwinia [36], Streptomyces [37], or Xanthomonas [38] for the reduction of different aliphatic ketones or and β-ketoesters, but to the best of our knowledge, there are no reports on the reduction of cyclic ketones using these biocatalysts. Moreover, the conversion rates obtained in the present work are relatively high for non-modified wild-type microorganisms.…”

Preparative Biotransformations: Oxidation of Alcohols

“…Secondary alcohol dehydrogenase from Xanthomonas campestris DSM 3586 has been recombinantly produced and functionally characterized: higher conversion in shorter times of D ‐Gluconic acid to 5‐keto‐ D ‐Gluconic acid, compared to a similar enzyme from Gluconobacter oxydans, was obtained. It has no activity on glucose, but it could also be a useful biotechnological tool for the production of L ‐sorbose, rare pentuloses (such as L ‐ribulose), and amino‐sugar derivatives 67…”