Characterisation of a functional allatotropin receptor in the bumblebee, Bombus terrestris (Hymenoptera, Apidae)

Verlinden, Heleen; Lismont, Els; Bil, Magdalena; Urlacher, Elodie; Mercer, Alison R.; Broeck, Jozef Vanden; Huybrechts, Roger

doi:10.1016/j.ygcen.2013.08.006

Cited by 22 publications

(14 citation statements)

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“…The obtained sequence has considerable similarity with orthologous receptors from other insects (Figure 1) (Horodyski et al, 2011; Vuerinckx et al, 2011; Nouzova et al, 2012; Verlinden et al, 2013). The sequence contains a DRW (instead of the typical DRY) motif in the second intracellular loop, as it is the case for other AT receptors.…”

Section: Discussionmentioning

confidence: 68%

“…It was first thought that the hymenopteran insects, A. mellifera (Hummon et al, 2006) and N. vitripennis (Hauser et al, 2010) also lacked an AT-like peptide. However, after a more thorough search, a hymenopteran AT gene was found (Veenstra et al, 2012) and, meanwhile, an AT receptor was characterized in the hymenopteran species B. terrestris (Verlinden et al, 2013). …”

Section: Discussionmentioning

confidence: 99%

“…The AT receptors (ATRs) are orthologous to vertebrate orexin/hypocretin receptors. To date, five ATRs have been characterized; namely the neuropeptide 16 receptor in Bombyx mori (Yamanaka et al, 2008), the receptors of M. sexta (Horodyski et al, 2011), Tribolium castaneum (Vuerinckx et al, 2011), Aedes aegypti (Nouzova et al, 2012) and Bombus terrestris (Verlinden et al, 2013). The first three were dose-dependently activated by Manse -AT.…”

Section: Introductionmentioning

confidence: 99%

“…The T. castaneum receptor was also activated by Schgr -AT (which is identical to Lom -AG-MT1, the AT from Locusta migratoria ) and by an endogenous AT-like peptide ( Trica -ATL) predicted from the Tribolium genome (Vuerinckx et al, 2011). The ATR receptor of B. terrestris also responded to Manse-AT , Schgr -AT, and Trica -ATL, but much higher concentrations were needed for generating these pharmacological effects (Verlinden et al, 2013). Activation of these AT(L) receptors resulted in an elevation of intracellular calcium and cAMP concentrations (Horodyski et al, 2011; Vuerinckx et al, 2011; Verlinden et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…The ATR receptor of B. terrestris also responded to Manse-AT , Schgr -AT, and Trica -ATL, but much higher concentrations were needed for generating these pharmacological effects (Verlinden et al, 2013). Activation of these AT(L) receptors resulted in an elevation of intracellular calcium and cAMP concentrations (Horodyski et al, 2011; Vuerinckx et al, 2011; Verlinden et al, 2013). …”

Section: Introductionmentioning

confidence: 99%

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Molecular cloning and characterization of the allatotropin precursor and receptor in the desert locust, Schistocerca gregaria

et al. 2015

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Allatotropins (ATs) are pleiotropic neuropeptides initially isolated from the tobacco hornworm, Manduca sexta. In 2008, the first receptor for AT-like peptides (ATR) was characterized in Bombyx mori. Since then, ATRs have also been characterized in M. sexta, Tribolium castaneum, Aedes aegypti and Bombus terrestris. These receptors show sequence similarity to vertebrate orexin (ORX) receptors. When generating an EST-database of the desert locust (Schistocerca gregaria) central nervous system, we found cDNA sequences encoding the Schgr-AT precursor and a fragment of its putative receptor. This receptor cDNA has now been completed and functionally expressed in mammalian cell lines. Activation of this receptor, designated as Schgr-ATR, by Schgr-AT caused an increase in intracellular calcium ions, as well as cyclic AMP (cAMP), with an EC50 value in the nanomolar range. In addition, the transcript distribution of both the Schgr-AT precursor and Schgr-ATR was investigated by means of quantitative real-time PCR. Moreover, we found more evidence for the myotropic and allatostimulatory actions of Schgr-AT in the desert locust. These data are discussed and situated in a broader context by comparison with literature data on AT and ATR in insects.

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