Characterisation of a Hydroxycinnamic Acid Esterase From the Bifidobacterium longum subsp. longum Taxon

Kelly, Sandra M.; O’Callaghan, J.R.; Kinsella, Michael; Sinderen, Douwe van

doi:10.3389/fmicb.2018.02690

Cited by 22 publications

(20 citation statements)

References 88 publications

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“…Bifidobacteria, like other members of the gut microbiota, possess ‘Carbohydrate Active Enzymes’ (CAZymes), such as GHs that break the glycosidic bonds between carbohydrate moieties and covalent linkages between carbohydrates and non-carbohydrate moieties. Carbohydrate esterases (CE) cleave the ester bound between a HCA and a carbohydrate residue, and thereby may provide access to other GHs to hydrolyse plant-derived oligosaccharides ( Kelly et al, 2018 ).…”

Section: Enzymatic Degradation Of Plant-oligosaccharides By Bifidobacmentioning

confidence: 99%

“…longum subsp. longum , whose encoding gene is located within the same genetic locus as the genes encoding GH enzymes that are predicted to be involved in plant-oligosaccharide utilization ( Raimondi et al, 2015 ; Fritsch et al, 2017 ; Kelly et al, 2018 ).…”

Section: Ax Axos Arabinan Arabinogalactan and Corn Gaxmentioning

confidence: 99%

“…Previously, esterases that cleave synthetic HCA substrates have been reported in bifidobacteria ( Raimondi et al, 2015 ; Fritsch et al, 2017 ; Kelly et al, 2018 ). The gene encoding the CaeA esterase is located in a locus predicted to be involved in AOS metabolism ( Arzamasov et al, 2018 ; Kelly et al, 2018 ). Removal of HCAs from plant-derived oligosaccharides is hypothesized to provide substrate access to GHs that might otherwise be sterically hindered by HCA substitutions.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

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Plant Glycan Metabolism by Bifidobacteria

2021

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Members of the genus Bifidobacterium, of which the majority have been isolated as gut commensals, are Gram-positive, non-motile, saccharolytic, non-sporulating, anaerobic bacteria. Many bifidobacterial strains are considered probiotic and therefore are thought to bestow health benefits upon their host. Bifidobacteria are highly abundant among the gut microbiota of healthy, full term, breast-fed infants, yet the relative average abundance of bifidobacteria tends to decrease as the human host ages. Because of the inverse correlation between bifidobacterial abundance/prevalence and health, there has been an increasing interest in maintaining, increasing or restoring bifidobacterial populations in the infant, adult and elderly gut. In order to colonize and persist in the gastrointestinal environment, bifidobacteria must be able to metabolise complex dietary and/or host-derived carbohydrates, and be resistant to various environmental challenges of the gut. This is not only important for the autochthonous bifidobacterial species colonising the gut, but also for allochthonous bifidobacteria provided as probiotic supplements in functional foods. For example, Bifidobacterium longum subsp. longum is a taxon associated with the metabolism of plant-derived poly/oligosaccharides in the adult diet, being capable of metabolising hemicellulose and various pectin-associated glycans. Many of these plant glycans are believed to stimulate the metabolism and growth of specific bifidobacterial species and are for this reason classified as prebiotics. In this review, bifidobacterial carbohydrate metabolism, with a focus on plant poly-/oligosaccharide degradation and uptake, as well as its associated regulation, will be discussed.

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Section: Enzymatic Degradation Of Plant-oligosaccharides By Bifidobacmentioning

confidence: 99%

Section: Ax Axos Arabinan Arabinogalactan and Corn Gaxmentioning

confidence: 99%

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

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Plant Glycan Metabolism by Bifidobacteria

2021

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“…Furthermore, the gut microbiota can metabolize certain complex, plant-derived carbohydrates that are common in the (adult) human diet. While few studies have focused on FAEs, Kelly et al (2018) have discovered a new hydroxycinnamic acid esterase that may be beneficial for the gut environment via Bifidobacterium longum subsp. longum.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Xylooligosaccharide, Xylan, and Whole Wheat Bran on the Human Gut Bacteria

et al. 2020

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Wheat bran is a cereal rich in dietary fibers that have high levels of ferulic acid, which has prebiotic effects on the intestinal microbiota and the host. Herein we explored the effect of xylooligosaccharide, xylan, and whole wheat bran on the human gut bacteria and screened for potential ferulic acid esterase genes. Using in vitro fermentation, we analyzed the air pressure, pH-value, and short-chain fatty acid levels. We also performed 16S rRNA gene and metagenomic sequencing. A Venn diagram analysis revealed that 80% of the core operational taxonomic units (OTUs) were shared among the samples, and most of the xylooligosaccharide treatment core OTUs (319/333 OTUs) were shared with the other two treatments’ core OTUs. A significant difference analysis revealed that the relative abundance of Dorea, Bilophila, and Sulfurovum in wheat bran treatment was higher than that in xylan and xylooligosaccharide treatments. The clusters of orthologous groups of proteins functional composition of all samples was similar to the microbiota composition of the control. Using metagenomic sequencing, we revealed seven genes containing the conserved residues, Gly-X-Ser-X-Gly, and the catalytic triad, Ser-His-Asp, which are thus potential ferulic acid esterase genes. All the results indicate that xylan and/or xylooligosaccharide, the main dietary fibers in wheat bran, plays a major role in in vitro fermentation by the human gut microbiota.

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“…Moreover, the esterase CaeA from B. longum subsp. longum was able to cleave several HCAs, although the hydrolytic action of B. longum against CGA has yet to be proven in in vitro colonic fermentation trials (Kelly, O'Callaghan, Kinsella, & van Sinderen, 2018).…”

Section: Metabolism and Biotransformation Of Hca By Gut Microbesmentioning

confidence: 99%

Hydroxycinnamic acids on gut microbiota and health

Leonard

Zhang

Ying

et al. 2020

Comp Rev Food Sci Food Safe

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Hydroxycinnamic acids (HCAs) are a major class of phenolic acids with the characteristic phenylpropanoid C6‐C3 backbone. Its typically conjugated status with plant cell wall components and liberation by limited enzymes might be the reason for its neglect by researchers compared to flavonoid‐type polyphenols. The polyphenol–gut microbiota interactions and their impact on human health have captured the interest of researchers recently. In addition, there has been a significant progress over the past few years in understanding the gut microbiota‐modulating effect of HCA using animal model studies. This review discusses the metabolism of HCA in the digestive tract, HCA–gut microbiota interactions, and its link to colorectal cancer, inflammatory bowel diseases, mental‐cognitive impairments, nonalcoholic liver disease, and obesity. The effects of food matrix and processing technologies on HCA bioavailability and HCA–gut microbiota interactions, and HCA safety concerns are also featured in this review. This paper has provided an in‐depth insight on HCA–gut microbiota relationship and identified the current literature gaps for future research.

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Characterisation of a Hydroxycinnamic Acid Esterase From the Bifidobacterium longum subsp. longum Taxon

Cited by 22 publications

References 88 publications

Plant Glycan Metabolism by Bifidobacteria

Plant Glycan Metabolism by Bifidobacteria

The Effect of Xylooligosaccharide, Xylan, and Whole Wheat Bran on the Human Gut Bacteria

Hydroxycinnamic acids on gut microbiota and health

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