Characterisation and expression of SPLUNC2, the human orthologue of rodent parotid secretory protein

Bingle, Colin D.; Barnes, Frances A.; Lunn, Hayley; Musa, Maslinda; Webster, Steve J.; Douglas, C. W. I.; Cross, Simon S.; High, A.S.; Bingle, Colin D.

doi:10.1007/s00418-009-0610-4

Cited by 37 publications

(51 citation statements)

References 33 publications

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“…58 BPIFA2 is a heavily N-glycosylated protein, 14 and on a western blot, the two upper bands correspond to the glycosylated form of BPIFA2, whereas the lower band represents the nonglycosylated form. In the present study, we observed a change in the glycosylation pattern of BPIFA2, as the patients with cancer expressed higher levels of the glycosylated form of BPIFA2 and lower levels of the nonglycosylated form compared with controls.…”

Section: ; 34 ; 52mentioning

confidence: 99%

Salivary BPIFA1 (SPLUNC1) and BPIFA2 (SPLUNC2 A) are modified by head and neck cancer radiotherapy

González‐Arriagada

Ramos

Silva

et al. 2015

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

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Objective: To determine the effects of radiotherapy on salivary BPIFA expression and to investigate the role of BPIFA in the development of known radiotherapy side effects. Materials and Methods:Unstimulated whole-mouth saliva was collected from 45 cancer patients (1 week before treatment, during the treatment, and 1 week after completion of radiotherapy) and from 20 controls. BPIFA1 and BPIFA2 expression was detected by western blotting and analyzed along with clinicopathologic data and side effects from the radiotherapy.Results: A facial radiation field was associated with lower salivary flow during and after radiotherapy and correlated with side effects, mainly mucositis. Salivary BPIFA1 expression levels were similar between the control group and the patient group before treatment. On the other hand, BPIFA2 levels were higher in the patient group before treatment compared with the control group. BPIFA concentration was modified by radiotherapy as BPIFA1 levels increased (P = .0081) and BPIFA2 decreased (P < .0001). Higher levels of BPIFA1 were associated with the presence of mucositis (P = .0363) and its severity (P = .0500). Conclusions:The present study found that levels of BPIFA1 and glycosylated forms of BPIFA2 are affected by radiotherapy, suggesting that these proteins may play a role in the oral microenvironment in irradiated patients with head and neck cancer. Statement of Clinical Relevance:The present study which showed that BPIFA proteins expression was modified in patients who underwent radiotherapy may contribute to better understanding the etiology and could be useful to improve the management of its side effects of radiotherapy.

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Section: ; 34 ; 52mentioning

confidence: 99%

Salivary BPIFA1 (SPLUNC1) and BPIFA2 (SPLUNC2 A) are modified by head and neck cancer radiotherapy

González‐Arriagada

Ramos

Silva

et al. 2015

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

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“…Secreted PSP has antibacterial activity which contributes to protection of the oral cavity (Gorr et al 2011). Human PSP (SPLUNC2, BPIFA2) has been shown to be expressed in saliva as several isoforms due to alternative splicing of the mRNA (Bingle et al 2009;Bingle et al 2011). Another abundant salivary protein, amylase, initiates digestion of starch, and also adheres to oral bacteria and enamel.…”

Section: Sorting For Regulated Secretion In the Parotid Glandmentioning

confidence: 99%

Phosphatidylinositol Bisphosphate Mediated Sorting of Secretory Granule Cargo

Darling¹,

Venkatesh²,

Goyal³

et al. 2012

Crosstalk and Integration of Membrane Trafficking Pathways

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“…Like rat PSP, SPLUNC2 is expressed predominantly in parotid and submandibular glands. It has also been shown to be produced by minor salivary glands [69]. Its presence in whole saliva has been reported by many proteomic studies [70].…”

Section: Parotid Secretory Proteinmentioning

confidence: 85%

“…PSP is a member of the Palate Lung-Nasal epithelium clone (PLUNC) gene sub- SPLUNC 2 is the human orthologue of rodent PSP with 22% similar and 52% identical protein sequence [69]. Like rat PSP, SPLUNC2 is expressed predominantly in parotid and submandibular glands.…”

Section: Parotid Secretory Proteinmentioning

confidence: 99%

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Direct binding of parotid secretory protein to phosphatidylinositol phosphates.

Goyal¹

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Characterisation and expression of SPLUNC2, the human orthologue of rodent parotid secretory protein

Cited by 37 publications

References 33 publications

Salivary BPIFA1 (SPLUNC1) and BPIFA2 (SPLUNC2 A) are modified by head and neck cancer radiotherapy

Salivary BPIFA1 (SPLUNC1) and BPIFA2 (SPLUNC2 A) are modified by head and neck cancer radiotherapy

Phosphatidylinositol Bisphosphate Mediated Sorting of Secretory Granule Cargo

Direct binding of parotid secretory protein to phosphatidylinositol phosphates.

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