Characterisation of a cyclin-dependent kinase (CDKA) gene expressed during somatic embryogenesis of coconut palm

Abstract

The CDKA gene is linked to the cellular control. This gene was isolated from coconut palm (Cocos nucifera L) and a detailed expression analysis was done during somatic embryogenesis. Analysis of the deduced amino acid sequence showed the most important residues to be conserved. The highest homology was with Picea abies (96% similarity). Expression of the putative CnCDKA gene steadily increased during embryogenic callus formation phase when embryogenic competence is attained. In situ hybridization specified the localization of the transcripts as being mainly in a few cell layers within the meristematic centres in embryogenic calli at 90 day cultures. Analysis of CnCDKA expression at different somatic embryo formation stages showed that the expression was decreased progressively with the lowest expression in germinated somatic embryos. (Résumé d'auteur

Histological analysis and transcription profiles on somatic embryogenesis in interspecific hybrids of <i>Elaeis guineensis × E. oleifera</i>

“…Histological and reverse transcription techniques were used to complement morphology as support for decisions, going deeper on the comprehension of embryogenesis and multiplication/maturation of embryogenic cultures, and to institute molecular markers to assist selection of the best callus lines, maybe among thousands if numerous progenies are going to be cloned. An example would be the CYCLIN DEPENDENT KINASE from coconut palm, which increased expression, marked the emergence of "ear"-like embryogenic structures [30].…”

“…Rooting was tested on 76 shoots from poly-embryogenic complexes and on 38 shoots from mechanically individualized somatic embryos, in basal medium free of casein or glutamine, containing half the concentration of iron, supplemented with 30 ). Explants were cultivated on 50 ml medium/500 ml flask or 15 ml medium/ 22.5 × 2.5 cm culture tubes.…”

Biology, propagation and utilization of elite coconut varieties (makapuno and aromatics)

“…By following the expression of particular genes during the embryogenesis process, the early selection of the appropriate cell culture lines most likely to undergo SE can be achieved. A range of marker genes have been discovered, including CnSERK, CnANT, CnCDKA [84][85][86] that can indicate those cell culture lines that are most likely to undergo SE. In addition, DNA-based markers can also be used to assess the extent of somaclonal variation within the SE-derived plantlets using Random Amplification of Polymorphic DNA (RAPD), Simple Sequence Repeats (SSR) or Amplified Fragment Length Polymorphism (AFLP) analysis.…”

Comparative gene expression profiling during in vitro regeneration in two coconut cultivars

“…In recalcitrant species such as coconut, the differential gene expression studies during somatic embryogenesis are limited and focussed on a few genes like cyclin-dependent kinase gene (CDKA) (Montero-Cortes et al, 2010a), KNOTTEDlike homeobox gene (Montero-Corte et al, 2010b) and SERK gene (Perez-Nunez et al, 2009). In coconut, transcriptome analysis of embryogenic calli derived from plumular explants of West Coast Tall (WCT) cultivar resulted in the identifi cation of fourteen genes; the expression patterns of these genes were validated in six development stages using RT-qPCR .…”