Characterisation of a microbial community associated with a deep, coal seam methane reservoir in the Gippsland Basin, Australia

Midgley, David J.; Hendry, Philip; Pinetown, Kaydy; Fuentes, David; Gong, Shengkai; Mitchell, Danielle L.; Faiz, Mohinudeen

doi:10.1016/j.coal.2010.01.009

Cited by 108 publications

(55 citation statements)

References 21 publications

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“…Most of the bacterial pyrosequencing reads (75.07-97.1%) belonged to the phylum Proteobacteria in all samples as previously observed in other CBM reservoir basins (Fig. 3) (Midgley et al, 2010;Penner et al, 2010;Shimizu et al, 2007). The dominant Proteobacteria in the water sample were the genera Arcobacter (60.40%) and Azonexus (32.61%), which were also found in the rock sample with respectively 0.01% and 0.02% of the sequence reads (Table 2).…”

Section: Microflora Observed With Pyrosequencingsupporting

confidence: 58%

“…Since Shimizu et al (2007) initially reported the microbial methanogenesis and community structure in coal seam groundwater in Japan, microbial communities related to CBM have been studied worldwide (Midgley et al, 2010;Penner et al, 2010;Strapoć et al, 2008). However, most such studies used conventional molecular methods and sampled produced water drilled from CBM wells.…”

Section: Discussionmentioning

confidence: 99%

“…Several phylogenetic studies have been made regarding the microbial communities associated with CBM in such reservoirs as the Gippsland Basin (Midgley et al, 2010), the Illinois Basin (Strapoć et al, 2008), the northern Japan (Shimizu et al, 2007), and the western Canada (Penner et al, 2010). The most common archaeal genera detected in CBM reservoirs are Methanosarcina, Methanolobus, Methanobacteria, Methanocorpusculum, Methanosaeta, Methanococci, Methanoculleus, and Methanoregula.…”

Section: Introductionmentioning

confidence: 99%

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Pyrosequencing reveals the dominance of methylotrophic methanogenesis in a coal bed methane reservoir associated with Eastern Ordos Basin in China

Guo

Liu

et al. 2012

International Journal of Coal Geology

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a b s t r a c t a r t i c l e i n f oIt is generally believed that biogenic coal bed methane (CBM) is an end product of coal biodegradation by methanogenic archaea and syntrophic bacteria. In this work, the archaeal and bacterial communities of CBM reservoir associated with Ordos Basin in China were investigated using 454 pyrosequencing. Sampling produced water, coal and rock in the reservoir, a total of 46,598 sequence reads were obtained. All archaea were methanogens with the genus Methanolobus predominating. The genus consisted of 81.18% of pyrosequencing reads in water sample and > 99% in coal and rock samples. Although the phylum Proteobacteria was the main component of all samples, bacterial communities in coal and rock samples were similar at the genus level, which were distinctly separated with water sample. The results strongly suggested that methylotrophic methanogenesis governed the biogenic CBM formation. The separation of microbial communities between water and coal, rock samples should be considered when investigating the process of coal biodegradation and the generation of new biogenic CBM.

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Section: Microflora Observed With Pyrosequencingsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Pyrosequencing reveals the dominance of methylotrophic methanogenesis in a coal bed methane reservoir associated with Eastern Ordos Basin in China

Guo

Liu

et al. 2012

International Journal of Coal Geology

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“…Sampling gas and water produced in the Illinois Basin, Strapoć et al (2007Strapoć et al ( , 2008 demonstrated the predominance of biogenic CBM formed via hydrogentrophic methanogenesis by a population of Methanocorpusculum using geochemical and microbiological methods. It was found that methanogens were also present in water produced from other reservoirs, e.g., the Powder River Basin (Green et al 2008), the Gippsland Basin (Midgley et al 2010), and the Alberta Basin (Penner et al 2010). Furthermore, enrichment studies demonstrated that native methanogens indicated the production of methane by supplementing water samples with nutrients and substrates for methanogens (Green et al 2008;Midgley et al 2010).…”

Section: Introductionmentioning

confidence: 96%

Methylotrophic methanogenesis governs the biogenic coal bed methane formation in Eastern Ordos Basin, China

Guo

Liu

et al. 2012

Appl Microbiol Biotechnol

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To identify the methanogenic pathways present in a deep coal bed methane (CBM) reservoir associated with Eastern Ordos Basin in China, a series of geochemical and microbiological studies was performed using gas and water samples produced from the Liulin CBM reservoir. The composition and stable isotopic ratios of CBM implied a mixed biogenic and thermogenic origin of the methane. Archaeal 16S rRNA gene analysis revealed the dominance of the methylotrophic methanogen Methanolobus in the water produced. The high potential of methane production by methylotrophic methanogens was found in the enrichments using the water samples amended with methanol and incubated at 25 and 35°C. Methylotrophic methanogens were the dominant archaea in both enrichments as shown by polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE). Bacterial 16S rRNA gene analysis revealed that fermentative, sulfate-reducing, and nitrate-reducing bacteria inhabiting the water produced were a factor in coal biodegradation to fuel methanogens. These results suggested that past and ongoing biodegradation of coal by methylotrophic methanogens and syntrophic bacteria, as well as thermogenic CBM production, contributed to the Liulin CBM reserves associated with the Eastern Ordos Basin.

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“…Moreover, laboratory studies have demonstrated specificity between indigenous microbial consortia and coal type in some settings (e.g. Barik et al, 1991;Machnikowska et al, 2002;Midgley et al, 2010). The selective degradation of hydrogen-rich liptinite was demonstrated to effect methanogenesis from Texas lignite (Barik et al, 1991).…”

Section: Bioavailabilitymentioning

confidence: 99%

Conceptual exploration targeting for microbially enhanced coal bed methane (MECoM) in the Walloon Subgroup, eastern Surat Basin, Australia

Hamilton

Golding

Baublys

et al. 2015

International Journal of Coal Geology

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Characterisation of a microbial community associated with a deep, coal seam methane reservoir in the Gippsland Basin, Australia

Cited by 108 publications

References 21 publications

Pyrosequencing reveals the dominance of methylotrophic methanogenesis in a coal bed methane reservoir associated with Eastern Ordos Basin in China

Pyrosequencing reveals the dominance of methylotrophic methanogenesis in a coal bed methane reservoir associated with Eastern Ordos Basin in China

Methylotrophic methanogenesis governs the biogenic coal bed methane formation in Eastern Ordos Basin, China

Conceptual exploration targeting for microbially enhanced coal bed methane (MECoM) in the Walloon Subgroup, eastern Surat Basin, Australia

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